Experiment: 30_nonmag_2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AMB_RS03565 and AMB_RS03570 are separated by 237 nucleotides AMB_RS03570 and AMB_RS03575 are separated by 79 nucleotides AMB_RS03575 and AMB_RS03580 are separated by 77 nucleotides AMB_RS03580 and AMB_RS03585 are separated by 188 nucleotides
AMB_RS03565: AMB_RS03565 - pyridoxal phosphate-dependent aminotransferase, at 747,699 to 748,859
_RS03565
AMB_RS03570: AMB_RS03570 - P-II family nitrogen regulator, at 749,097 to 749,435
_RS03570
AMB_RS03575: AMB_RS03575 - type I glutamate--ammonia ligase, at 749,515 to 750,927
_RS03575
AMB_RS03580: AMB_RS03580 - tRNA-Met, at 751,005 to 751,080
_RS03580
AMB_RS03585: AMB_RS03585 - RNA polymerase sigma factor RpoD, at 751,269 to 753,308
_RS03585
Position (kb) 749
750
751 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 748.621 kb on + strand, within AMB_RS03565 at 748.622 kb on - strand, within AMB_RS03565 at 748.622 kb on - strand, within AMB_RS03565 at 748.729 kb on - strand, within AMB_RS03565 at 748.729 kb on - strand, within AMB_RS03565 at 748.897 kb on + strand at 748.953 kb on - strand at 748.967 kb on - strand at 749.247 kb on + strand, within AMB_RS03570 at 749.267 kb on + strand, within AMB_RS03570 at 749.267 kb on + strand, within AMB_RS03570 at 751.110 kb on - strand at 751.265 kb on + strand at 751.265 kb on + strand at 751.265 kb on + strand at 751.265 kb on + strand at 751.266 kb on - strand at 751.266 kb on - strand at 751.275 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 30_nonmag_2 remove 748,621 + AMB_RS03565 0.79 -1.7 748,622 - AMB_RS03565 0.80 -0.4 748,622 - AMB_RS03565 0.80 -0.0 748,729 - AMB_RS03565 0.89 -2.4 748,729 - AMB_RS03565 0.89 +0.1 748,897 + +0.5 748,953 - -3.9 748,967 - -1.1 749,247 + AMB_RS03570 0.44 -1.7 749,267 + AMB_RS03570 0.50 -1.0 749,267 + AMB_RS03570 0.50 +0.6 751,110 - -1.0 751,265 + +0.2 751,265 + +1.2 751,265 + +0.3 751,265 + +0.1 751,266 - -0.9 751,266 - -0.4 751,275 - +0.4
Or see this region's nucleotide sequence