Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4419 and EX28DRAFT_4420 are separated by 88 nucleotides EX28DRAFT_4420 and EX28DRAFT_4421 are separated by 9 nucleotides EX28DRAFT_4421 and EX28DRAFT_4422 are separated by 74 nucleotides
EX28DRAFT_4419: EX28DRAFT_4419 - acetylornithine deacetylase (ArgE), at 86,469 to 87,620
_4419
EX28DRAFT_4420: EX28DRAFT_4420 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 87,709 to 88,713
_4420
EX28DRAFT_4421: EX28DRAFT_4421 - N-acetylglutamate kinase (EC 2.7.2.8), at 88,723 to 89,496
_4421
EX28DRAFT_4422: EX28DRAFT_4422 - argininosuccinate lyase, at 89,571 to 90,944
_4422
Position (kb)
87
88
89 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 87.163 kb on + strand, within EX28DRAFT_4419 at 87.211 kb on + strand, within EX28DRAFT_4419 at 87.634 kb on + strand at 87.634 kb on + strand at 87.634 kb on + strand at 87.634 kb on + strand at 87.634 kb on + strand at 87.635 kb on - strand at 87.635 kb on - strand at 87.635 kb on - strand at 87.635 kb on - strand at 87.645 kb on - strand at 87.647 kb on + strand at 87.654 kb on - strand at 87.697 kb on + strand at 87.697 kb on + strand at 87.697 kb on + strand at 87.698 kb on - strand at 87.698 kb on - strand at 87.718 kb on - strand at 87.761 kb on + strand at 87.762 kb on - strand at 87.766 kb on + strand at 87.786 kb on + strand at 87.787 kb on - strand at 87.787 kb on - strand at 88.132 kb on - strand, within EX28DRAFT_4420 at 88.327 kb on + strand, within EX28DRAFT_4420 at 88.635 kb on - strand at 88.674 kb on + strand at 88.675 kb on - strand at 88.707 kb on + strand at 88.737 kb on + strand at 88.754 kb on + strand at 89.048 kb on + strand, within EX28DRAFT_4421 at 89.484 kb on + strand at 89.484 kb on + strand at 89.490 kb on - strand at 89.515 kb on + strand at 89.549 kb on + strand at 89.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 87,163 + EX28DRAFT_4419 0.60 -4.0 87,211 + EX28DRAFT_4419 0.64 -4.1 87,634 + +0.6 87,634 + +0.2 87,634 + +0.4 87,634 + -1.0 87,634 + +0.4 87,635 - -1.4 87,635 - -2.1 87,635 - -1.7 87,635 - -0.1 87,645 - -2.2 87,647 + -0.0 87,654 - -0.9 87,697 + +0.0 87,697 + +0.3 87,697 + -3.7 87,698 - -3.2 87,698 - -4.4 87,718 - -5.8 87,761 + -3.8 87,762 - -5.7 87,766 + -6.1 87,786 + -3.7 87,787 - -5.0 87,787 - -2.7 88,132 - EX28DRAFT_4420 0.42 -3.6 88,327 + EX28DRAFT_4420 0.61 -2.6 88,635 - -3.7 88,674 + -4.7 88,675 - -2.0 88,707 + -1.0 88,737 + -3.1 88,754 + -1.7 89,048 + EX28DRAFT_4421 0.42 -4.7 89,484 + -2.1 89,484 + -4.1 89,490 - -4.6 89,515 + +0.2 89,549 + +1.0 89,670 - -3.1
Or see this region's nucleotide sequence