Experiment: 30_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS14795 and AMB_RS14800 are separated by 39 nucleotides AMB_RS14800 and AMB_RS14805 are separated by 5 nucleotides AMB_RS14805 and AMB_RS14810 overlap by 4 nucleotides AMB_RS14810 and AMB_RS14815 overlap by 4 nucleotides AMB_RS14815 and AMB_RS14820 are separated by 57 nucleotides
AMB_RS14795: AMB_RS14795 - hypothetical protein, at 3,143,782 to 3,144,123
_RS14795
AMB_RS14800: AMB_RS14800 - hypothetical protein, at 3,144,163 to 3,144,426
_RS14800
AMB_RS14805: AMB_RS14805 - hypothetical protein, at 3,144,432 to 3,144,992
_RS14805
AMB_RS14810: AMB_RS14810 - VWA domain-containing protein, at 3,144,989 to 3,146,881
_RS14810
AMB_RS14815: AMB_RS14815 - CbbQ/NirQ/NorQ/GpvN family protein, at 3,146,878 to 3,147,660
_RS14815
AMB_RS14820: AMB_RS14820 - nitric oxide reductase, at 3,147,718 to 3,149,037
_RS14820
Position (kb)
3144
3145
3146
3147 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3144.040 kb on + strand, within AMB_RS14795 at 3144.040 kb on + strand, within AMB_RS14795 at 3144.040 kb on + strand, within AMB_RS14795 at 3144.041 kb on - strand, within AMB_RS14795 at 3144.041 kb on - strand, within AMB_RS14795 at 3144.273 kb on - strand, within AMB_RS14800 at 3144.445 kb on + strand at 3144.445 kb on + strand at 3144.445 kb on + strand at 3144.445 kb on + strand at 3144.446 kb on - strand at 3144.599 kb on - strand, within AMB_RS14805 at 3144.599 kb on - strand, within AMB_RS14805 at 3144.599 kb on - strand, within AMB_RS14805 at 3144.618 kb on - strand, within AMB_RS14805 at 3145.059 kb on + strand at 3145.060 kb on - strand at 3145.060 kb on - strand at 3145.090 kb on - strand at 3145.154 kb on + strand at 3145.194 kb on + strand, within AMB_RS14810 at 3145.194 kb on + strand, within AMB_RS14810 at 3145.194 kb on + strand, within AMB_RS14810 at 3145.194 kb on + strand, within AMB_RS14810 at 3145.195 kb on - strand, within AMB_RS14810 at 3145.195 kb on - strand, within AMB_RS14810 at 3145.230 kb on - strand, within AMB_RS14810 at 3145.308 kb on + strand, within AMB_RS14810 at 3145.308 kb on + strand, within AMB_RS14810 at 3145.309 kb on - strand, within AMB_RS14810 at 3145.309 kb on - strand, within AMB_RS14810 at 3145.825 kb on - strand, within AMB_RS14810 at 3145.825 kb on - strand, within AMB_RS14810 at 3146.253 kb on + strand, within AMB_RS14810 at 3146.254 kb on - strand, within AMB_RS14810 at 3146.330 kb on + strand, within AMB_RS14810 at 3146.331 kb on - strand, within AMB_RS14810 at 3146.545 kb on - strand, within AMB_RS14810 at 3146.545 kb on - strand, within AMB_RS14810 at 3146.896 kb on + strand at 3146.896 kb on + strand at 3147.008 kb on + strand, within AMB_RS14815 at 3147.008 kb on + strand, within AMB_RS14815 at 3147.009 kb on - strand, within AMB_RS14815 at 3147.009 kb on - strand, within AMB_RS14815 at 3147.236 kb on + strand, within AMB_RS14815 at 3147.236 kb on + strand, within AMB_RS14815 at 3147.236 kb on + strand, within AMB_RS14815 at 3147.236 kb on + strand, within AMB_RS14815 at 3147.237 kb on - strand, within AMB_RS14815 at 3147.237 kb on - strand, within AMB_RS14815 at 3147.450 kb on - strand, within AMB_RS14815 at 3147.639 kb on - strand at 3147.719 kb on + strand at 3147.719 kb on + strand at 3147.720 kb on - strand at 3147.720 kb on - strand at 3147.815 kb on + strand at 3147.815 kb on + strand at 3147.815 kb on + strand at 3147.816 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_nonmag_2 remove 3,144,040 + AMB_RS14795 0.75 +1.2 3,144,040 + AMB_RS14795 0.75 +0.7 3,144,040 + AMB_RS14795 0.75 -0.8 3,144,041 - AMB_RS14795 0.76 -0.1 3,144,041 - AMB_RS14795 0.76 +0.5 3,144,273 - AMB_RS14800 0.42 -1.5 3,144,445 + -0.6 3,144,445 + -0.2 3,144,445 + -0.3 3,144,445 + -0.4 3,144,446 - -1.5 3,144,599 - AMB_RS14805 0.30 -1.2 3,144,599 - AMB_RS14805 0.30 +0.1 3,144,599 - AMB_RS14805 0.30 +0.7 3,144,618 - AMB_RS14805 0.33 -0.3 3,145,059 + +0.5 3,145,060 - +1.3 3,145,060 - -2.3 3,145,090 - -1.8 3,145,154 + -0.2 3,145,194 + AMB_RS14810 0.11 +0.9 3,145,194 + AMB_RS14810 0.11 +0.4 3,145,194 + AMB_RS14810 0.11 +0.5 3,145,194 + AMB_RS14810 0.11 -0.0 3,145,195 - AMB_RS14810 0.11 +0.3 3,145,195 - AMB_RS14810 0.11 -0.8 3,145,230 - AMB_RS14810 0.13 +0.3 3,145,308 + AMB_RS14810 0.17 -2.6 3,145,308 + AMB_RS14810 0.17 -0.1 3,145,309 - AMB_RS14810 0.17 +0.3 3,145,309 - AMB_RS14810 0.17 +0.6 3,145,825 - AMB_RS14810 0.44 +0.9 3,145,825 - AMB_RS14810 0.44 +0.1 3,146,253 + AMB_RS14810 0.67 +0.5 3,146,254 - AMB_RS14810 0.67 +0.4 3,146,330 + AMB_RS14810 0.71 +0.2 3,146,331 - AMB_RS14810 0.71 +0.4 3,146,545 - AMB_RS14810 0.82 +0.7 3,146,545 - AMB_RS14810 0.82 +0.4 3,146,896 + -2.8 3,146,896 + +1.6 3,147,008 + AMB_RS14815 0.17 -0.7 3,147,008 + AMB_RS14815 0.17 +2.0 3,147,009 - AMB_RS14815 0.17 +1.5 3,147,009 - AMB_RS14815 0.17 +0.4 3,147,236 + AMB_RS14815 0.46 +1.4 3,147,236 + AMB_RS14815 0.46 +0.5 3,147,236 + AMB_RS14815 0.46 +1.5 3,147,236 + AMB_RS14815 0.46 -0.5 3,147,237 - AMB_RS14815 0.46 +1.5 3,147,237 - AMB_RS14815 0.46 +0.5 3,147,450 - AMB_RS14815 0.73 +0.7 3,147,639 - +2.4 3,147,719 + +0.8 3,147,719 + +0.4 3,147,720 - -2.0 3,147,720 - +0.8 3,147,815 + +1.5 3,147,815 + +0.8 3,147,815 + -0.5 3,147,816 - -0.2
Or see this region's nucleotide sequence