Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0485 and EX28DRAFT_0486 are separated by 130 nucleotides EX28DRAFT_0486 and EX28DRAFT_0487 overlap by 36 nucleotides
EX28DRAFT_0485: EX28DRAFT_0485 - Molecular chaperone, at 472,955 to 474,307
_0485
EX28DRAFT_0486: EX28DRAFT_0486 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 474,438 to 475,310
_0486
EX28DRAFT_0487: EX28DRAFT_0487 - PAS domain S-box/diguanylate cyclase (GGDEF) domain, at 475,275 to 478,604
_0487
Position (kb)
474
475
476 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 473.608 kb on + strand, within EX28DRAFT_0485 at 473.666 kb on + strand, within EX28DRAFT_0485 at 473.667 kb on - strand, within EX28DRAFT_0485 at 473.667 kb on - strand, within EX28DRAFT_0485 at 473.667 kb on - strand, within EX28DRAFT_0485 at 473.899 kb on + strand, within EX28DRAFT_0485 at 473.997 kb on + strand, within EX28DRAFT_0485 at 473.998 kb on - strand, within EX28DRAFT_0485 at 474.022 kb on - strand, within EX28DRAFT_0485 at 474.064 kb on + strand, within EX28DRAFT_0485 at 474.065 kb on - strand, within EX28DRAFT_0485 at 474.283 kb on + strand at 474.288 kb on + strand at 474.289 kb on - strand at 474.311 kb on - strand at 474.311 kb on - strand at 474.391 kb on + strand at 474.391 kb on + strand at 474.392 kb on - strand at 474.392 kb on - strand at 474.442 kb on - strand at 474.465 kb on + strand at 474.538 kb on - strand, within EX28DRAFT_0486 at 474.541 kb on - strand, within EX28DRAFT_0486 at 474.578 kb on - strand, within EX28DRAFT_0486 at 474.609 kb on - strand, within EX28DRAFT_0486 at 474.609 kb on - strand, within EX28DRAFT_0486 at 475.063 kb on + strand, within EX28DRAFT_0486 at 475.063 kb on + strand, within EX28DRAFT_0486 at 475.063 kb on + strand, within EX28DRAFT_0486 at 475.101 kb on + strand, within EX28DRAFT_0486 at 475.153 kb on - strand, within EX28DRAFT_0486 at 475.153 kb on - strand, within EX28DRAFT_0486 at 475.247 kb on + strand at 475.248 kb on - strand at 475.248 kb on - strand at 475.248 kb on - strand at 475.248 kb on - strand at 475.254 kb on + strand at 475.255 kb on - strand at 475.256 kb on + strand at 475.291 kb on + strand at 475.292 kb on - strand at 475.308 kb on + strand at 475.483 kb on + strand at 475.483 kb on + strand at 475.519 kb on + strand at 475.519 kb on + strand at 475.664 kb on + strand, within EX28DRAFT_0487 at 475.700 kb on + strand, within EX28DRAFT_0487 at 475.735 kb on - strand, within EX28DRAFT_0487 at 475.743 kb on + strand, within EX28DRAFT_0487 at 475.744 kb on - strand, within EX28DRAFT_0487 at 475.944 kb on - strand, within EX28DRAFT_0487 at 476.006 kb on + strand, within EX28DRAFT_0487 at 476.116 kb on + strand, within EX28DRAFT_0487 at 476.124 kb on + strand, within EX28DRAFT_0487 at 476.124 kb on + strand, within EX28DRAFT_0487 at 476.125 kb on - strand, within EX28DRAFT_0487 at 476.126 kb on + strand, within EX28DRAFT_0487 at 476.225 kb on + strand, within EX28DRAFT_0487 at 476.225 kb on + strand, within EX28DRAFT_0487 at 476.234 kb on - strand, within EX28DRAFT_0487 at 476.270 kb on - strand, within EX28DRAFT_0487
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 473,608 + EX28DRAFT_0485 0.48 -1.1 473,666 + EX28DRAFT_0485 0.53 +0.1 473,667 - EX28DRAFT_0485 0.53 -0.7 473,667 - EX28DRAFT_0485 0.53 -0.2 473,667 - EX28DRAFT_0485 0.53 -0.0 473,899 + EX28DRAFT_0485 0.70 +0.9 473,997 + EX28DRAFT_0485 0.77 +0.6 473,998 - EX28DRAFT_0485 0.77 +0.8 474,022 - EX28DRAFT_0485 0.79 +0.1 474,064 + EX28DRAFT_0485 0.82 -1.5 474,065 - EX28DRAFT_0485 0.82 +0.0 474,283 + -2.0 474,288 + -0.0 474,289 - +0.3 474,311 - +0.4 474,311 - -0.7 474,391 + -1.4 474,391 + -0.7 474,392 - -2.1 474,392 - +0.6 474,442 - -1.2 474,465 + -0.6 474,538 - EX28DRAFT_0486 0.11 -1.5 474,541 - EX28DRAFT_0486 0.12 -0.6 474,578 - EX28DRAFT_0486 0.16 -4.5 474,609 - EX28DRAFT_0486 0.20 -2.3 474,609 - EX28DRAFT_0486 0.20 +0.2 475,063 + EX28DRAFT_0486 0.72 -2.0 475,063 + EX28DRAFT_0486 0.72 -0.0 475,063 + EX28DRAFT_0486 0.72 -1.3 475,101 + EX28DRAFT_0486 0.76 -2.3 475,153 - EX28DRAFT_0486 0.82 -0.7 475,153 - EX28DRAFT_0486 0.82 -2.8 475,247 + +2.4 475,248 - -1.0 475,248 - +0.7 475,248 - -1.4 475,248 - +0.3 475,254 + -0.1 475,255 - -0.5 475,256 + +2.1 475,291 + -0.2 475,292 - +0.2 475,308 + -0.2 475,483 + -1.3 475,483 + -0.3 475,519 + +0.6 475,519 + +0.6 475,664 + EX28DRAFT_0487 0.12 +0.6 475,700 + EX28DRAFT_0487 0.13 -0.8 475,735 - EX28DRAFT_0487 0.14 +0.3 475,743 + EX28DRAFT_0487 0.14 -0.9 475,744 - EX28DRAFT_0487 0.14 +0.7 475,944 - EX28DRAFT_0487 0.20 -2.1 476,006 + EX28DRAFT_0487 0.22 -1.4 476,116 + EX28DRAFT_0487 0.25 +0.7 476,124 + EX28DRAFT_0487 0.25 -1.9 476,124 + EX28DRAFT_0487 0.25 -2.6 476,125 - EX28DRAFT_0487 0.26 -0.2 476,126 + EX28DRAFT_0487 0.26 +1.1 476,225 + EX28DRAFT_0487 0.29 +0.8 476,225 + EX28DRAFT_0487 0.29 +0.1 476,234 - EX28DRAFT_0487 0.29 -2.1 476,270 - EX28DRAFT_0487 0.30 +1.7
Or see this region's nucleotide sequence