Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4475 and EX28DRAFT_4476 are separated by 95 nucleotides EX28DRAFT_4476 and EX28DRAFT_4477 are separated by 135 nucleotides EX28DRAFT_4477 and EX28DRAFT_4478 are separated by 35 nucleotides EX28DRAFT_4478 and EX28DRAFT_4479 overlap by 4 nucleotides EX28DRAFT_4479 and EX28DRAFT_4480 overlap by 4 nucleotides
EX28DRAFT_4475: EX28DRAFT_4475 - Adenylate cyclase, at 40,094 to 42,637
_4475
EX28DRAFT_4476: EX28DRAFT_4476 - iron donor protein CyaY, at 42,733 to 43,053
_4476
EX28DRAFT_4477: EX28DRAFT_4477 - Predicted small periplasmic lipoprotein, at 43,189 to 43,392
_4477
EX28DRAFT_4478: EX28DRAFT_4478 - diaminopimelate epimerase (EC 5.1.1.7), at 43,428 to 44,252
_4478
EX28DRAFT_4479: EX28DRAFT_4479 - Uncharacterized protein conserved in bacteria, at 44,249 to 44,956
_4479
EX28DRAFT_4480: EX28DRAFT_4480 - tyrosine recombinase XerC, at 44,953 to 45,855
_4480
Position (kb) 43
44
45 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 42.494 kb on - strand at 42.545 kb on - strand at 42.679 kb on + strand at 42.735 kb on - strand at 43.087 kb on - strand at 43.099 kb on - strand at 43.173 kb on + strand at 43.174 kb on - strand at 43.185 kb on + strand at 43.267 kb on + strand, within EX28DRAFT_4477 at 43.268 kb on - strand, within EX28DRAFT_4477 at 43.268 kb on - strand, within EX28DRAFT_4477 at 43.268 kb on - strand, within EX28DRAFT_4477 at 43.344 kb on + strand, within EX28DRAFT_4477 at 43.344 kb on + strand, within EX28DRAFT_4477 at 43.347 kb on + strand, within EX28DRAFT_4477 at 43.439 kb on + strand at 43.842 kb on + strand, within EX28DRAFT_4478 at 43.843 kb on - strand, within EX28DRAFT_4478 at 43.843 kb on - strand, within EX28DRAFT_4478 at 43.843 kb on - strand, within EX28DRAFT_4478 at 43.843 kb on - strand, within EX28DRAFT_4478 at 43.843 kb on - strand, within EX28DRAFT_4478 at 43.844 kb on + strand, within EX28DRAFT_4478 at 43.847 kb on + strand, within EX28DRAFT_4478 at 43.871 kb on - strand, within EX28DRAFT_4478 at 44.047 kb on - strand, within EX28DRAFT_4478 at 44.203 kb on - strand at 44.240 kb on + strand at 44.248 kb on + strand at 44.315 kb on + strand at 44.845 kb on - strand, within EX28DRAFT_4479 at 44.849 kb on - strand, within EX28DRAFT_4479
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 42,494 - -1.9 42,545 - -1.3 42,679 + -0.2 42,735 - +0.9 43,087 - +1.1 43,099 - -0.2 43,173 + +0.5 43,174 - +0.0 43,185 + +0.9 43,267 + EX28DRAFT_4477 0.38 -2.1 43,268 - EX28DRAFT_4477 0.39 -2.0 43,268 - EX28DRAFT_4477 0.39 -2.5 43,268 - EX28DRAFT_4477 0.39 +0.4 43,344 + EX28DRAFT_4477 0.76 +0.8 43,344 + EX28DRAFT_4477 0.76 +1.6 43,347 + EX28DRAFT_4477 0.77 -0.5 43,439 + -3.6 43,842 + EX28DRAFT_4478 0.50 -4.2 43,843 - EX28DRAFT_4478 0.50 -4.6 43,843 - EX28DRAFT_4478 0.50 -2.2 43,843 - EX28DRAFT_4478 0.50 -1.5 43,843 - EX28DRAFT_4478 0.50 -2.1 43,843 - EX28DRAFT_4478 0.50 -2.8 43,844 + EX28DRAFT_4478 0.50 -0.4 43,847 + EX28DRAFT_4478 0.51 -3.1 43,871 - EX28DRAFT_4478 0.54 -2.6 44,047 - EX28DRAFT_4478 0.75 -3.3 44,203 - -3.3 44,240 + +0.0 44,248 + +0.6 44,315 + +0.4 44,845 - EX28DRAFT_4479 0.84 +1.5 44,849 - EX28DRAFT_4479 0.85 -0.2
Or see this region's nucleotide sequence