Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0759 and EX28DRAFT_0760 are separated by 47 nucleotides EX28DRAFT_0760 and EX28DRAFT_0761 are separated by 93 nucleotides EX28DRAFT_0761 and EX28DRAFT_0762 are separated by 123 nucleotides EX28DRAFT_0762 and EX28DRAFT_0763 are separated by 143 nucleotides
EX28DRAFT_0759: EX28DRAFT_0759 - Uncharacterized protein conserved in bacteria, at 755,896 to 756,171
_0759
EX28DRAFT_0760: EX28DRAFT_0760 - 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway), at 756,219 to 756,878
_0760
EX28DRAFT_0761: EX28DRAFT_0761 - Uncharacterized conserved protein, at 756,972 to 757,412
_0761
EX28DRAFT_0762: EX28DRAFT_0762 - Disulfide bond formation protein DsbB, at 757,536 to 758,066
_0762
EX28DRAFT_0763: EX28DRAFT_0763 - Na+/H+ antiporter NhaB, at 758,210 to 759,748
_0763
Position (kb) 756
757
758 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 755.988 kb on - strand, within EX28DRAFT_0759 at 756.042 kb on + strand, within EX28DRAFT_0759 at 756.043 kb on - strand, within EX28DRAFT_0759 at 756.100 kb on + strand, within EX28DRAFT_0759 at 756.100 kb on + strand, within EX28DRAFT_0759 at 756.103 kb on + strand, within EX28DRAFT_0759 at 756.107 kb on + strand, within EX28DRAFT_0759 at 756.189 kb on + strand at 756.223 kb on - strand at 756.233 kb on + strand at 756.234 kb on - strand at 756.234 kb on - strand at 756.295 kb on - strand, within EX28DRAFT_0760 at 756.354 kb on - strand, within EX28DRAFT_0760 at 756.354 kb on - strand, within EX28DRAFT_0760 at 756.354 kb on - strand, within EX28DRAFT_0760 at 756.720 kb on - strand, within EX28DRAFT_0760 at 756.741 kb on + strand, within EX28DRAFT_0760 at 756.764 kb on + strand, within EX28DRAFT_0760 at 756.765 kb on - strand, within EX28DRAFT_0760 at 756.833 kb on - strand at 757.129 kb on + strand, within EX28DRAFT_0761 at 757.130 kb on - strand, within EX28DRAFT_0761 at 757.158 kb on + strand, within EX28DRAFT_0761 at 757.158 kb on + strand, within EX28DRAFT_0761 at 757.238 kb on + strand, within EX28DRAFT_0761 at 757.238 kb on + strand, within EX28DRAFT_0761 at 757.248 kb on + strand, within EX28DRAFT_0761 at 757.331 kb on + strand, within EX28DRAFT_0761 at 757.331 kb on + strand, within EX28DRAFT_0761 at 757.537 kb on + strand at 757.935 kb on - strand, within EX28DRAFT_0762 at 758.067 kb on - strand at 758.089 kb on + strand at 758.089 kb on + strand at 758.090 kb on - strand at 758.144 kb on - strand at 758.150 kb on + strand at 758.207 kb on - strand at 758.207 kb on - strand at 758.207 kb on - strand at 758.207 kb on - strand at 758.207 kb on - strand at 758.207 kb on - strand at 758.207 kb on - strand at 758.207 kb on - strand at 758.207 kb on - strand at 758.225 kb on + strand at 758.225 kb on + strand at 758.225 kb on + strand at 758.227 kb on + strand at 758.228 kb on - strand at 758.238 kb on - strand at 758.266 kb on + strand at 758.308 kb on + strand at 758.308 kb on + strand at 758.310 kb on + strand at 758.351 kb on + strand at 758.352 kb on - strand at 758.352 kb on - strand at 758.352 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 755,988 - EX28DRAFT_0759 0.33 +0.8 756,042 + EX28DRAFT_0759 0.53 -3.3 756,043 - EX28DRAFT_0759 0.53 +1.1 756,100 + EX28DRAFT_0759 0.74 -2.5 756,100 + EX28DRAFT_0759 0.74 +0.3 756,103 + EX28DRAFT_0759 0.75 -1.9 756,107 + EX28DRAFT_0759 0.76 +1.4 756,189 + +0.3 756,223 - -0.4 756,233 + +1.1 756,234 - +0.3 756,234 - -3.2 756,295 - EX28DRAFT_0760 0.12 -0.3 756,354 - EX28DRAFT_0760 0.20 +0.9 756,354 - EX28DRAFT_0760 0.20 +0.5 756,354 - EX28DRAFT_0760 0.20 +2.1 756,720 - EX28DRAFT_0760 0.76 -1.7 756,741 + EX28DRAFT_0760 0.79 +0.1 756,764 + EX28DRAFT_0760 0.83 +1.3 756,765 - EX28DRAFT_0760 0.83 +1.2 756,833 - -0.5 757,129 + EX28DRAFT_0761 0.36 +0.5 757,130 - EX28DRAFT_0761 0.36 +1.7 757,158 + EX28DRAFT_0761 0.42 -0.2 757,158 + EX28DRAFT_0761 0.42 +0.1 757,238 + EX28DRAFT_0761 0.60 -2.3 757,238 + EX28DRAFT_0761 0.60 +0.3 757,248 + EX28DRAFT_0761 0.63 -0.9 757,331 + EX28DRAFT_0761 0.81 +3.9 757,331 + EX28DRAFT_0761 0.81 +0.1 757,537 + +0.8 757,935 - EX28DRAFT_0762 0.75 -0.9 758,067 - -0.7 758,089 + +1.4 758,089 + -1.5 758,090 - -4.2 758,144 - -0.0 758,150 + -2.3 758,207 - -0.9 758,207 - -3.3 758,207 - +0.7 758,207 - +1.1 758,207 - -0.7 758,207 - +0.1 758,207 - +0.1 758,207 - -2.2 758,207 - -1.3 758,225 + -0.2 758,225 + +0.7 758,225 + -0.6 758,227 + +0.7 758,228 - -2.3 758,238 - +0.1 758,266 + +0.1 758,308 + +2.2 758,308 + -0.8 758,310 + +0.4 758,351 + +0.3 758,352 - -1.9 758,352 - +1.1 758,352 - -2.3
Or see this region's nucleotide sequence