Experiment: 30_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03910 and AMB_RS03915 are separated by 221 nucleotides AMB_RS03915 and AMB_RS03920 are separated by 116 nucleotides
AMB_RS03910: AMB_RS03910 - homoserine kinase, at 819,536 to 820,516
_RS03910
AMB_RS03915: AMB_RS03915 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 820,738 to 821,694
_RS03915
AMB_RS03920: AMB_RS03920 - hypothetical protein, at 821,811 to 822,356
_RS03920
Position (kb) 820
821
822 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 820.560 kb on - strand at 820.560 kb on - strand at 820.560 kb on - strand at 820.678 kb on + strand at 820.679 kb on - strand at 820.739 kb on + strand at 820.739 kb on + strand at 820.739 kb on + strand at 820.740 kb on - strand at 821.761 kb on - strand at 821.798 kb on - strand at 821.931 kb on + strand, within AMB_RS03920 at 821.931 kb on + strand, within AMB_RS03920 at 821.932 kb on - strand, within AMB_RS03920 at 821.965 kb on - strand, within AMB_RS03920 at 821.965 kb on - strand, within AMB_RS03920 at 822.042 kb on + strand, within AMB_RS03920 at 822.043 kb on - strand, within AMB_RS03920 at 822.043 kb on - strand, within AMB_RS03920 at 822.133 kb on - strand, within AMB_RS03920 at 822.354 kb on + strand at 822.394 kb on - strand at 822.394 kb on - strand at 822.539 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_nonmag_2 remove 820,560 - +1.3 820,560 - -0.8 820,560 - +0.9 820,678 + -1.7 820,679 - +2.0 820,739 + -1.4 820,739 + -0.9 820,739 + +1.1 820,740 - +0.6 821,761 - -2.3 821,798 - -2.0 821,931 + AMB_RS03920 0.22 -2.0 821,931 + AMB_RS03920 0.22 +0.6 821,932 - AMB_RS03920 0.22 +0.5 821,965 - AMB_RS03920 0.28 +0.3 821,965 - AMB_RS03920 0.28 -1.7 822,042 + AMB_RS03920 0.42 -1.0 822,043 - AMB_RS03920 0.42 -2.6 822,043 - AMB_RS03920 0.42 -1.5 822,133 - AMB_RS03920 0.59 -3.0 822,354 + +0.1 822,394 - +0.4 822,394 - +0.7 822,539 + +0.3
Or see this region's nucleotide sequence