Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0406 and EX28DRAFT_0407 are separated by 295 nucleotides EX28DRAFT_0407 and EX28DRAFT_0408 are separated by 79 nucleotides
EX28DRAFT_0406: EX28DRAFT_0406 - Transcriptional regulators, at 383,059 to 384,081
_0406
EX28DRAFT_0407: EX28DRAFT_0407 - ABC-type sugar transport system, periplasmic component, at 384,377 to 385,375
_0407
EX28DRAFT_0408: EX28DRAFT_0408 - ABC-type sugar transport system, ATPase component, at 385,455 to 386,975
_0408
Position (kb) 384
385
386 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 383.406 kb on + strand, within EX28DRAFT_0406 at 383.461 kb on - strand, within EX28DRAFT_0406 at 383.619 kb on + strand, within EX28DRAFT_0406 at 383.620 kb on - strand, within EX28DRAFT_0406 at 383.772 kb on + strand, within EX28DRAFT_0406 at 383.772 kb on + strand, within EX28DRAFT_0406 at 383.789 kb on - strand, within EX28DRAFT_0406 at 383.858 kb on + strand, within EX28DRAFT_0406 at 383.859 kb on - strand, within EX28DRAFT_0406 at 384.129 kb on + strand at 384.150 kb on - strand at 384.184 kb on + strand at 384.185 kb on - strand at 384.207 kb on + strand at 384.282 kb on + strand at 384.373 kb on + strand at 384.382 kb on + strand at 384.382 kb on + strand at 384.383 kb on - strand at 384.473 kb on + strand at 384.475 kb on + strand at 384.475 kb on + strand at 384.475 kb on + strand at 384.476 kb on - strand at 384.476 kb on - strand at 384.476 kb on - strand at 384.479 kb on + strand, within EX28DRAFT_0407 at 384.479 kb on + strand, within EX28DRAFT_0407 at 384.749 kb on + strand, within EX28DRAFT_0407 at 384.750 kb on - strand, within EX28DRAFT_0407 at 384.750 kb on - strand, within EX28DRAFT_0407 at 384.762 kb on - strand, within EX28DRAFT_0407 at 384.764 kb on + strand, within EX28DRAFT_0407 at 384.764 kb on + strand, within EX28DRAFT_0407 at 384.765 kb on - strand, within EX28DRAFT_0407 at 384.796 kb on + strand, within EX28DRAFT_0407 at 384.797 kb on - strand, within EX28DRAFT_0407 at 384.926 kb on + strand, within EX28DRAFT_0407 at 384.926 kb on + strand, within EX28DRAFT_0407 at 384.926 kb on + strand, within EX28DRAFT_0407 at 384.926 kb on + strand, within EX28DRAFT_0407 at 384.926 kb on + strand, within EX28DRAFT_0407 at 384.927 kb on - strand, within EX28DRAFT_0407 at 384.927 kb on - strand, within EX28DRAFT_0407 at 384.978 kb on + strand, within EX28DRAFT_0407 at 384.979 kb on - strand, within EX28DRAFT_0407 at 384.979 kb on - strand, within EX28DRAFT_0407 at 385.101 kb on + strand, within EX28DRAFT_0407 at 385.150 kb on - strand, within EX28DRAFT_0407 at 385.328 kb on + strand at 385.329 kb on - strand at 385.329 kb on - strand at 385.373 kb on + strand at 385.373 kb on + strand at 385.373 kb on + strand at 385.373 kb on + strand at 385.422 kb on + strand at 385.448 kb on + strand at 385.449 kb on - strand at 385.450 kb on + strand at 385.451 kb on - strand at 385.455 kb on - strand at 385.550 kb on + strand at 385.550 kb on + strand at 385.574 kb on + strand at 385.607 kb on + strand, within EX28DRAFT_0408 at 385.745 kb on - strand, within EX28DRAFT_0408 at 385.776 kb on - strand, within EX28DRAFT_0408 at 385.950 kb on + strand, within EX28DRAFT_0408 at 385.950 kb on + strand, within EX28DRAFT_0408 at 385.950 kb on + strand, within EX28DRAFT_0408 at 385.950 kb on + strand, within EX28DRAFT_0408 at 386.027 kb on + strand, within EX28DRAFT_0408 at 386.038 kb on + strand, within EX28DRAFT_0408 at 386.064 kb on + strand, within EX28DRAFT_0408 at 386.064 kb on + strand, within EX28DRAFT_0408 at 386.064 kb on + strand, within EX28DRAFT_0408 at 386.064 kb on + strand, within EX28DRAFT_0408 at 386.066 kb on + strand, within EX28DRAFT_0408 at 386.066 kb on + strand, within EX28DRAFT_0408 at 386.289 kb on - strand, within EX28DRAFT_0408
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 383,406 + EX28DRAFT_0406 0.34 +0.4 383,461 - EX28DRAFT_0406 0.39 +0.0 383,619 + EX28DRAFT_0406 0.55 -0.1 383,620 - EX28DRAFT_0406 0.55 -1.9 383,772 + EX28DRAFT_0406 0.70 +0.3 383,772 + EX28DRAFT_0406 0.70 -3.3 383,789 - EX28DRAFT_0406 0.71 +0.9 383,858 + EX28DRAFT_0406 0.78 -2.5 383,859 - EX28DRAFT_0406 0.78 +0.3 384,129 + -0.6 384,150 - +0.0 384,184 + +0.6 384,185 - -1.3 384,207 + +0.6 384,282 + +0.3 384,373 + +0.1 384,382 + -0.5 384,382 + +0.1 384,383 - +0.1 384,473 + -0.1 384,475 + +0.2 384,475 + +0.9 384,475 + -0.6 384,476 - +0.2 384,476 - -0.9 384,476 - +1.3 384,479 + EX28DRAFT_0407 0.10 +0.6 384,479 + EX28DRAFT_0407 0.10 +0.8 384,749 + EX28DRAFT_0407 0.37 -0.2 384,750 - EX28DRAFT_0407 0.37 +3.6 384,750 - EX28DRAFT_0407 0.37 -0.6 384,762 - EX28DRAFT_0407 0.39 -0.6 384,764 + EX28DRAFT_0407 0.39 -2.5 384,764 + EX28DRAFT_0407 0.39 -1.3 384,765 - EX28DRAFT_0407 0.39 +0.9 384,796 + EX28DRAFT_0407 0.42 +0.9 384,797 - EX28DRAFT_0407 0.42 -0.0 384,926 + EX28DRAFT_0407 0.55 +0.3 384,926 + EX28DRAFT_0407 0.55 +0.1 384,926 + EX28DRAFT_0407 0.55 -2.3 384,926 + EX28DRAFT_0407 0.55 +0.2 384,926 + EX28DRAFT_0407 0.55 +0.5 384,927 - EX28DRAFT_0407 0.55 +0.5 384,927 - EX28DRAFT_0407 0.55 -1.3 384,978 + EX28DRAFT_0407 0.60 -0.0 384,979 - EX28DRAFT_0407 0.60 -0.7 384,979 - EX28DRAFT_0407 0.60 -0.1 385,101 + EX28DRAFT_0407 0.72 +0.7 385,150 - EX28DRAFT_0407 0.77 -0.5 385,328 + -3.0 385,329 - -0.7 385,329 - -0.4 385,373 + -0.5 385,373 + -3.4 385,373 + +0.6 385,373 + +0.0 385,422 + +0.3 385,448 + +0.0 385,449 - -4.6 385,450 + +0.4 385,451 - -0.4 385,455 - -0.0 385,550 + -0.2 385,550 + -0.6 385,574 + +0.4 385,607 + EX28DRAFT_0408 0.10 +0.3 385,745 - EX28DRAFT_0408 0.19 +0.3 385,776 - EX28DRAFT_0408 0.21 +0.4 385,950 + EX28DRAFT_0408 0.33 -1.3 385,950 + EX28DRAFT_0408 0.33 -0.2 385,950 + EX28DRAFT_0408 0.33 -0.5 385,950 + EX28DRAFT_0408 0.33 -0.1 386,027 + EX28DRAFT_0408 0.38 +1.0 386,038 + EX28DRAFT_0408 0.38 +1.3 386,064 + EX28DRAFT_0408 0.40 +0.3 386,064 + EX28DRAFT_0408 0.40 -0.8 386,064 + EX28DRAFT_0408 0.40 -0.3 386,064 + EX28DRAFT_0408 0.40 +0.2 386,066 + EX28DRAFT_0408 0.40 -0.1 386,066 + EX28DRAFT_0408 0.40 -1.3 386,289 - EX28DRAFT_0408 0.55 -2.3
Or see this region's nucleotide sequence