Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4491 and EX28DRAFT_4492 are separated by 82 nucleotides EX28DRAFT_4492 and EX28DRAFT_4493 overlap by 113 nucleotides EX28DRAFT_4493 and EX28DRAFT_4494 are separated by 115 nucleotides
EX28DRAFT_4491: EX28DRAFT_4491 - HAD-superfamily hydrolase, subfamily IIB, at 57,035 to 57,835
_4491
EX28DRAFT_4492: EX28DRAFT_4492 - Carboxylate/Amino Acid/Amine Transporter, at 57,918 to 58,817
_4492
EX28DRAFT_4493: EX28DRAFT_4493 - Transcriptional regulator, at 58,705 to 59,658
_4493
EX28DRAFT_4494: EX28DRAFT_4494 - methionine synthase (B12-independent) (EC 2.1.1.14), at 59,774 to 62,035
_4494
Position (kb) 58
59
60 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 57.784 kb on + strand at 57.809 kb on + strand at 57.809 kb on + strand at 57.809 kb on + strand at 57.817 kb on + strand at 57.834 kb on - strand at 57.834 kb on - strand at 57.882 kb on + strand at 57.925 kb on + strand at 57.941 kb on + strand at 58.095 kb on + strand, within EX28DRAFT_4492 at 58.096 kb on + strand, within EX28DRAFT_4492 at 58.096 kb on - strand, within EX28DRAFT_4492 at 58.097 kb on + strand, within EX28DRAFT_4492 at 58.097 kb on + strand, within EX28DRAFT_4492 at 58.098 kb on - strand, within EX28DRAFT_4492 at 58.098 kb on - strand, within EX28DRAFT_4492 at 58.098 kb on - strand, within EX28DRAFT_4492 at 58.131 kb on + strand, within EX28DRAFT_4492 at 58.131 kb on + strand, within EX28DRAFT_4492 at 58.131 kb on + strand, within EX28DRAFT_4492 at 58.131 kb on + strand, within EX28DRAFT_4492 at 58.132 kb on - strand, within EX28DRAFT_4492 at 58.206 kb on + strand, within EX28DRAFT_4492 at 58.305 kb on + strand, within EX28DRAFT_4492 at 58.389 kb on + strand, within EX28DRAFT_4492 at 58.428 kb on - strand, within EX28DRAFT_4492 at 58.446 kb on + strand, within EX28DRAFT_4492 at 58.446 kb on + strand, within EX28DRAFT_4492 at 58.446 kb on + strand, within EX28DRAFT_4492 at 58.447 kb on - strand, within EX28DRAFT_4492 at 58.447 kb on - strand, within EX28DRAFT_4492 at 58.703 kb on + strand, within EX28DRAFT_4492 at 58.704 kb on - strand, within EX28DRAFT_4492 at 58.865 kb on + strand, within EX28DRAFT_4493 at 58.866 kb on - strand, within EX28DRAFT_4493 at 58.866 kb on - strand, within EX28DRAFT_4493 at 59.177 kb on + strand, within EX28DRAFT_4493 at 59.358 kb on + strand, within EX28DRAFT_4493 at 59.387 kb on - strand, within EX28DRAFT_4493 at 59.387 kb on - strand, within EX28DRAFT_4493 at 59.387 kb on - strand, within EX28DRAFT_4493 at 59.506 kb on - strand, within EX28DRAFT_4493 at 59.685 kb on + strand at 59.701 kb on + strand at 59.701 kb on + strand at 59.702 kb on - strand at 59.702 kb on - strand at 59.702 kb on - strand at 59.702 kb on - strand at 59.703 kb on + strand at 59.703 kb on + strand at 59.704 kb on - strand at 59.704 kb on - strand at 59.708 kb on + strand at 59.722 kb on - strand at 59.722 kb on - strand at 59.770 kb on + strand at 59.770 kb on + strand at 59.770 kb on + strand at 59.771 kb on - strand at 59.850 kb on - strand at 60.045 kb on + strand, within EX28DRAFT_4494 at 60.045 kb on + strand, within EX28DRAFT_4494 at 60.046 kb on - strand, within EX28DRAFT_4494 at 60.049 kb on + strand, within EX28DRAFT_4494 at 60.050 kb on - strand, within EX28DRAFT_4494 at 60.140 kb on + strand, within EX28DRAFT_4494 at 60.140 kb on + strand, within EX28DRAFT_4494 at 60.141 kb on - strand, within EX28DRAFT_4494 at 60.145 kb on + strand, within EX28DRAFT_4494 at 60.146 kb on + strand, within EX28DRAFT_4494 at 60.146 kb on + strand, within EX28DRAFT_4494 at 60.146 kb on + strand, within EX28DRAFT_4494 at 60.147 kb on - strand, within EX28DRAFT_4494 at 60.147 kb on - strand, within EX28DRAFT_4494 at 60.147 kb on - strand, within EX28DRAFT_4494 at 60.147 kb on - strand, within EX28DRAFT_4494 at 60.147 kb on - strand, within EX28DRAFT_4494 at 60.148 kb on + strand, within EX28DRAFT_4494 at 60.148 kb on + strand, within EX28DRAFT_4494 at 60.148 kb on + strand, within EX28DRAFT_4494 at 60.148 kb on + strand, within EX28DRAFT_4494 at 60.149 kb on - strand, within EX28DRAFT_4494 at 60.149 kb on - strand, within EX28DRAFT_4494 at 60.149 kb on - strand, within EX28DRAFT_4494 at 60.273 kb on - strand, within EX28DRAFT_4494 at 60.438 kb on + strand, within EX28DRAFT_4494 at 60.439 kb on - strand, within EX28DRAFT_4494 at 60.500 kb on + strand, within EX28DRAFT_4494 at 60.501 kb on - strand, within EX28DRAFT_4494 at 60.501 kb on - strand, within EX28DRAFT_4494 at 60.574 kb on - strand, within EX28DRAFT_4494 at 60.580 kb on + strand, within EX28DRAFT_4494
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 57,784 + +1.8 57,809 + +1.0 57,809 + +0.6 57,809 + +0.2 57,817 + +0.1 57,834 - -0.5 57,834 - +1.7 57,882 + +0.1 57,925 + +0.8 57,941 + -1.1 58,095 + EX28DRAFT_4492 0.20 +0.5 58,096 + EX28DRAFT_4492 0.20 +1.5 58,096 - EX28DRAFT_4492 0.20 -0.3 58,097 + EX28DRAFT_4492 0.20 +0.6 58,097 + EX28DRAFT_4492 0.20 +1.0 58,098 - EX28DRAFT_4492 0.20 -0.6 58,098 - EX28DRAFT_4492 0.20 -4.4 58,098 - EX28DRAFT_4492 0.20 -0.6 58,131 + EX28DRAFT_4492 0.24 +0.0 58,131 + EX28DRAFT_4492 0.24 +1.9 58,131 + EX28DRAFT_4492 0.24 +0.7 58,131 + EX28DRAFT_4492 0.24 -0.3 58,132 - EX28DRAFT_4492 0.24 +0.6 58,206 + EX28DRAFT_4492 0.32 -0.5 58,305 + EX28DRAFT_4492 0.43 +0.2 58,389 + EX28DRAFT_4492 0.52 +0.6 58,428 - EX28DRAFT_4492 0.57 +0.5 58,446 + EX28DRAFT_4492 0.59 +0.7 58,446 + EX28DRAFT_4492 0.59 +0.7 58,446 + EX28DRAFT_4492 0.59 +0.5 58,447 - EX28DRAFT_4492 0.59 -0.9 58,447 - EX28DRAFT_4492 0.59 +0.8 58,703 + EX28DRAFT_4492 0.87 +1.1 58,704 - EX28DRAFT_4492 0.87 -1.5 58,865 + EX28DRAFT_4493 0.17 -5.0 58,866 - EX28DRAFT_4493 0.17 -3.2 58,866 - EX28DRAFT_4493 0.17 -2.3 59,177 + EX28DRAFT_4493 0.49 -6.8 59,358 + EX28DRAFT_4493 0.68 -3.2 59,387 - EX28DRAFT_4493 0.71 -5.1 59,387 - EX28DRAFT_4493 0.71 -4.6 59,387 - EX28DRAFT_4493 0.71 -4.3 59,506 - EX28DRAFT_4493 0.84 +0.4 59,685 + -5.6 59,701 + -5.0 59,701 + -5.1 59,702 - +0.9 59,702 - -2.1 59,702 - +1.0 59,702 - -0.3 59,703 + -4.6 59,703 + -0.5 59,704 - +0.0 59,704 - -0.7 59,708 + -4.2 59,722 - -0.4 59,722 - +1.7 59,770 + +0.0 59,770 + -1.3 59,770 + -0.6 59,771 - -0.6 59,850 - -0.7 60,045 + EX28DRAFT_4494 0.12 -0.2 60,045 + EX28DRAFT_4494 0.12 -3.6 60,046 - EX28DRAFT_4494 0.12 -0.6 60,049 + EX28DRAFT_4494 0.12 -1.2 60,050 - EX28DRAFT_4494 0.12 +0.1 60,140 + EX28DRAFT_4494 0.16 -2.0 60,140 + EX28DRAFT_4494 0.16 -0.8 60,141 - EX28DRAFT_4494 0.16 -0.6 60,145 + EX28DRAFT_4494 0.16 -0.2 60,146 + EX28DRAFT_4494 0.16 -1.0 60,146 + EX28DRAFT_4494 0.16 -1.5 60,146 + EX28DRAFT_4494 0.16 -0.8 60,147 - EX28DRAFT_4494 0.16 -0.5 60,147 - EX28DRAFT_4494 0.16 -0.6 60,147 - EX28DRAFT_4494 0.16 -1.2 60,147 - EX28DRAFT_4494 0.16 -0.2 60,147 - EX28DRAFT_4494 0.16 +0.2 60,148 + EX28DRAFT_4494 0.17 -0.7 60,148 + EX28DRAFT_4494 0.17 +0.1 60,148 + EX28DRAFT_4494 0.17 -1.0 60,148 + EX28DRAFT_4494 0.17 -2.9 60,149 - EX28DRAFT_4494 0.17 -2.3 60,149 - EX28DRAFT_4494 0.17 -0.6 60,149 - EX28DRAFT_4494 0.17 +0.5 60,273 - EX28DRAFT_4494 0.22 +0.2 60,438 + EX28DRAFT_4494 0.29 +0.1 60,439 - EX28DRAFT_4494 0.29 -2.3 60,500 + EX28DRAFT_4494 0.32 -0.8 60,501 - EX28DRAFT_4494 0.32 -1.9 60,501 - EX28DRAFT_4494 0.32 -1.5 60,574 - EX28DRAFT_4494 0.35 -2.1 60,580 + EX28DRAFT_4494 0.36 -2.0
Or see this region's nucleotide sequence