Strain Fitness in Magnetospirillum magneticum AMB-1 around AMB_RS12400

Experiment: 30_nonmag_3

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAMB_RS12390 and AMB_RS12395 overlap by 4 nucleotidesAMB_RS12395 and AMB_RS12400 are separated by 20 nucleotidesAMB_RS12400 and AMB_RS12405 are separated by 91 nucleotides AMB_RS12390: AMB_RS12390 - transcription-repair coupling factor, at 2,642,039 to 2,645,518 _RS12390 AMB_RS12395: AMB_RS12395 - succinate dehydrogenase assembly factor 2, at 2,645,515 to 2,645,832 _RS12395 AMB_RS12400: AMB_RS12400 - ATP-dependent DNA helicase RecG, at 2,645,853 to 2,647,934 _RS12400 AMB_RS12405: AMB_RS12405 - UPF0016 domain-containing protein, at 2,648,026 to 2,648,607 _RS12405 Position (kb) 2645 2646 2647 2648Strain fitness (log2 ratio) -3 -2 -1 0 1at 2644.909 kb on + strand, within AMB_RS12390at 2644.910 kb on - strand, within AMB_RS12390at 2644.910 kb on - strand, within AMB_RS12390at 2644.998 kb on + strand, within AMB_RS12390at 2645.028 kb on + strand, within AMB_RS12390at 2645.064 kb on + strand, within AMB_RS12390at 2645.286 kb on + strandat 2645.287 kb on - strandat 2645.394 kb on - strandat 2645.785 kb on - strand, within AMB_RS12395at 2645.785 kb on - strand, within AMB_RS12395at 2645.785 kb on - strand, within AMB_RS12395at 2645.785 kb on - strand, within AMB_RS12395at 2645.815 kb on + strandat 2645.816 kb on - strandat 2645.821 kb on - strandat 2645.822 kb on + strandat 2645.823 kb on - strandat 2645.823 kb on - strandat 2645.823 kb on - strandat 2647.921 kb on + strandat 2647.974 kb on + strandat 2647.974 kb on + strandat 2647.975 kb on - strandat 2647.975 kb on - strandat 2647.975 kb on - strandat 2648.100 kb on + strand, within AMB_RS12405at 2648.100 kb on + strand, within AMB_RS12405at 2648.608 kb on - strandat 2648.781 kb on + strandat 2648.805 kb on + strandat 2648.805 kb on + strandat 2648.806 kb on - strandat 2648.806 kb on - strandat 2648.836 kb on + strandat 2648.857 kb on + strandat 2648.857 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 30_nonmag_3
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2,644,909 + AMB_RS12390 0.82 -2.7
2,644,910 - AMB_RS12390 0.82 -1.2
2,644,910 - AMB_RS12390 0.82 -1.0
2,644,998 + AMB_RS12390 0.85 +0.3
2,645,028 + AMB_RS12390 0.86 -0.9
2,645,064 + AMB_RS12390 0.87 -1.1
2,645,286 + -0.3
2,645,287 - +0.5
2,645,394 - -2.0
2,645,785 - AMB_RS12395 0.85 -1.1
2,645,785 - AMB_RS12395 0.85 -0.0
2,645,785 - AMB_RS12395 0.85 -0.2
2,645,785 - AMB_RS12395 0.85 -1.3
2,645,815 + -0.4
2,645,816 - -0.1
2,645,821 - -0.1
2,645,822 + -2.9
2,645,823 - -0.6
2,645,823 - -1.1
2,645,823 - -0.4
2,647,921 + +1.0
2,647,974 + +0.4
2,647,974 + -0.2
2,647,975 - +0.1
2,647,975 - -0.4
2,647,975 - +0.3
2,648,100 + AMB_RS12405 0.13 +0.1
2,648,100 + AMB_RS12405 0.13 +0.9
2,648,608 - +0.7
2,648,781 + +0.5
2,648,805 + -0.7
2,648,805 + +0.1
2,648,806 - -3.4
2,648,806 - +0.0
2,648,836 + -0.3
2,648,857 + -0.9
2,648,857 + -0.1

Or see this region's nucleotide sequence