Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_4373 and EX28DRAFT_4374 are separated by 39 nucleotides EX28DRAFT_4374 and EX28DRAFT_4375 overlap by 4 nucleotides EX28DRAFT_4375 and EX28DRAFT_4376 overlap by 4 nucleotides EX28DRAFT_4376 and EX28DRAFT_4377 are separated by 12 nucleotides
EX28DRAFT_4373: EX28DRAFT_4373 - Predicted nucleic acid-binding protein, contains PIN domain, at 38,277 to 38,648
_4373
EX28DRAFT_4374: EX28DRAFT_4374 - formate dehydrogenase accessory protein FdhE, at 38,688 to 39,617
_4374
EX28DRAFT_4375: EX28DRAFT_4375 - formate dehydrogenase, gamma subunit, at 39,614 to 40,249
_4375
EX28DRAFT_4376: EX28DRAFT_4376 - formate dehydrogenase, beta subunit, Fe-S containing, at 40,246 to 41,148
_4376
EX28DRAFT_4377: EX28DRAFT_4377 - formate dehydrogenase (quinone-dependent) catalytic subunit, at 41,161 to 44,211
_4377
Position (kb) 39
40
41 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 38.646 kb on + strand at 38.685 kb on + strand at 38.685 kb on + strand at 38.685 kb on + strand at 38.689 kb on + strand at 38.689 kb on + strand at 38.689 kb on + strand at 38.837 kb on - strand, within EX28DRAFT_4374 at 38.837 kb on - strand, within EX28DRAFT_4374 at 38.899 kb on + strand, within EX28DRAFT_4374 at 38.899 kb on + strand, within EX28DRAFT_4374 at 38.899 kb on + strand, within EX28DRAFT_4374 at 38.900 kb on - strand, within EX28DRAFT_4374 at 38.900 kb on - strand, within EX28DRAFT_4374 at 39.119 kb on - strand, within EX28DRAFT_4374 at 39.141 kb on - strand, within EX28DRAFT_4374 at 39.556 kb on + strand at 39.557 kb on - strand at 39.655 kb on - strand at 39.686 kb on - strand, within EX28DRAFT_4375 at 39.744 kb on + strand, within EX28DRAFT_4375 at 39.744 kb on + strand, within EX28DRAFT_4375 at 39.748 kb on + strand, within EX28DRAFT_4375 at 39.748 kb on + strand, within EX28DRAFT_4375 at 39.748 kb on + strand, within EX28DRAFT_4375 at 39.749 kb on - strand, within EX28DRAFT_4375 at 39.749 kb on - strand, within EX28DRAFT_4375 at 39.749 kb on - strand, within EX28DRAFT_4375 at 39.749 kb on - strand, within EX28DRAFT_4375 at 39.749 kb on - strand, within EX28DRAFT_4375 at 39.844 kb on - strand, within EX28DRAFT_4375 at 39.892 kb on + strand, within EX28DRAFT_4375 at 39.931 kb on - strand, within EX28DRAFT_4375 at 39.971 kb on - strand, within EX28DRAFT_4375 at 39.971 kb on - strand, within EX28DRAFT_4375 at 39.977 kb on - strand, within EX28DRAFT_4375 at 39.992 kb on + strand, within EX28DRAFT_4375 at 39.992 kb on + strand, within EX28DRAFT_4375 at 39.993 kb on - strand, within EX28DRAFT_4375 at 39.994 kb on + strand, within EX28DRAFT_4375 at 40.010 kb on + strand, within EX28DRAFT_4375 at 40.011 kb on - strand, within EX28DRAFT_4375 at 40.011 kb on - strand, within EX28DRAFT_4375 at 40.011 kb on - strand, within EX28DRAFT_4375 at 40.011 kb on - strand, within EX28DRAFT_4375 at 40.015 kb on + strand, within EX28DRAFT_4375 at 40.045 kb on + strand, within EX28DRAFT_4375 at 40.221 kb on + strand at 40.323 kb on - strand at 40.472 kb on + strand, within EX28DRAFT_4376 at 40.473 kb on - strand, within EX28DRAFT_4376 at 40.473 kb on - strand, within EX28DRAFT_4376 at 40.575 kb on + strand, within EX28DRAFT_4376 at 40.576 kb on - strand, within EX28DRAFT_4376 at 40.787 kb on + strand, within EX28DRAFT_4376 at 40.788 kb on - strand, within EX28DRAFT_4376 at 40.848 kb on + strand, within EX28DRAFT_4376 at 40.848 kb on + strand, within EX28DRAFT_4376 at 40.849 kb on - strand, within EX28DRAFT_4376 at 40.849 kb on - strand, within EX28DRAFT_4376 at 40.952 kb on + strand, within EX28DRAFT_4376 at 40.953 kb on - strand, within EX28DRAFT_4376 at 40.953 kb on - strand, within EX28DRAFT_4376 at 41.023 kb on - strand, within EX28DRAFT_4376 at 41.141 kb on + strand at 41.142 kb on - strand at 41.149 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 38,646 + -0.0 38,685 + -2.5 38,685 + +1.8 38,685 + +1.2 38,689 + +0.1 38,689 + -0.3 38,689 + +0.0 38,837 - EX28DRAFT_4374 0.16 -0.1 38,837 - EX28DRAFT_4374 0.16 +1.0 38,899 + EX28DRAFT_4374 0.23 -0.3 38,899 + EX28DRAFT_4374 0.23 -4.0 38,899 + EX28DRAFT_4374 0.23 -0.0 38,900 - EX28DRAFT_4374 0.23 -2.2 38,900 - EX28DRAFT_4374 0.23 +0.9 39,119 - EX28DRAFT_4374 0.46 -0.1 39,141 - EX28DRAFT_4374 0.49 +0.1 39,556 + -0.2 39,557 - +0.9 39,655 - -0.3 39,686 - EX28DRAFT_4375 0.11 -0.8 39,744 + EX28DRAFT_4375 0.20 -1.7 39,744 + EX28DRAFT_4375 0.20 -0.7 39,748 + EX28DRAFT_4375 0.21 -1.0 39,748 + EX28DRAFT_4375 0.21 -0.2 39,748 + EX28DRAFT_4375 0.21 -0.3 39,749 - EX28DRAFT_4375 0.21 -0.3 39,749 - EX28DRAFT_4375 0.21 +0.2 39,749 - EX28DRAFT_4375 0.21 +0.3 39,749 - EX28DRAFT_4375 0.21 -0.0 39,749 - EX28DRAFT_4375 0.21 +0.3 39,844 - EX28DRAFT_4375 0.36 -0.6 39,892 + EX28DRAFT_4375 0.44 +0.1 39,931 - EX28DRAFT_4375 0.50 -0.0 39,971 - EX28DRAFT_4375 0.56 -0.8 39,971 - EX28DRAFT_4375 0.56 +0.0 39,977 - EX28DRAFT_4375 0.57 -0.8 39,992 + EX28DRAFT_4375 0.59 -0.1 39,992 + EX28DRAFT_4375 0.59 +0.1 39,993 - EX28DRAFT_4375 0.60 -1.0 39,994 + EX28DRAFT_4375 0.60 -1.7 40,010 + EX28DRAFT_4375 0.62 +0.3 40,011 - EX28DRAFT_4375 0.62 +0.3 40,011 - EX28DRAFT_4375 0.62 -0.7 40,011 - EX28DRAFT_4375 0.62 +0.9 40,011 - EX28DRAFT_4375 0.62 -0.0 40,015 + EX28DRAFT_4375 0.63 +0.6 40,045 + EX28DRAFT_4375 0.68 +0.4 40,221 + -0.2 40,323 - -0.1 40,472 + EX28DRAFT_4376 0.25 +0.1 40,473 - EX28DRAFT_4376 0.25 -0.3 40,473 - EX28DRAFT_4376 0.25 -0.7 40,575 + EX28DRAFT_4376 0.36 +0.5 40,576 - EX28DRAFT_4376 0.37 -1.0 40,787 + EX28DRAFT_4376 0.60 +0.6 40,788 - EX28DRAFT_4376 0.60 +0.5 40,848 + EX28DRAFT_4376 0.67 +0.8 40,848 + EX28DRAFT_4376 0.67 +0.4 40,849 - EX28DRAFT_4376 0.67 -0.0 40,849 - EX28DRAFT_4376 0.67 +1.4 40,952 + EX28DRAFT_4376 0.78 +0.5 40,953 - EX28DRAFT_4376 0.78 -0.7 40,953 - EX28DRAFT_4376 0.78 -0.1 41,023 - EX28DRAFT_4376 0.86 -0.9 41,141 + +0.2 41,142 - -0.1 41,149 - -0.3
Or see this region's nucleotide sequence