Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4443 and EX28DRAFT_4444 overlap by 1 nucleotides EX28DRAFT_4444 and EX28DRAFT_4445 are separated by 45 nucleotides
EX28DRAFT_4443: EX28DRAFT_4443 - dihydroxy-acid dehydratase, at 6,825 to 8,675
_4443
EX28DRAFT_4444: EX28DRAFT_4444 - threonine ammonia-lyase, biosynthetic, long form, at 8,675 to 10,222
_4444
EX28DRAFT_4445: EX28DRAFT_4445 - Transcriptional regulator, at 10,268 to 11,158
_4445
Position (kb)
8
9
10
11 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 7.760 kb on + strand, within EX28DRAFT_4443 at 7.761 kb on - strand, within EX28DRAFT_4443 at 7.761 kb on - strand, within EX28DRAFT_4443 at 7.785 kb on + strand, within EX28DRAFT_4443 at 7.786 kb on - strand, within EX28DRAFT_4443 at 7.791 kb on - strand, within EX28DRAFT_4443 at 7.801 kb on + strand, within EX28DRAFT_4443 at 7.802 kb on - strand, within EX28DRAFT_4443 at 7.802 kb on - strand, within EX28DRAFT_4443 at 8.064 kb on + strand, within EX28DRAFT_4443 at 8.100 kb on + strand, within EX28DRAFT_4443 at 8.188 kb on - strand, within EX28DRAFT_4443 at 8.255 kb on + strand, within EX28DRAFT_4443 at 8.256 kb on - strand, within EX28DRAFT_4443 at 8.328 kb on - strand, within EX28DRAFT_4443 at 8.661 kb on - strand at 8.753 kb on + strand at 8.753 kb on + strand at 8.754 kb on - strand at 9.299 kb on - strand, within EX28DRAFT_4444 at 9.449 kb on + strand, within EX28DRAFT_4444 at 9.475 kb on + strand, within EX28DRAFT_4444 at 9.485 kb on - strand, within EX28DRAFT_4444 at 9.778 kb on + strand, within EX28DRAFT_4444 at 9.779 kb on - strand, within EX28DRAFT_4444 at 9.811 kb on + strand, within EX28DRAFT_4444 at 9.939 kb on - strand, within EX28DRAFT_4444 at 10.054 kb on + strand, within EX28DRAFT_4444 at 10.055 kb on - strand, within EX28DRAFT_4444 at 10.166 kb on + strand at 10.238 kb on + strand at 10.303 kb on + strand at 10.501 kb on - strand, within EX28DRAFT_4445 at 10.579 kb on + strand, within EX28DRAFT_4445 at 10.580 kb on - strand, within EX28DRAFT_4445 at 10.849 kb on + strand, within EX28DRAFT_4445 at 10.851 kb on + strand, within EX28DRAFT_4445 at 11.150 kb on + strand at 11.151 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 7,760 + EX28DRAFT_4443 0.51 -5.7 7,761 - EX28DRAFT_4443 0.51 -4.7 7,761 - EX28DRAFT_4443 0.51 -6.5 7,785 + EX28DRAFT_4443 0.52 -4.0 7,786 - EX28DRAFT_4443 0.52 -3.9 7,791 - EX28DRAFT_4443 0.52 -1.9 7,801 + EX28DRAFT_4443 0.53 -4.1 7,802 - EX28DRAFT_4443 0.53 -2.5 7,802 - EX28DRAFT_4443 0.53 -3.3 8,064 + EX28DRAFT_4443 0.67 -6.5 8,100 + EX28DRAFT_4443 0.69 -2.5 8,188 - EX28DRAFT_4443 0.74 -5.5 8,255 + EX28DRAFT_4443 0.77 -2.1 8,256 - EX28DRAFT_4443 0.77 -6.4 8,328 - EX28DRAFT_4443 0.81 -3.1 8,661 - -4.8 8,753 + -4.5 8,753 + -2.2 8,754 - -5.5 9,299 - EX28DRAFT_4444 0.40 -2.1 9,449 + EX28DRAFT_4444 0.50 -3.8 9,475 + EX28DRAFT_4444 0.52 -1.1 9,485 - EX28DRAFT_4444 0.52 -2.7 9,778 + EX28DRAFT_4444 0.71 -4.9 9,779 - EX28DRAFT_4444 0.71 -2.6 9,811 + EX28DRAFT_4444 0.73 -2.1 9,939 - EX28DRAFT_4444 0.82 -2.7 10,054 + EX28DRAFT_4444 0.89 -4.8 10,055 - EX28DRAFT_4444 0.89 -2.8 10,166 + -4.4 10,238 + +1.0 10,303 + -2.6 10,501 - EX28DRAFT_4445 0.26 -1.8 10,579 + EX28DRAFT_4445 0.35 -2.7 10,580 - EX28DRAFT_4445 0.35 -1.3 10,849 + EX28DRAFT_4445 0.65 -4.8 10,851 + EX28DRAFT_4445 0.65 -2.5 11,150 + -6.5 11,151 - -4.5
Or see this region's nucleotide sequence