Experiment: without_iron1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS20530 and AMB_RS20535 are separated by 137 nucleotides AMB_RS20535 and AMB_RS20540 are separated by 79 nucleotides AMB_RS20540 and AMB_RS20545 are separated by 6 nucleotides AMB_RS20545 and AMB_RS20550 overlap by 11 nucleotides
AMB_RS20530: AMB_RS20530 - ATP-dependent helicase, at 4,461,761 to 4,463,521
_RS20530
AMB_RS20535: AMB_RS20535 - signal recognition particle protein, at 4,463,659 to 4,465,023
_RS20535
AMB_RS20540: AMB_RS20540 - 30S ribosomal protein S16, at 4,465,103 to 4,465,474
_RS20540
AMB_RS20545: AMB_RS20545 - ribosome maturation factor RimM, at 4,465,481 to 4,466,011
_RS20545
AMB_RS20550: AMB_RS20550 - tRNA (guanine-N(1)-)-methyltransferase, at 4,466,001 to 4,466,717
_RS20550
Position (kb)
4463
4464
4465
4466 Strain fitness (log2 ratio)
-1
0
1
2 at 4463.027 kb on + strand, within AMB_RS20530 at 4463.028 kb on - strand, within AMB_RS20530 at 4463.028 kb on - strand, within AMB_RS20530 at 4463.028 kb on - strand, within AMB_RS20530 at 4463.028 kb on - strand, within AMB_RS20530 at 4463.028 kb on - strand, within AMB_RS20530 at 4463.028 kb on - strand, within AMB_RS20530 at 4463.249 kb on - strand, within AMB_RS20530 at 4463.249 kb on - strand, within AMB_RS20530 at 4463.249 kb on - strand, within AMB_RS20530 at 4463.639 kb on + strand at 4465.970 kb on + strand at 4465.970 kb on + strand at 4465.970 kb on + strand at 4465.970 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction without_iron1 remove 4,463,027 + AMB_RS20530 0.72 -0.3 4,463,028 - AMB_RS20530 0.72 -0.2 4,463,028 - AMB_RS20530 0.72 +0.7 4,463,028 - AMB_RS20530 0.72 -0.7 4,463,028 - AMB_RS20530 0.72 -0.5 4,463,028 - AMB_RS20530 0.72 +0.3 4,463,028 - AMB_RS20530 0.72 -0.1 4,463,249 - AMB_RS20530 0.84 -0.0 4,463,249 - AMB_RS20530 0.84 +0.3 4,463,249 - AMB_RS20530 0.84 -0.4 4,463,639 + +0.3 4,465,970 + +0.1 4,465,970 + +1.9 4,465,970 + -0.2 4,465,970 + -0.7
Or see this region's nucleotide sequence