Experiment: 30_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS18865 and AMB_RS18870 are separated by 10 nucleotides AMB_RS18870 and AMB_RS18875 overlap by 4 nucleotides AMB_RS18875 and AMB_RS18880 are separated by 70 nucleotides AMB_RS18880 and AMB_RS18885 are separated by 344 nucleotides
AMB_RS18865: AMB_RS18865 - homoserine dehydrogenase, at 4,101,511 to 4,102,797
_RS18865
AMB_RS18870: AMB_RS18870 - fructose-bisphosphatase class II, at 4,102,808 to 4,103,800
_RS18870
AMB_RS18875: AMB_RS18875 - single-stranded-DNA-specific exonuclease RecJ, at 4,103,797 to 4,105,590
_RS18875
AMB_RS18880: AMB_RS18880 - tRNA-Glu, at 4,105,661 to 4,105,736
_RS18880
AMB_RS18885: AMB_RS18885 - hypothetical protein, at 4,106,081 to 4,106,428
_RS18885
Position (kb)
4103
4104
4105
4106 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4103.192 kb on + strand, within AMB_RS18870 at 4103.192 kb on + strand, within AMB_RS18870 at 4103.192 kb on + strand, within AMB_RS18870 at 4103.192 kb on + strand, within AMB_RS18870 at 4103.192 kb on + strand, within AMB_RS18870 at 4103.192 kb on + strand, within AMB_RS18870 at 4103.192 kb on + strand, within AMB_RS18870 at 4103.363 kb on + strand, within AMB_RS18870 at 4103.363 kb on + strand, within AMB_RS18870 at 4103.363 kb on + strand, within AMB_RS18870 at 4103.363 kb on + strand, within AMB_RS18870 at 4103.453 kb on + strand, within AMB_RS18870 at 4103.453 kb on + strand, within AMB_RS18870 at 4103.453 kb on + strand, within AMB_RS18870 at 4103.453 kb on + strand, within AMB_RS18870 at 4103.453 kb on + strand, within AMB_RS18870 at 4103.714 kb on + strand at 4105.591 kb on + strand at 4105.592 kb on - strand at 4105.607 kb on + strand at 4105.607 kb on + strand at 4105.607 kb on + strand at 4105.848 kb on + strand at 4105.848 kb on + strand at 4105.849 kb on - strand at 4105.849 kb on - strand at 4105.849 kb on - strand at 4105.849 kb on - strand at 4105.849 kb on - strand at 4105.849 kb on - strand at 4105.849 kb on - strand at 4105.852 kb on + strand at 4105.852 kb on + strand at 4105.852 kb on + strand at 4105.852 kb on + strand at 4105.853 kb on - strand at 4105.853 kb on - strand at 4105.853 kb on - strand at 4105.853 kb on - strand at 4105.853 kb on - strand at 4105.859 kb on + strand at 4105.859 kb on + strand at 4105.859 kb on + strand at 4105.860 kb on - strand at 4105.915 kb on + strand at 4105.915 kb on + strand at 4105.916 kb on - strand at 4105.963 kb on + strand at 4105.963 kb on + strand at 4105.964 kb on - strand at 4105.964 kb on - strand at 4105.964 kb on - strand at 4106.013 kb on - strand at 4106.082 kb on + strand at 4106.109 kb on - strand at 4106.234 kb on + strand, within AMB_RS18885 at 4106.234 kb on + strand, within AMB_RS18885 at 4106.234 kb on + strand, within AMB_RS18885 at 4106.234 kb on + strand, within AMB_RS18885 at 4106.235 kb on - strand, within AMB_RS18885 at 4106.235 kb on - strand, within AMB_RS18885 at 4106.242 kb on + strand, within AMB_RS18885 at 4106.382 kb on + strand, within AMB_RS18885 at 4106.382 kb on + strand, within AMB_RS18885 at 4106.382 kb on + strand, within AMB_RS18885 at 4106.382 kb on + strand, within AMB_RS18885 at 4106.383 kb on - strand, within AMB_RS18885 at 4106.383 kb on - strand, within AMB_RS18885 at 4106.430 kb on + strand at 4106.431 kb on - strand at 4106.442 kb on - strand at 4106.442 kb on - strand at 4106.463 kb on + strand at 4106.463 kb on + strand at 4106.464 kb on - strand at 4106.464 kb on - strand at 4106.464 kb on - strand at 4106.464 kb on - strand at 4106.464 kb on - strand at 4106.468 kb on + strand at 4106.468 kb on + strand at 4106.468 kb on + strand at 4106.468 kb on + strand at 4106.468 kb on + strand at 4106.469 kb on - strand at 4106.469 kb on - strand at 4106.469 kb on - strand at 4106.469 kb on - strand at 4106.504 kb on + strand at 4106.504 kb on + strand at 4106.504 kb on + strand at 4106.504 kb on + strand at 4106.505 kb on - strand at 4106.505 kb on - strand at 4106.540 kb on + strand at 4106.541 kb on - strand at 4106.541 kb on - strand at 4106.541 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_nonmag_2 remove 4,103,192 + AMB_RS18870 0.39 -1.3 4,103,192 + AMB_RS18870 0.39 -0.1 4,103,192 + AMB_RS18870 0.39 +0.3 4,103,192 + AMB_RS18870 0.39 +0.6 4,103,192 + AMB_RS18870 0.39 -1.3 4,103,192 + AMB_RS18870 0.39 -0.7 4,103,192 + AMB_RS18870 0.39 -1.1 4,103,363 + AMB_RS18870 0.56 +0.9 4,103,363 + AMB_RS18870 0.56 -0.6 4,103,363 + AMB_RS18870 0.56 -0.9 4,103,363 + AMB_RS18870 0.56 +0.0 4,103,453 + AMB_RS18870 0.65 -3.9 4,103,453 + AMB_RS18870 0.65 +0.1 4,103,453 + AMB_RS18870 0.65 +0.1 4,103,453 + AMB_RS18870 0.65 -1.3 4,103,453 + AMB_RS18870 0.65 -2.7 4,103,714 + -0.5 4,105,591 + -3.5 4,105,592 - -1.3 4,105,607 + -2.2 4,105,607 + -1.0 4,105,607 + -3.3 4,105,848 + +0.4 4,105,848 + -2.0 4,105,849 - -0.0 4,105,849 - +1.6 4,105,849 - +1.0 4,105,849 - +1.1 4,105,849 - +1.6 4,105,849 - +0.1 4,105,849 - -1.1 4,105,852 + -3.7 4,105,852 + -0.8 4,105,852 + -1.0 4,105,852 + -1.3 4,105,853 - -2.9 4,105,853 - +0.4 4,105,853 - +0.8 4,105,853 - -0.1 4,105,853 - +0.1 4,105,859 + +0.6 4,105,859 + +0.6 4,105,859 + +0.2 4,105,860 - +0.7 4,105,915 + +0.3 4,105,915 + +1.1 4,105,916 - -0.1 4,105,963 + -0.5 4,105,963 + +3.0 4,105,964 - +0.4 4,105,964 - -1.5 4,105,964 - +0.0 4,106,013 - +0.0 4,106,082 + -1.0 4,106,109 - +0.9 4,106,234 + AMB_RS18885 0.44 -0.4 4,106,234 + AMB_RS18885 0.44 -0.2 4,106,234 + AMB_RS18885 0.44 +0.4 4,106,234 + AMB_RS18885 0.44 -0.6 4,106,235 - AMB_RS18885 0.44 +0.3 4,106,235 - AMB_RS18885 0.44 -0.2 4,106,242 + AMB_RS18885 0.46 +0.2 4,106,382 + AMB_RS18885 0.86 +0.4 4,106,382 + AMB_RS18885 0.86 -2.0 4,106,382 + AMB_RS18885 0.86 -0.7 4,106,382 + AMB_RS18885 0.86 -0.3 4,106,383 - AMB_RS18885 0.87 -1.2 4,106,383 - AMB_RS18885 0.87 -0.5 4,106,430 + +0.2 4,106,431 - -0.7 4,106,442 - -0.5 4,106,442 - -0.6 4,106,463 + +0.6 4,106,463 + +0.0 4,106,464 - -1.0 4,106,464 - -0.4 4,106,464 - +1.2 4,106,464 - -0.5 4,106,464 - -0.1 4,106,468 + +0.0 4,106,468 + +1.7 4,106,468 + -0.4 4,106,468 + +1.0 4,106,468 + -0.0 4,106,469 - -0.6 4,106,469 - -1.2 4,106,469 - -0.9 4,106,469 - -0.2 4,106,504 + -0.4 4,106,504 + -0.4 4,106,504 + -0.6 4,106,504 + +0.0 4,106,505 - -0.3 4,106,505 - -0.2 4,106,540 + -0.3 4,106,541 - -1.6 4,106,541 - +0.4 4,106,541 - -0.9
Or see this region's nucleotide sequence