Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0184 and EX28DRAFT_0185 are separated by 120 nucleotides EX28DRAFT_0185 and EX28DRAFT_0186 overlap by 1 nucleotides EX28DRAFT_0186 and EX28DRAFT_0187 are separated by 73 nucleotides EX28DRAFT_0187 and EX28DRAFT_0188 are separated by 38 nucleotides
EX28DRAFT_0184: EX28DRAFT_0184 - phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6), at 133,605 to 134,318
_0184
EX28DRAFT_0185: EX28DRAFT_0185 - Predicted metalloprotease, at 134,439 to 135,308
_0185
EX28DRAFT_0186: EX28DRAFT_0186 - Predicted P-loop ATPase fused to an acetyltransferase, at 135,308 to 137,254
_0186
EX28DRAFT_0187: EX28DRAFT_0187 - Predicted esterase, at 137,328 to 138,023
_0187
EX28DRAFT_0188: EX28DRAFT_0188 - Uncharacterised protein family (UPF0370), at 138,062 to 138,259
_0188
Position (kb) 135
136
137
138 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 134.361 kb on - strand at 134.378 kb on + strand at 134.407 kb on - strand at 134.407 kb on - strand at 134.410 kb on + strand at 134.410 kb on + strand at 134.411 kb on - strand at 134.411 kb on - strand at 134.415 kb on + strand at 134.415 kb on + strand at 134.415 kb on + strand at 134.415 kb on + strand at 134.415 kb on + strand at 134.473 kb on + strand at 134.473 kb on + strand at 134.586 kb on + strand, within EX28DRAFT_0185 at 134.781 kb on + strand, within EX28DRAFT_0185 at 134.839 kb on - strand, within EX28DRAFT_0185 at 134.931 kb on + strand, within EX28DRAFT_0185 at 134.931 kb on + strand, within EX28DRAFT_0185 at 134.932 kb on - strand, within EX28DRAFT_0185 at 135.140 kb on - strand, within EX28DRAFT_0185 at 135.239 kb on + strand at 135.240 kb on - strand at 135.240 kb on - strand at 135.284 kb on + strand at 135.285 kb on - strand at 135.285 kb on - strand at 135.287 kb on + strand at 135.288 kb on - strand at 135.288 kb on - strand at 135.306 kb on + strand at 135.307 kb on - strand at 135.307 kb on - strand at 135.307 kb on - strand at 135.764 kb on - strand, within EX28DRAFT_0186 at 136.148 kb on + strand, within EX28DRAFT_0186 at 136.148 kb on + strand, within EX28DRAFT_0186 at 136.149 kb on - strand, within EX28DRAFT_0186 at 136.177 kb on + strand, within EX28DRAFT_0186 at 136.177 kb on + strand, within EX28DRAFT_0186 at 136.178 kb on - strand, within EX28DRAFT_0186 at 136.218 kb on + strand, within EX28DRAFT_0186 at 136.219 kb on - strand, within EX28DRAFT_0186 at 136.219 kb on - strand, within EX28DRAFT_0186 at 136.219 kb on - strand, within EX28DRAFT_0186 at 136.223 kb on - strand, within EX28DRAFT_0186 at 136.384 kb on - strand, within EX28DRAFT_0186 at 136.384 kb on - strand, within EX28DRAFT_0186 at 136.385 kb on + strand, within EX28DRAFT_0186 at 136.665 kb on + strand, within EX28DRAFT_0186 at 136.665 kb on + strand, within EX28DRAFT_0186 at 136.665 kb on + strand, within EX28DRAFT_0186 at 136.753 kb on + strand, within EX28DRAFT_0186 at 136.839 kb on - strand, within EX28DRAFT_0186 at 137.264 kb on + strand at 137.312 kb on + strand at 137.312 kb on + strand at 137.312 kb on + strand at 137.313 kb on - strand at 137.380 kb on + strand at 137.402 kb on - strand, within EX28DRAFT_0187 at 137.421 kb on - strand, within EX28DRAFT_0187 at 137.913 kb on - strand, within EX28DRAFT_0187 at 137.962 kb on - strand at 137.962 kb on - strand at 138.017 kb on + strand at 138.063 kb on + strand at 138.241 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 134,361 - +2.4 134,378 + -0.9 134,407 - -0.3 134,407 - +0.4 134,410 + +0.3 134,410 + +0.8 134,411 - -1.2 134,411 - -0.0 134,415 + -0.1 134,415 + -0.2 134,415 + -2.9 134,415 + +0.3 134,415 + -0.6 134,473 + -0.2 134,473 + +1.2 134,586 + EX28DRAFT_0185 0.17 +0.7 134,781 + EX28DRAFT_0185 0.39 -3.0 134,839 - EX28DRAFT_0185 0.46 +0.1 134,931 + EX28DRAFT_0185 0.57 +0.2 134,931 + EX28DRAFT_0185 0.57 +2.1 134,932 - EX28DRAFT_0185 0.57 +1.2 135,140 - EX28DRAFT_0185 0.81 +0.8 135,239 + -0.7 135,240 - +0.2 135,240 - -1.3 135,284 + +1.8 135,285 - +0.0 135,285 - +0.4 135,287 + -0.2 135,288 - +1.4 135,288 - +0.5 135,306 + -2.4 135,307 - -0.5 135,307 - -0.3 135,307 - +0.3 135,764 - EX28DRAFT_0186 0.23 +0.2 136,148 + EX28DRAFT_0186 0.43 +1.5 136,148 + EX28DRAFT_0186 0.43 +0.3 136,149 - EX28DRAFT_0186 0.43 +1.2 136,177 + EX28DRAFT_0186 0.45 +0.8 136,177 + EX28DRAFT_0186 0.45 +0.1 136,178 - EX28DRAFT_0186 0.45 -1.6 136,218 + EX28DRAFT_0186 0.47 +0.8 136,219 - EX28DRAFT_0186 0.47 +0.2 136,219 - EX28DRAFT_0186 0.47 +0.4 136,219 - EX28DRAFT_0186 0.47 +1.3 136,223 - EX28DRAFT_0186 0.47 -2.4 136,384 - EX28DRAFT_0186 0.55 +1.0 136,384 - EX28DRAFT_0186 0.55 -0.5 136,385 + EX28DRAFT_0186 0.55 -1.3 136,665 + EX28DRAFT_0186 0.70 +0.7 136,665 + EX28DRAFT_0186 0.70 -0.6 136,665 + EX28DRAFT_0186 0.70 +0.4 136,753 + EX28DRAFT_0186 0.74 +1.4 136,839 - EX28DRAFT_0186 0.79 +0.8 137,264 + -3.3 137,312 + +0.1 137,312 + +0.7 137,312 + -0.2 137,313 - +0.5 137,380 + -2.4 137,402 - EX28DRAFT_0187 0.11 +0.1 137,421 - EX28DRAFT_0187 0.13 -0.1 137,913 - EX28DRAFT_0187 0.84 +0.4 137,962 - +0.3 137,962 - +0.3 138,017 + -2.4 138,063 + -1.6 138,241 - -1.5
Or see this region's nucleotide sequence