Experiment: without_iron1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS04280 and AMB_RS04285 are separated by 130 nucleotides AMB_RS04285 and AMB_RS04290 overlap by 71 nucleotides AMB_RS04290 and AMB_RS04295 are separated by 137 nucleotides
AMB_RS04280: AMB_RS04280 - polyhydroxyalkanoate synthesis repressor PhaR, at 899,453 to 900,016
_RS04280
AMB_RS04285: AMB_RS04285 - hypothetical protein, at 900,147 to 900,596
_RS04285
AMB_RS04290: AMB_RS04290 - poly-beta-hydroxybutyrate polymerase, at 900,526 to 901,632
_RS04290
AMB_RS04295: AMB_RS04295 - acetyl-CoA C-acetyltransferase, at 901,770 to 902,942
_RS04295
Position (kb)
900
901
902 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.553 kb on + strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.554 kb on - strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.659 kb on + strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.660 kb on - strand, within AMB_RS04280 at 899.661 kb on + strand, within AMB_RS04280 at 899.661 kb on + strand, within AMB_RS04280 at 899.661 kb on + strand, within AMB_RS04280 at 899.662 kb on - strand, within AMB_RS04280 at 899.662 kb on - strand, within AMB_RS04280 at 899.662 kb on - strand, within AMB_RS04280 at 899.662 kb on - strand, within AMB_RS04280 at 899.748 kb on + strand, within AMB_RS04280 at 899.848 kb on + strand, within AMB_RS04280 at 899.929 kb on - strand, within AMB_RS04280 at 899.968 kb on - strand at 900.073 kb on + strand at 900.073 kb on + strand at 900.074 kb on - strand at 900.074 kb on - strand at 900.076 kb on + strand at 900.087 kb on + strand at 900.087 kb on + strand at 900.087 kb on + strand at 900.146 kb on + strand at 900.171 kb on + strand at 900.171 kb on + strand at 901.630 kb on + strand at 901.630 kb on + strand at 901.630 kb on + strand at 901.630 kb on + strand at 901.630 kb on + strand at 901.630 kb on + strand at 901.630 kb on + strand at 901.742 kb on + strand at 901.743 kb on - strand at 901.744 kb on + strand at 901.744 kb on + strand at 901.744 kb on + strand at 901.745 kb on - strand at 902.145 kb on + strand, within AMB_RS04295
Per-strain Table
Position Strand Gene LocusTag Fraction without_iron1 remove 899,553 + AMB_RS04280 0.18 -0.4 899,553 + AMB_RS04280 0.18 -1.2 899,553 + AMB_RS04280 0.18 -2.3 899,553 + AMB_RS04280 0.18 -0.3 899,553 + AMB_RS04280 0.18 -0.6 899,553 + AMB_RS04280 0.18 -0.6 899,553 + AMB_RS04280 0.18 -1.0 899,553 + AMB_RS04280 0.18 -0.5 899,553 + AMB_RS04280 0.18 -0.9 899,553 + AMB_RS04280 0.18 -0.9 899,554 - AMB_RS04280 0.18 -0.7 899,554 - AMB_RS04280 0.18 -0.6 899,554 - AMB_RS04280 0.18 -0.9 899,554 - AMB_RS04280 0.18 +0.3 899,554 - AMB_RS04280 0.18 -2.9 899,554 - AMB_RS04280 0.18 -0.4 899,554 - AMB_RS04280 0.18 -0.4 899,554 - AMB_RS04280 0.18 -0.2 899,554 - AMB_RS04280 0.18 -0.5 899,554 - AMB_RS04280 0.18 -1.2 899,554 - AMB_RS04280 0.18 +0.5 899,554 - AMB_RS04280 0.18 -0.6 899,554 - AMB_RS04280 0.18 -0.6 899,554 - AMB_RS04280 0.18 -1.2 899,554 - AMB_RS04280 0.18 -1.0 899,554 - AMB_RS04280 0.18 -1.1 899,554 - AMB_RS04280 0.18 -0.2 899,659 + AMB_RS04280 0.37 -0.0 899,659 + AMB_RS04280 0.37 -1.3 899,659 + AMB_RS04280 0.37 -0.2 899,659 + AMB_RS04280 0.37 -0.3 899,659 + AMB_RS04280 0.37 -0.1 899,659 + AMB_RS04280 0.37 -0.8 899,659 + AMB_RS04280 0.37 +0.1 899,659 + AMB_RS04280 0.37 -0.3 899,659 + AMB_RS04280 0.37 -2.1 899,659 + AMB_RS04280 0.37 -0.2 899,659 + AMB_RS04280 0.37 +0.3 899,659 + AMB_RS04280 0.37 -1.9 899,659 + AMB_RS04280 0.37 -0.1 899,660 - AMB_RS04280 0.37 -1.7 899,660 - AMB_RS04280 0.37 -2.9 899,660 - AMB_RS04280 0.37 -0.4 899,660 - AMB_RS04280 0.37 -1.7 899,660 - AMB_RS04280 0.37 -1.7 899,660 - AMB_RS04280 0.37 -2.6 899,660 - AMB_RS04280 0.37 -1.1 899,661 + AMB_RS04280 0.37 +0.9 899,661 + AMB_RS04280 0.37 -0.7 899,661 + AMB_RS04280 0.37 -2.8 899,662 - AMB_RS04280 0.37 -0.9 899,662 - AMB_RS04280 0.37 +1.3 899,662 - AMB_RS04280 0.37 -2.5 899,662 - AMB_RS04280 0.37 -0.7 899,748 + AMB_RS04280 0.52 +0.3 899,848 + AMB_RS04280 0.70 -1.7 899,929 - AMB_RS04280 0.84 +0.3 899,968 - -2.3 900,073 + -2.1 900,073 + -0.3 900,074 - -0.9 900,074 - -0.4 900,076 + -1.3 900,087 + -1.8 900,087 + -1.4 900,087 + -2.1 900,146 + -0.1 900,171 + -0.1 900,171 + -0.1 901,630 + +0.3 901,630 + -0.4 901,630 + -1.2 901,630 + -2.5 901,630 + -0.6 901,630 + -1.4 901,630 + -1.1 901,742 + -0.1 901,743 - -0.7 901,744 + -0.4 901,744 + +0.0 901,744 + -0.4 901,745 - -0.5 902,145 + AMB_RS04295 0.32 -0.3
Or see this region's nucleotide sequence