Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0144 and EX28DRAFT_0145 are separated by 656 nucleotides EX28DRAFT_0145 and EX28DRAFT_0146 are separated by 82 nucleotides
EX28DRAFT_0144: EX28DRAFT_0144 - Fels-1 Prophage Protein-like, at 88,557 to 88,919
_0144
EX28DRAFT_0145: EX28DRAFT_0145 - Protein of unknown function (DUF1641), at 89,576 to 89,893
_0145
EX28DRAFT_0146: EX28DRAFT_0146 - transcriptional regulator, RpiR family, at 89,976 to 90,809
_0146
Position (kb) 89
90 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 88.677 kb on + strand, within EX28DRAFT_0144 at 88.681 kb on + strand, within EX28DRAFT_0144 at 88.681 kb on + strand, within EX28DRAFT_0144 at 88.681 kb on + strand, within EX28DRAFT_0144 at 88.681 kb on + strand, within EX28DRAFT_0144 at 88.682 kb on - strand, within EX28DRAFT_0144 at 88.682 kb on - strand, within EX28DRAFT_0144 at 88.725 kb on + strand, within EX28DRAFT_0144 at 88.725 kb on + strand, within EX28DRAFT_0144 at 88.725 kb on + strand, within EX28DRAFT_0144 at 88.726 kb on - strand, within EX28DRAFT_0144 at 88.848 kb on + strand, within EX28DRAFT_0144 at 88.849 kb on - strand, within EX28DRAFT_0144 at 88.852 kb on - strand, within EX28DRAFT_0144 at 88.886 kb on + strand at 88.951 kb on - strand at 88.951 kb on - strand at 88.965 kb on + strand at 88.965 kb on + strand at 88.966 kb on - strand at 88.966 kb on - strand at 88.991 kb on + strand at 89.017 kb on - strand at 89.035 kb on + strand at 89.036 kb on - strand at 89.047 kb on + strand at 89.047 kb on + strand at 89.069 kb on - strand at 89.079 kb on - strand at 89.271 kb on + strand at 89.271 kb on + strand at 89.288 kb on + strand at 89.289 kb on - strand at 89.293 kb on - strand at 89.293 kb on - strand at 89.347 kb on + strand at 89.369 kb on + strand at 89.398 kb on + strand at 89.399 kb on - strand at 89.435 kb on + strand at 89.436 kb on - strand at 89.523 kb on - strand at 89.528 kb on + strand at 89.546 kb on + strand at 89.546 kb on + strand at 89.547 kb on - strand at 89.548 kb on + strand at 89.549 kb on - strand at 89.741 kb on - strand, within EX28DRAFT_0145 at 89.840 kb on - strand, within EX28DRAFT_0145 at 89.925 kb on + strand at 89.925 kb on + strand at 89.943 kb on + strand at 89.943 kb on + strand at 89.944 kb on - strand at 89.944 kb on - strand at 89.961 kb on - strand at 89.981 kb on + strand at 89.981 kb on + strand at 90.320 kb on - strand, within EX28DRAFT_0146 at 90.329 kb on - strand, within EX28DRAFT_0146 at 90.341 kb on + strand, within EX28DRAFT_0146 at 90.367 kb on + strand, within EX28DRAFT_0146 at 90.367 kb on + strand, within EX28DRAFT_0146 at 90.367 kb on + strand, within EX28DRAFT_0146 at 90.368 kb on - strand, within EX28DRAFT_0146 at 90.547 kb on + strand, within EX28DRAFT_0146 at 90.832 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 88,677 + EX28DRAFT_0144 0.33 -0.4 88,681 + EX28DRAFT_0144 0.34 +1.4 88,681 + EX28DRAFT_0144 0.34 +0.4 88,681 + EX28DRAFT_0144 0.34 +0.2 88,681 + EX28DRAFT_0144 0.34 +1.6 88,682 - EX28DRAFT_0144 0.34 +0.0 88,682 - EX28DRAFT_0144 0.34 +0.1 88,725 + EX28DRAFT_0144 0.46 -0.1 88,725 + EX28DRAFT_0144 0.46 +0.1 88,725 + EX28DRAFT_0144 0.46 -0.1 88,726 - EX28DRAFT_0144 0.47 -0.7 88,848 + EX28DRAFT_0144 0.80 +0.0 88,849 - EX28DRAFT_0144 0.80 +0.0 88,852 - EX28DRAFT_0144 0.81 +0.2 88,886 + +0.4 88,951 - +0.5 88,951 - +0.4 88,965 + -4.1 88,965 + -0.1 88,966 - +0.5 88,966 - -0.4 88,991 + -1.4 89,017 - -2.2 89,035 + -2.7 89,036 - -1.3 89,047 + +0.7 89,047 + +0.2 89,069 - -0.4 89,079 - +0.1 89,271 + -0.1 89,271 + -1.3 89,288 + +0.7 89,289 - +0.8 89,293 - +0.9 89,293 - -0.4 89,347 + +0.0 89,369 + -0.1 89,398 + -2.9 89,399 - +0.4 89,435 + +0.4 89,436 - +0.3 89,523 - -0.3 89,528 + -0.4 89,546 + -3.0 89,546 + +0.5 89,547 - +0.0 89,548 + -0.0 89,549 - +0.2 89,741 - EX28DRAFT_0145 0.52 -0.9 89,840 - EX28DRAFT_0145 0.83 -3.9 89,925 + -0.6 89,925 + -0.3 89,943 + +0.9 89,943 + -0.6 89,944 - +1.1 89,944 - -0.2 89,961 - -1.5 89,981 + -1.6 89,981 + -0.2 90,320 - EX28DRAFT_0146 0.41 +1.1 90,329 - EX28DRAFT_0146 0.42 +0.2 90,341 + EX28DRAFT_0146 0.44 -1.8 90,367 + EX28DRAFT_0146 0.47 -1.4 90,367 + EX28DRAFT_0146 0.47 -0.8 90,367 + EX28DRAFT_0146 0.47 +0.4 90,368 - EX28DRAFT_0146 0.47 -0.1 90,547 + EX28DRAFT_0146 0.68 -2.4 90,832 - +0.3
Or see this region's nucleotide sequence