Experiment: without_iron1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS05225 and AMB_RS05230 are separated by 6 nucleotides AMB_RS05230 and AMB_RS23960 overlap by 107 nucleotides AMB_RS23960 and AMB_RS05235 are separated by 167 nucleotides AMB_RS05235 and AMB_RS05240 are separated by 8 nucleotides AMB_RS05240 and AMB_RS05245 are separated by 35 nucleotides
AMB_RS05225: AMB_RS05225 - hypothetical protein, at 1,080,934 to 1,081,887
_RS05225
AMB_RS05230: AMB_RS05230 - protein kinase family protein, at 1,081,894 to 1,082,859
_RS05230
AMB_RS23960: AMB_RS23960 - hypothetical protein, at 1,082,753 to 1,083,085
_RS23960
AMB_RS05235: AMB_RS05235 - hypothetical protein, at 1,083,253 to 1,083,687
_RS05235
AMB_RS05240: AMB_RS05240 - ferrous iron transport protein A, at 1,083,696 to 1,084,016
_RS05240
AMB_RS05245: AMB_RS05245 - ferrous iron transport protein B, at 1,084,052 to 1,086,166
_RS05245
Position (kb)
1082
1083
1084 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1081.918 kb on + strand at 1081.918 kb on + strand at 1081.919 kb on - strand at 1081.919 kb on - strand at 1081.919 kb on - strand at 1081.919 kb on - strand at 1081.919 kb on - strand at 1082.107 kb on + strand, within AMB_RS05230 at 1082.107 kb on + strand, within AMB_RS05230 at 1082.122 kb on - strand, within AMB_RS05230 at 1082.122 kb on - strand, within AMB_RS05230 at 1082.122 kb on - strand, within AMB_RS05230 at 1082.149 kb on + strand, within AMB_RS05230 at 1082.149 kb on + strand, within AMB_RS05230 at 1082.150 kb on - strand, within AMB_RS05230 at 1082.150 kb on - strand, within AMB_RS05230 at 1082.150 kb on - strand, within AMB_RS05230 at 1082.218 kb on + strand, within AMB_RS05230 at 1082.432 kb on - strand, within AMB_RS05230 at 1082.432 kb on - strand, within AMB_RS05230 at 1082.432 kb on - strand, within AMB_RS05230 at 1082.443 kb on + strand, within AMB_RS05230 at 1082.443 kb on + strand, within AMB_RS05230 at 1082.443 kb on + strand, within AMB_RS05230 at 1082.443 kb on + strand, within AMB_RS05230 at 1082.444 kb on - strand, within AMB_RS05230 at 1082.444 kb on - strand, within AMB_RS05230 at 1082.444 kb on - strand, within AMB_RS05230 at 1082.754 kb on + strand at 1082.754 kb on + strand at 1082.754 kb on + strand at 1082.755 kb on - strand at 1082.755 kb on - strand at 1082.755 kb on - strand at 1082.755 kb on - strand at 1082.784 kb on - strand at 1082.784 kb on - strand at 1082.784 kb on - strand at 1082.910 kb on + strand, within AMB_RS23960 at 1082.999 kb on + strand, within AMB_RS23960 at 1083.102 kb on + strand at 1083.143 kb on + strand at 1083.145 kb on + strand at 1083.145 kb on + strand at 1083.146 kb on - strand at 1083.146 kb on - strand at 1083.146 kb on - strand at 1083.146 kb on - strand at 1083.204 kb on + strand at 1083.204 kb on + strand at 1083.205 kb on - strand at 1083.252 kb on - strand at 1083.330 kb on - strand, within AMB_RS05235 at 1083.611 kb on + strand, within AMB_RS05235 at 1083.612 kb on - strand, within AMB_RS05235 at 1083.632 kb on - strand, within AMB_RS05235 at 1083.743 kb on - strand, within AMB_RS05240 at 1083.743 kb on - strand, within AMB_RS05240 at 1083.743 kb on - strand, within AMB_RS05240 at 1083.743 kb on - strand, within AMB_RS05240 at 1083.743 kb on - strand, within AMB_RS05240 at 1083.743 kb on - strand, within AMB_RS05240 at 1083.886 kb on + strand, within AMB_RS05240 at 1083.928 kb on + strand, within AMB_RS05240 at 1083.929 kb on - strand, within AMB_RS05240 at 1083.946 kb on - strand, within AMB_RS05240 at 1083.961 kb on - strand, within AMB_RS05240 at 1084.014 kb on + strand at 1084.015 kb on - strand at 1084.015 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction without_iron1 remove 1,081,918 + -0.5 1,081,918 + -0.7 1,081,919 - +0.4 1,081,919 - -2.4 1,081,919 - -0.9 1,081,919 - -0.2 1,081,919 - +0.8 1,082,107 + AMB_RS05230 0.22 -1.1 1,082,107 + AMB_RS05230 0.22 -0.8 1,082,122 - AMB_RS05230 0.24 -0.3 1,082,122 - AMB_RS05230 0.24 +0.0 1,082,122 - AMB_RS05230 0.24 -0.9 1,082,149 + AMB_RS05230 0.26 -0.3 1,082,149 + AMB_RS05230 0.26 -0.1 1,082,150 - AMB_RS05230 0.27 -0.9 1,082,150 - AMB_RS05230 0.27 +0.0 1,082,150 - AMB_RS05230 0.27 +0.2 1,082,218 + AMB_RS05230 0.34 -0.4 1,082,432 - AMB_RS05230 0.56 -0.6 1,082,432 - AMB_RS05230 0.56 +0.2 1,082,432 - AMB_RS05230 0.56 +0.8 1,082,443 + AMB_RS05230 0.57 -0.5 1,082,443 + AMB_RS05230 0.57 -0.7 1,082,443 + AMB_RS05230 0.57 -0.7 1,082,443 + AMB_RS05230 0.57 -0.3 1,082,444 - AMB_RS05230 0.57 -0.0 1,082,444 - AMB_RS05230 0.57 -0.4 1,082,444 - AMB_RS05230 0.57 -0.3 1,082,754 + -0.3 1,082,754 + -2.3 1,082,754 + +0.6 1,082,755 - -0.1 1,082,755 - -0.5 1,082,755 - +0.2 1,082,755 - -0.2 1,082,784 - +0.2 1,082,784 - -0.4 1,082,784 - -0.2 1,082,910 + AMB_RS23960 0.47 -0.4 1,082,999 + AMB_RS23960 0.74 -1.1 1,083,102 + +0.8 1,083,143 + -0.6 1,083,145 + -0.7 1,083,145 + +0.2 1,083,146 - -1.0 1,083,146 - -1.8 1,083,146 - -0.5 1,083,146 - +0.5 1,083,204 + -0.1 1,083,204 + -0.4 1,083,205 - +0.3 1,083,252 - -2.4 1,083,330 - AMB_RS05235 0.18 -1.7 1,083,611 + AMB_RS05235 0.82 +0.4 1,083,612 - AMB_RS05235 0.83 +0.2 1,083,632 - AMB_RS05235 0.87 -0.1 1,083,743 - AMB_RS05240 0.15 +1.8 1,083,743 - AMB_RS05240 0.15 +0.7 1,083,743 - AMB_RS05240 0.15 +0.5 1,083,743 - AMB_RS05240 0.15 -0.4 1,083,743 - AMB_RS05240 0.15 -0.5 1,083,743 - AMB_RS05240 0.15 +0.3 1,083,886 + AMB_RS05240 0.59 +1.1 1,083,928 + AMB_RS05240 0.72 +0.2 1,083,929 - AMB_RS05240 0.73 +0.2 1,083,946 - AMB_RS05240 0.78 -0.2 1,083,961 - AMB_RS05240 0.83 -0.1 1,084,014 + +0.0 1,084,015 - +0.2 1,084,015 - +0.5
Or see this region's nucleotide sequence