Strain Fitness in Escherichia coli BW25113 around b2608

Experiment: L-Serine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrplS and trmD are separated by 41 nucleotidestrmD and yfjA are separated by 30 nucleotidesyfjA and rpsP are separated by 18 nucleotidesrpsP and ffh are separated by 248 nucleotides b2606: rplS - 50S ribosomal protein L19 (NCBI), at 2,742,205 to 2,742,552 rplS b2607: trmD - tRNA (guanine-N(1)-)-methyltransferase (NCBI), at 2,742,594 to 2,743,361 trmD b2608: yfjA - orf, hypothetical protein (VIMSS), at 2,743,392 to 2,743,940 yfjA b2609: rpsP - 30S ribosomal protein S16 (NCBI), at 2,743,959 to 2,744,207 rpsP b2610: ffh - Signal Recognition Particle (SRP) component with 4.5S RNA (ffs) (NCBI), at 2,744,456 to 2,745,817 ffh Position (kb) 2743 2744Strain fitness (log2 ratio) -1 0 1at 2742.626 kb on - strandat 2743.986 kb on - strand, within rpsPat 2743.986 kb on - strand, within rpsPat 2744.337 kb on - strandat 2744.337 kb on - strandat 2744.358 kb on - strandat 2744.358 kb on - strandat 2744.400 kb on + strandat 2744.417 kb on + strandat 2744.417 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Serine (N)
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2,742,626 - +0.3
2,743,986 - rpsP b2609 0.11 -1.7
2,743,986 - rpsP b2609 0.11 -1.7
2,744,337 - +0.0
2,744,337 - +0.3
2,744,358 - +1.0
2,744,358 - +0.1
2,744,400 + -0.1
2,744,417 + +0.3
2,744,417 - -1.4

Or see this region's nucleotide sequence