Experiment: 30_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS20640 and AMB_RS20645 overlap by 4 nucleotides AMB_RS20645 and AMB_RS23800 are separated by 71 nucleotides AMB_RS23800 and AMB_RS20655 overlap by 1 nucleotides AMB_RS20655 and AMB_RS20660 are separated by 89 nucleotides
AMB_RS20640: AMB_RS20640 - glutamate-5-semialdehyde dehydrogenase, at 4,484,500 to 4,485,768
_RS20640
AMB_RS20645: AMB_RS20645 - nicotinate-nucleotide adenylyltransferase, at 4,485,765 to 4,486,376
_RS20645
AMB_RS23800: AMB_RS23800 - ribosome silencing factor, at 4,486,448 to 4,486,804
_RS23800
AMB_RS20655: AMB_RS20655 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 4,486,804 to 4,487,262
_RS20655
AMB_RS20660: AMB_RS20660 - hypothetical protein, at 4,487,352 to 4,487,867
_RS20660
Position (kb)
4485
4486
4487 Strain fitness (log2 ratio)
-1
0
1 at 4485.755 kb on + strand at 4486.375 kb on - strand at 4486.375 kb on - strand at 4486.375 kb on - strand at 4486.712 kb on + strand, within AMB_RS23800 at 4486.712 kb on + strand, within AMB_RS23800 at 4486.712 kb on + strand, within AMB_RS23800 at 4486.712 kb on + strand, within AMB_RS23800 at 4486.713 kb on - strand, within AMB_RS23800 at 4486.713 kb on - strand, within AMB_RS23800 at 4486.870 kb on + strand, within AMB_RS20655 at 4486.870 kb on + strand, within AMB_RS20655 at 4486.871 kb on - strand, within AMB_RS20655 at 4486.871 kb on - strand, within AMB_RS20655 at 4487.249 kb on - strand at 4487.369 kb on + strand at 4487.370 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_nonmag_2 remove 4,485,755 + +0.6 4,486,375 - -1.1 4,486,375 - +0.3 4,486,375 - -1.2 4,486,712 + AMB_RS23800 0.74 -0.2 4,486,712 + AMB_RS23800 0.74 -1.0 4,486,712 + AMB_RS23800 0.74 -1.0 4,486,712 + AMB_RS23800 0.74 -0.3 4,486,713 - AMB_RS23800 0.74 -1.5 4,486,713 - AMB_RS23800 0.74 -0.2 4,486,870 + AMB_RS20655 0.14 -1.0 4,486,870 + AMB_RS20655 0.14 +1.6 4,486,871 - AMB_RS20655 0.15 +0.2 4,486,871 - AMB_RS20655 0.15 +1.6 4,487,249 - +0.5 4,487,369 + +0.1 4,487,370 - +0.6
Or see this region's nucleotide sequence