Experiment: M63_quarter_strength
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt UW163_RS02650 and UW163_RS02655 are separated by 99 nucleotides UW163_RS02655 and UW163_RS02660 are separated by 19 nucleotides UW163_RS02660 and UW163_RS02665 are separated by 87 nucleotides UW163_RS02665 and UW163_RS02670 are separated by 151 nucleotides
UW163_RS02650: UW163_RS02650 - amidophosphoribosyltransferase, at 553,187 to 553,909
_RS02650
UW163_RS02655: UW163_RS02655 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 554,009 to 554,479
_RS02655
UW163_RS02660: UW163_RS02660 - glycerol-3-phosphate dehydrogenase, at 554,499 to 555,515
_RS02660
UW163_RS02665: UW163_RS02665 - protein-export chaperone SecB, at 555,603 to 556,124
_RS02665
UW163_RS02670: UW163_RS02670 - glutaredoxin 3, at 556,276 to 556,533
_RS02670
Position (kb) 554
555
556 Strain fitness (log2 ratio) -1
0
1
2 at 553.833 kb on + strand, within UW163_RS02650 at 554.160 kb on - strand, within UW163_RS02655 at 554.436 kb on + strand at 555.157 kb on + strand at 555.601 kb on - strand at 555.698 kb on - strand, within UW163_RS02665 at 556.151 kb on - strand at 556.190 kb on - strand at 556.194 kb on + strand at 556.195 kb on - strand at 556.361 kb on + strand, within UW163_RS02670 at 556.362 kb on - strand, within UW163_RS02670 at 556.362 kb on - strand, within UW163_RS02670 at 556.447 kb on - strand, within UW163_RS02670 at 556.514 kb on + strand at 556.515 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M63_quarter_strength remove 553,833 + UW163_RS02650 0.89 -1.0 554,160 - UW163_RS02655 0.32 -0.4 554,436 + -0.1 555,157 + +2.4 555,601 - +0.9 555,698 - UW163_RS02665 0.18 +0.6 556,151 - -0.3 556,190 - +0.3 556,194 + -0.5 556,195 - -0.6 556,361 + UW163_RS02670 0.33 -0.1 556,362 - UW163_RS02670 0.33 -0.6 556,362 - UW163_RS02670 0.33 -0.1 556,447 - UW163_RS02670 0.66 -0.4 556,514 + -0.2 556,515 - -1.2
Or see this region's nucleotide sequence