Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3921 and EX28DRAFT_3922 are separated by 2 nucleotides EX28DRAFT_3922 and EX28DRAFT_3923 are separated by 117 nucleotides EX28DRAFT_3923 and EX28DRAFT_3924 are separated by 99 nucleotides
EX28DRAFT_3921: EX28DRAFT_3921 - P pilus assembly protein, pilin FimA, at 131,227 to 132,240
_3921
EX28DRAFT_3922: EX28DRAFT_3922 - Bacterial lipocalin, at 132,243 to 132,767
_3922
EX28DRAFT_3923: EX28DRAFT_3923 - Predicted transcriptional regulators, at 132,885 to 133,610
_3923
EX28DRAFT_3924: EX28DRAFT_3924 - hydroxyisourate hydrolase, at 133,710 to 134,120
_3924
Position (kb) 132
133 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 131.304 kb on + strand at 131.305 kb on - strand at 131.329 kb on + strand, within EX28DRAFT_3921 at 131.510 kb on - strand, within EX28DRAFT_3921 at 131.545 kb on + strand, within EX28DRAFT_3921 at 131.545 kb on + strand, within EX28DRAFT_3921 at 131.546 kb on - strand, within EX28DRAFT_3921 at 131.581 kb on + strand, within EX28DRAFT_3921 at 131.581 kb on + strand, within EX28DRAFT_3921 at 131.582 kb on - strand, within EX28DRAFT_3921 at 131.583 kb on + strand, within EX28DRAFT_3921 at 131.584 kb on - strand, within EX28DRAFT_3921 at 131.639 kb on - strand, within EX28DRAFT_3921 at 131.679 kb on + strand, within EX28DRAFT_3921 at 131.710 kb on + strand, within EX28DRAFT_3921 at 131.712 kb on + strand, within EX28DRAFT_3921 at 131.712 kb on + strand, within EX28DRAFT_3921 at 131.713 kb on - strand, within EX28DRAFT_3921 at 131.713 kb on - strand, within EX28DRAFT_3921 at 131.713 kb on - strand, within EX28DRAFT_3921 at 131.751 kb on + strand, within EX28DRAFT_3921 at 131.752 kb on - strand, within EX28DRAFT_3921 at 131.820 kb on + strand, within EX28DRAFT_3921 at 132.169 kb on + strand at 132.169 kb on + strand at 132.170 kb on - strand at 132.398 kb on + strand, within EX28DRAFT_3922 at 132.399 kb on - strand, within EX28DRAFT_3922 at 132.421 kb on + strand, within EX28DRAFT_3922 at 132.422 kb on - strand, within EX28DRAFT_3922 at 132.422 kb on - strand, within EX28DRAFT_3922 at 132.430 kb on + strand, within EX28DRAFT_3922 at 132.487 kb on + strand, within EX28DRAFT_3922 at 132.487 kb on + strand, within EX28DRAFT_3922 at 132.488 kb on - strand, within EX28DRAFT_3922 at 132.488 kb on - strand, within EX28DRAFT_3922 at 132.578 kb on - strand, within EX28DRAFT_3922 at 132.710 kb on + strand, within EX28DRAFT_3922 at 132.799 kb on + strand at 132.805 kb on + strand at 132.806 kb on - strand at 132.850 kb on + strand at 132.851 kb on - strand at 132.851 kb on - strand at 132.852 kb on + strand at 132.852 kb on + strand at 132.853 kb on - strand at 132.853 kb on - strand at 132.891 kb on + strand at 133.326 kb on + strand, within EX28DRAFT_3923 at 133.327 kb on - strand, within EX28DRAFT_3923 at 133.373 kb on + strand, within EX28DRAFT_3923 at 133.374 kb on - strand, within EX28DRAFT_3923 at 133.374 kb on - strand, within EX28DRAFT_3923 at 133.511 kb on + strand, within EX28DRAFT_3923 at 133.511 kb on + strand, within EX28DRAFT_3923 at 133.512 kb on - strand, within EX28DRAFT_3923 at 133.690 kb on + strand at 133.691 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 131,304 + +0.3 131,305 - +0.5 131,329 + EX28DRAFT_3921 0.10 +0.5 131,510 - EX28DRAFT_3921 0.28 +1.1 131,545 + EX28DRAFT_3921 0.31 +0.7 131,545 + EX28DRAFT_3921 0.31 -0.4 131,546 - EX28DRAFT_3921 0.31 -0.2 131,581 + EX28DRAFT_3921 0.35 -1.9 131,581 + EX28DRAFT_3921 0.35 -0.3 131,582 - EX28DRAFT_3921 0.35 +1.5 131,583 + EX28DRAFT_3921 0.35 -2.4 131,584 - EX28DRAFT_3921 0.35 -2.2 131,639 - EX28DRAFT_3921 0.41 -1.6 131,679 + EX28DRAFT_3921 0.45 +0.2 131,710 + EX28DRAFT_3921 0.48 -1.6 131,712 + EX28DRAFT_3921 0.48 +0.4 131,712 + EX28DRAFT_3921 0.48 +0.4 131,713 - EX28DRAFT_3921 0.48 -0.3 131,713 - EX28DRAFT_3921 0.48 +0.3 131,713 - EX28DRAFT_3921 0.48 -0.2 131,751 + EX28DRAFT_3921 0.52 -2.2 131,752 - EX28DRAFT_3921 0.52 -2.8 131,820 + EX28DRAFT_3921 0.58 +0.2 132,169 + -0.3 132,169 + +0.4 132,170 - +0.0 132,398 + EX28DRAFT_3922 0.30 -0.7 132,399 - EX28DRAFT_3922 0.30 -0.2 132,421 + EX28DRAFT_3922 0.34 +2.4 132,422 - EX28DRAFT_3922 0.34 +0.9 132,422 - EX28DRAFT_3922 0.34 +0.2 132,430 + EX28DRAFT_3922 0.36 +0.3 132,487 + EX28DRAFT_3922 0.46 +0.7 132,487 + EX28DRAFT_3922 0.46 +0.6 132,488 - EX28DRAFT_3922 0.47 -0.7 132,488 - EX28DRAFT_3922 0.47 -0.3 132,578 - EX28DRAFT_3922 0.64 +0.8 132,710 + EX28DRAFT_3922 0.89 +0.4 132,799 + +0.3 132,805 + +1.5 132,806 - -0.8 132,850 + +0.2 132,851 - -0.3 132,851 - +0.6 132,852 + +0.0 132,852 + +2.2 132,853 - -0.1 132,853 - +0.3 132,891 + +0.1 133,326 + EX28DRAFT_3923 0.61 -1.5 133,327 - EX28DRAFT_3923 0.61 -0.8 133,373 + EX28DRAFT_3923 0.67 +0.6 133,374 - EX28DRAFT_3923 0.67 -0.7 133,374 - EX28DRAFT_3923 0.67 -0.3 133,511 + EX28DRAFT_3923 0.86 +0.4 133,511 + EX28DRAFT_3923 0.86 -2.1 133,512 - EX28DRAFT_3923 0.86 +0.8 133,690 + +0.2 133,691 - +2.2
Or see this region's nucleotide sequence