Experiment: 30_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS13885 and AMB_RS13890 overlap by 4 nucleotides AMB_RS13890 and AMB_RS13895 overlap by 4 nucleotides AMB_RS13895 and AMB_RS13900 are separated by 26 nucleotides
AMB_RS13885: AMB_RS13885 - methyltransferase domain-containing protein, at 2,946,892 to 2,947,626
_RS13885
AMB_RS13890: AMB_RS13890 - ATP-dependent dethiobiotin synthetase BioD, at 2,947,623 to 2,948,240
_RS13890
AMB_RS13895: AMB_RS13895 - adenosylmethionine--8-amino-7-oxononanoate transaminase, at 2,948,237 to 2,949,556
_RS13895
AMB_RS13900: AMB_RS13900 - efflux RND transporter periplasmic adaptor subunit, at 2,949,583 to 2,950,590
_RS13900
Position (kb)
2948
2949
2950 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2947.559 kb on + strand at 2948.219 kb on + strand at 2948.219 kb on + strand at 2949.554 kb on + strand at 2949.555 kb on - strand at 2949.568 kb on + strand at 2949.568 kb on + strand at 2949.568 kb on + strand at 2949.569 kb on - strand at 2949.586 kb on + strand at 2949.586 kb on + strand at 2949.587 kb on - strand at 2949.587 kb on - strand at 2949.587 kb on - strand at 2949.693 kb on - strand, within AMB_RS13900 at 2949.693 kb on - strand, within AMB_RS13900 at 2949.727 kb on + strand, within AMB_RS13900 at 2949.727 kb on + strand, within AMB_RS13900 at 2949.728 kb on - strand, within AMB_RS13900 at 2949.905 kb on - strand, within AMB_RS13900 at 2949.991 kb on + strand, within AMB_RS13900 at 2949.991 kb on + strand, within AMB_RS13900 at 2950.155 kb on + strand, within AMB_RS13900 at 2950.156 kb on - strand, within AMB_RS13900
Per-strain Table
Position Strand Gene LocusTag Fraction 30_nonmag_2 remove 2,947,559 + +0.5 2,948,219 + -0.6 2,948,219 + +1.5 2,949,554 + -1.8 2,949,555 - +0.1 2,949,568 + -0.0 2,949,568 + +1.5 2,949,568 + -0.2 2,949,569 - -0.4 2,949,586 + -1.8 2,949,586 + +0.2 2,949,587 - -0.6 2,949,587 - +1.3 2,949,587 - +0.5 2,949,693 - AMB_RS13900 0.11 -2.8 2,949,693 - AMB_RS13900 0.11 -2.2 2,949,727 + AMB_RS13900 0.14 +1.5 2,949,727 + AMB_RS13900 0.14 -0.6 2,949,728 - AMB_RS13900 0.14 -2.8 2,949,905 - AMB_RS13900 0.32 -0.2 2,949,991 + AMB_RS13900 0.40 +0.4 2,949,991 + AMB_RS13900 0.40 -1.3 2,950,155 + AMB_RS13900 0.57 -0.3 2,950,156 - AMB_RS13900 0.57 +0.2
Or see this region's nucleotide sequence