Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0403 and EX28DRAFT_0404 are separated by 102 nucleotides EX28DRAFT_0404 and EX28DRAFT_0405 are separated by 19 nucleotides EX28DRAFT_0405 and EX28DRAFT_0406 are separated by 155 nucleotides
EX28DRAFT_0403: EX28DRAFT_0403 - Protein of unknown function (DUF1228), at 379,807 to 380,955
_0403
EX28DRAFT_0404: EX28DRAFT_0404 - GTP cyclohydrolase I, at 381,058 to 381,726
_0404
EX28DRAFT_0405: EX28DRAFT_0405 - Predicted membrane protein, at 381,746 to 382,903
_0405
EX28DRAFT_0406: EX28DRAFT_0406 - Transcriptional regulators, at 383,059 to 384,081
_0406
Position (kb) 381
382
383 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 380.770 kb on + strand, within EX28DRAFT_0403 at 380.770 kb on + strand, within EX28DRAFT_0403 at 380.770 kb on + strand, within EX28DRAFT_0403 at 380.771 kb on - strand, within EX28DRAFT_0403 at 380.771 kb on - strand, within EX28DRAFT_0403 at 380.773 kb on - strand, within EX28DRAFT_0403 at 380.954 kb on - strand at 380.954 kb on - strand at 380.957 kb on + strand at 380.958 kb on - strand at 381.808 kb on + strand at 381.809 kb on - strand at 381.811 kb on + strand at 381.812 kb on - strand at 381.922 kb on + strand, within EX28DRAFT_0405 at 382.063 kb on + strand, within EX28DRAFT_0405 at 382.128 kb on + strand, within EX28DRAFT_0405 at 382.128 kb on + strand, within EX28DRAFT_0405 at 382.141 kb on + strand, within EX28DRAFT_0405 at 382.160 kb on + strand, within EX28DRAFT_0405 at 382.161 kb on - strand, within EX28DRAFT_0405 at 382.161 kb on - strand, within EX28DRAFT_0405 at 382.161 kb on - strand, within EX28DRAFT_0405 at 382.521 kb on - strand, within EX28DRAFT_0405 at 382.601 kb on + strand, within EX28DRAFT_0405 at 382.656 kb on + strand, within EX28DRAFT_0405 at 382.679 kb on + strand, within EX28DRAFT_0405 at 382.783 kb on - strand, within EX28DRAFT_0405 at 382.786 kb on + strand, within EX28DRAFT_0405 at 382.817 kb on + strand at 382.817 kb on + strand at 382.818 kb on - strand at 383.007 kb on + strand at 383.059 kb on - strand at 383.059 kb on - strand at 383.166 kb on - strand, within EX28DRAFT_0406 at 383.264 kb on + strand, within EX28DRAFT_0406 at 383.264 kb on + strand, within EX28DRAFT_0406 at 383.265 kb on - strand, within EX28DRAFT_0406 at 383.265 kb on - strand, within EX28DRAFT_0406 at 383.329 kb on + strand, within EX28DRAFT_0406 at 383.346 kb on + strand, within EX28DRAFT_0406 at 383.347 kb on - strand, within EX28DRAFT_0406 at 383.347 kb on - strand, within EX28DRAFT_0406 at 383.347 kb on - strand, within EX28DRAFT_0406 at 383.351 kb on - strand, within EX28DRAFT_0406 at 383.406 kb on + strand, within EX28DRAFT_0406 at 383.461 kb on - strand, within EX28DRAFT_0406 at 383.619 kb on + strand, within EX28DRAFT_0406 at 383.620 kb on - strand, within EX28DRAFT_0406 at 383.772 kb on + strand, within EX28DRAFT_0406 at 383.772 kb on + strand, within EX28DRAFT_0406 at 383.789 kb on - strand, within EX28DRAFT_0406 at 383.858 kb on + strand, within EX28DRAFT_0406 at 383.859 kb on - strand, within EX28DRAFT_0406
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 380,770 + EX28DRAFT_0403 0.84 -0.7 380,770 + EX28DRAFT_0403 0.84 -2.4 380,770 + EX28DRAFT_0403 0.84 +0.7 380,771 - EX28DRAFT_0403 0.84 -2.4 380,771 - EX28DRAFT_0403 0.84 -1.0 380,773 - EX28DRAFT_0403 0.84 +0.2 380,954 - +1.0 380,954 - -0.0 380,957 + -2.7 380,958 - -0.9 381,808 + +0.1 381,809 - +0.1 381,811 + +1.6 381,812 - -0.6 381,922 + EX28DRAFT_0405 0.15 +0.7 382,063 + EX28DRAFT_0405 0.27 -0.6 382,128 + EX28DRAFT_0405 0.33 +0.7 382,128 + EX28DRAFT_0405 0.33 +0.8 382,141 + EX28DRAFT_0405 0.34 +0.9 382,160 + EX28DRAFT_0405 0.36 +0.5 382,161 - EX28DRAFT_0405 0.36 -0.1 382,161 - EX28DRAFT_0405 0.36 -1.0 382,161 - EX28DRAFT_0405 0.36 +1.0 382,521 - EX28DRAFT_0405 0.67 -4.8 382,601 + EX28DRAFT_0405 0.74 -2.7 382,656 + EX28DRAFT_0405 0.79 +3.1 382,679 + EX28DRAFT_0405 0.81 -0.0 382,783 - EX28DRAFT_0405 0.90 +0.7 382,786 + EX28DRAFT_0405 0.90 +0.9 382,817 + -0.4 382,817 + -0.9 382,818 - +0.2 383,007 + +0.9 383,059 - +0.8 383,059 - -0.9 383,166 - EX28DRAFT_0406 0.10 -1.5 383,264 + EX28DRAFT_0406 0.20 +0.2 383,264 + EX28DRAFT_0406 0.20 -0.3 383,265 - EX28DRAFT_0406 0.20 -3.9 383,265 - EX28DRAFT_0406 0.20 +0.6 383,329 + EX28DRAFT_0406 0.26 +0.7 383,346 + EX28DRAFT_0406 0.28 -2.0 383,347 - EX28DRAFT_0406 0.28 +1.6 383,347 - EX28DRAFT_0406 0.28 -0.7 383,347 - EX28DRAFT_0406 0.28 +0.5 383,351 - EX28DRAFT_0406 0.29 -0.7 383,406 + EX28DRAFT_0406 0.34 +0.4 383,461 - EX28DRAFT_0406 0.39 +0.0 383,619 + EX28DRAFT_0406 0.55 -0.1 383,620 - EX28DRAFT_0406 0.55 -1.9 383,772 + EX28DRAFT_0406 0.70 +0.3 383,772 + EX28DRAFT_0406 0.70 -3.3 383,789 - EX28DRAFT_0406 0.71 +0.9 383,858 + EX28DRAFT_0406 0.78 -2.5 383,859 - EX28DRAFT_0406 0.78 +0.3
Or see this region's nucleotide sequence