Experiment: without_iron1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS12345 and AMB_RS12350 are separated by 248 nucleotides AMB_RS12350 and AMB_RS12355 overlap by 1 nucleotides AMB_RS12355 and AMB_RS12360 overlap by 4 nucleotides AMB_RS12360 and AMB_RS12365 overlap by 4 nucleotides
AMB_RS12345: AMB_RS12345 - aldo/keto reductase, at 2,632,919 to 2,633,782
_RS12345
AMB_RS12350: AMB_RS12350 - ABC transporter ATP-binding protein, at 2,634,031 to 2,634,735
_RS12350
AMB_RS12355: AMB_RS12355 - ABC transporter ATP-binding protein, at 2,634,735 to 2,635,466
_RS12355
AMB_RS12360: AMB_RS12360 - branched-chain amino acid ABC transporter permease, at 2,635,463 to 2,636,455
_RS12360
AMB_RS12365: AMB_RS12365 - branched-chain amino acid ABC transporter permease, at 2,636,452 to 2,637,318
_RS12365
Position (kb)
2634
2635
2636 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2633.811 kb on + strand at 2633.843 kb on + strand at 2633.932 kb on - strand at 2634.033 kb on - strand at 2634.033 kb on - strand at 2634.044 kb on + strand at 2634.044 kb on + strand at 2634.044 kb on + strand at 2634.045 kb on - strand at 2634.118 kb on + strand, within AMB_RS12350 at 2634.138 kb on + strand, within AMB_RS12350 at 2634.139 kb on - strand, within AMB_RS12350 at 2634.139 kb on - strand, within AMB_RS12350 at 2634.139 kb on - strand, within AMB_RS12350 at 2634.758 kb on - strand at 2634.758 kb on - strand at 2634.911 kb on + strand, within AMB_RS12355 at 2635.573 kb on - strand, within AMB_RS12360 at 2635.573 kb on - strand, within AMB_RS12360 at 2635.767 kb on + strand, within AMB_RS12360 at 2635.767 kb on + strand, within AMB_RS12360 at 2635.767 kb on + strand, within AMB_RS12360 at 2635.768 kb on - strand, within AMB_RS12360 at 2635.768 kb on - strand, within AMB_RS12360 at 2635.768 kb on - strand, within AMB_RS12360 at 2635.770 kb on + strand, within AMB_RS12360 at 2635.770 kb on + strand, within AMB_RS12360 at 2635.771 kb on - strand, within AMB_RS12360 at 2635.771 kb on - strand, within AMB_RS12360 at 2635.771 kb on - strand, within AMB_RS12360 at 2635.827 kb on + strand, within AMB_RS12360 at 2635.828 kb on - strand, within AMB_RS12360 at 2635.828 kb on - strand, within AMB_RS12360 at 2635.828 kb on - strand, within AMB_RS12360 at 2635.902 kb on + strand, within AMB_RS12360 at 2635.971 kb on + strand, within AMB_RS12360 at 2635.972 kb on - strand, within AMB_RS12360 at 2635.974 kb on + strand, within AMB_RS12360 at 2635.974 kb on + strand, within AMB_RS12360 at 2635.974 kb on + strand, within AMB_RS12360 at 2635.974 kb on + strand, within AMB_RS12360 at 2635.975 kb on - strand, within AMB_RS12360 at 2635.975 kb on - strand, within AMB_RS12360 at 2636.127 kb on + strand, within AMB_RS12360 at 2636.127 kb on + strand, within AMB_RS12360 at 2636.128 kb on - strand, within AMB_RS12360 at 2636.257 kb on + strand, within AMB_RS12360 at 2636.259 kb on + strand, within AMB_RS12360 at 2636.259 kb on + strand, within AMB_RS12360 at 2636.260 kb on - strand, within AMB_RS12360 at 2636.277 kb on + strand, within AMB_RS12360 at 2636.277 kb on + strand, within AMB_RS12360 at 2636.277 kb on + strand, within AMB_RS12360 at 2636.278 kb on - strand, within AMB_RS12360 at 2636.278 kb on - strand, within AMB_RS12360 at 2636.278 kb on - strand, within AMB_RS12360 at 2636.343 kb on + strand, within AMB_RS12360 at 2636.344 kb on - strand, within AMB_RS12360 at 2636.344 kb on - strand, within AMB_RS12360 at 2636.344 kb on - strand, within AMB_RS12360 at 2636.352 kb on + strand, within AMB_RS12360 at 2636.352 kb on + strand, within AMB_RS12360 at 2636.352 kb on + strand, within AMB_RS12360 at 2636.353 kb on - strand, within AMB_RS12360 at 2636.353 kb on - strand, within AMB_RS12360 at 2636.353 kb on - strand, within AMB_RS12360 at 2636.382 kb on + strand at 2636.382 kb on + strand at 2636.382 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction without_iron1 remove 2,633,811 + -0.1 2,633,843 + -1.5 2,633,932 - -0.9 2,634,033 - -1.1 2,634,033 - -0.9 2,634,044 + -0.4 2,634,044 + -0.4 2,634,044 + -3.3 2,634,045 - -0.7 2,634,118 + AMB_RS12350 0.12 -0.4 2,634,138 + AMB_RS12350 0.15 -0.9 2,634,139 - AMB_RS12350 0.15 -0.1 2,634,139 - AMB_RS12350 0.15 -0.9 2,634,139 - AMB_RS12350 0.15 -0.5 2,634,758 - -0.1 2,634,758 - -0.7 2,634,911 + AMB_RS12355 0.24 +0.3 2,635,573 - AMB_RS12360 0.11 -1.2 2,635,573 - AMB_RS12360 0.11 +0.6 2,635,767 + AMB_RS12360 0.31 -0.1 2,635,767 + AMB_RS12360 0.31 -0.9 2,635,767 + AMB_RS12360 0.31 +0.5 2,635,768 - AMB_RS12360 0.31 +0.2 2,635,768 - AMB_RS12360 0.31 -0.5 2,635,768 - AMB_RS12360 0.31 -0.3 2,635,770 + AMB_RS12360 0.31 +0.5 2,635,770 + AMB_RS12360 0.31 +0.1 2,635,771 - AMB_RS12360 0.31 -0.6 2,635,771 - AMB_RS12360 0.31 -1.0 2,635,771 - AMB_RS12360 0.31 +0.5 2,635,827 + AMB_RS12360 0.37 -0.6 2,635,828 - AMB_RS12360 0.37 -0.4 2,635,828 - AMB_RS12360 0.37 +0.3 2,635,828 - AMB_RS12360 0.37 -0.2 2,635,902 + AMB_RS12360 0.44 -0.6 2,635,971 + AMB_RS12360 0.51 -0.3 2,635,972 - AMB_RS12360 0.51 +0.7 2,635,974 + AMB_RS12360 0.51 +0.5 2,635,974 + AMB_RS12360 0.51 +1.0 2,635,974 + AMB_RS12360 0.51 -0.1 2,635,974 + AMB_RS12360 0.51 -0.7 2,635,975 - AMB_RS12360 0.52 -0.4 2,635,975 - AMB_RS12360 0.52 -0.3 2,636,127 + AMB_RS12360 0.67 +0.7 2,636,127 + AMB_RS12360 0.67 -0.7 2,636,128 - AMB_RS12360 0.67 +0.5 2,636,257 + AMB_RS12360 0.80 -0.6 2,636,259 + AMB_RS12360 0.80 -0.1 2,636,259 + AMB_RS12360 0.80 +0.6 2,636,260 - AMB_RS12360 0.80 +0.1 2,636,277 + AMB_RS12360 0.82 +1.6 2,636,277 + AMB_RS12360 0.82 -0.9 2,636,277 + AMB_RS12360 0.82 -0.0 2,636,278 - AMB_RS12360 0.82 +0.4 2,636,278 - AMB_RS12360 0.82 -0.3 2,636,278 - AMB_RS12360 0.82 +0.3 2,636,343 + AMB_RS12360 0.89 -0.2 2,636,344 - AMB_RS12360 0.89 +0.2 2,636,344 - AMB_RS12360 0.89 +0.1 2,636,344 - AMB_RS12360 0.89 -0.1 2,636,352 + AMB_RS12360 0.90 +0.1 2,636,352 + AMB_RS12360 0.90 +0.2 2,636,352 + AMB_RS12360 0.90 -0.9 2,636,353 - AMB_RS12360 0.90 -0.1 2,636,353 - AMB_RS12360 0.90 -1.0 2,636,353 - AMB_RS12360 0.90 +0.0 2,636,382 + -0.3 2,636,382 + +0.1 2,636,382 + +0.3
Or see this region's nucleotide sequence