Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3919 and EX28DRAFT_3920 overlap by 10 nucleotides EX28DRAFT_3920 and EX28DRAFT_3921 overlap by 4 nucleotides EX28DRAFT_3921 and EX28DRAFT_3922 are separated by 2 nucleotides EX28DRAFT_3922 and EX28DRAFT_3923 are separated by 117 nucleotides
EX28DRAFT_3919: EX28DRAFT_3919 - P pilus assembly protein, porin PapC, at 128,199 to 130,724
_3919
EX28DRAFT_3920: EX28DRAFT_3920 - P pilus assembly protein, pilin FimA, at 130,715 to 131,230
_3920
EX28DRAFT_3921: EX28DRAFT_3921 - P pilus assembly protein, pilin FimA, at 131,227 to 132,240
_3921
EX28DRAFT_3922: EX28DRAFT_3922 - Bacterial lipocalin, at 132,243 to 132,767
_3922
EX28DRAFT_3923: EX28DRAFT_3923 - Predicted transcriptional regulators, at 132,885 to 133,610
_3923
Position (kb) 131
132
133 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 130.229 kb on + strand, within EX28DRAFT_3919 at 130.481 kb on + strand at 130.653 kb on - strand at 130.656 kb on + strand at 130.657 kb on - strand at 130.664 kb on - strand at 130.714 kb on + strand at 130.714 kb on + strand at 130.715 kb on - strand at 130.715 kb on - strand at 130.715 kb on - strand at 130.888 kb on + strand, within EX28DRAFT_3920 at 130.912 kb on + strand, within EX28DRAFT_3920 at 130.913 kb on - strand, within EX28DRAFT_3920 at 131.077 kb on - strand, within EX28DRAFT_3920 at 131.093 kb on - strand, within EX28DRAFT_3920 at 131.133 kb on + strand, within EX28DRAFT_3920 at 131.134 kb on - strand, within EX28DRAFT_3920 at 131.134 kb on - strand, within EX28DRAFT_3920 at 131.222 kb on + strand at 131.222 kb on + strand at 131.223 kb on - strand at 131.226 kb on + strand at 131.226 kb on + strand at 131.226 kb on + strand at 131.226 kb on + strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.304 kb on + strand at 131.305 kb on - strand at 131.329 kb on + strand, within EX28DRAFT_3921 at 131.510 kb on - strand, within EX28DRAFT_3921 at 131.545 kb on + strand, within EX28DRAFT_3921 at 131.545 kb on + strand, within EX28DRAFT_3921 at 131.546 kb on - strand, within EX28DRAFT_3921 at 131.581 kb on + strand, within EX28DRAFT_3921 at 131.581 kb on + strand, within EX28DRAFT_3921 at 131.582 kb on - strand, within EX28DRAFT_3921 at 131.583 kb on + strand, within EX28DRAFT_3921 at 131.584 kb on - strand, within EX28DRAFT_3921 at 131.639 kb on - strand, within EX28DRAFT_3921 at 131.679 kb on + strand, within EX28DRAFT_3921 at 131.710 kb on + strand, within EX28DRAFT_3921 at 131.712 kb on + strand, within EX28DRAFT_3921 at 131.712 kb on + strand, within EX28DRAFT_3921 at 131.713 kb on - strand, within EX28DRAFT_3921 at 131.713 kb on - strand, within EX28DRAFT_3921 at 131.713 kb on - strand, within EX28DRAFT_3921 at 131.751 kb on + strand, within EX28DRAFT_3921 at 131.752 kb on - strand, within EX28DRAFT_3921 at 131.820 kb on + strand, within EX28DRAFT_3921 at 132.169 kb on + strand at 132.169 kb on + strand at 132.170 kb on - strand at 132.398 kb on + strand, within EX28DRAFT_3922 at 132.399 kb on - strand, within EX28DRAFT_3922 at 132.421 kb on + strand, within EX28DRAFT_3922 at 132.422 kb on - strand, within EX28DRAFT_3922 at 132.422 kb on - strand, within EX28DRAFT_3922 at 132.430 kb on + strand, within EX28DRAFT_3922 at 132.487 kb on + strand, within EX28DRAFT_3922 at 132.487 kb on + strand, within EX28DRAFT_3922 at 132.488 kb on - strand, within EX28DRAFT_3922 at 132.488 kb on - strand, within EX28DRAFT_3922 at 132.578 kb on - strand, within EX28DRAFT_3922 at 132.710 kb on + strand, within EX28DRAFT_3922 at 132.799 kb on + strand at 132.805 kb on + strand at 132.806 kb on - strand at 132.850 kb on + strand at 132.851 kb on - strand at 132.851 kb on - strand at 132.852 kb on + strand at 132.852 kb on + strand at 132.853 kb on - strand at 132.853 kb on - strand at 132.891 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 130,229 + EX28DRAFT_3919 0.80 +0.2 130,481 + -0.8 130,653 - +0.5 130,656 + +0.2 130,657 - +0.0 130,664 - -0.6 130,714 + -1.9 130,714 + +0.1 130,715 - +0.5 130,715 - -0.3 130,715 - +0.4 130,888 + EX28DRAFT_3920 0.34 +1.1 130,912 + EX28DRAFT_3920 0.38 +0.9 130,913 - EX28DRAFT_3920 0.38 +1.3 131,077 - EX28DRAFT_3920 0.70 -0.5 131,093 - EX28DRAFT_3920 0.73 +0.8 131,133 + EX28DRAFT_3920 0.81 +0.3 131,134 - EX28DRAFT_3920 0.81 -0.2 131,134 - EX28DRAFT_3920 0.81 +0.5 131,222 + +0.7 131,222 + +0.5 131,223 - +0.1 131,226 + +1.0 131,226 + +0.1 131,226 + +1.3 131,226 + +0.4 131,227 - +1.4 131,227 - +1.2 131,227 - -0.0 131,227 - +0.4 131,227 - +0.1 131,227 - +0.4 131,227 - -0.2 131,227 - +1.4 131,227 - -1.2 131,227 - +0.1 131,304 + +0.3 131,305 - +0.5 131,329 + EX28DRAFT_3921 0.10 +0.5 131,510 - EX28DRAFT_3921 0.28 +1.1 131,545 + EX28DRAFT_3921 0.31 +0.7 131,545 + EX28DRAFT_3921 0.31 -0.4 131,546 - EX28DRAFT_3921 0.31 -0.2 131,581 + EX28DRAFT_3921 0.35 -1.9 131,581 + EX28DRAFT_3921 0.35 -0.3 131,582 - EX28DRAFT_3921 0.35 +1.5 131,583 + EX28DRAFT_3921 0.35 -2.4 131,584 - EX28DRAFT_3921 0.35 -2.2 131,639 - EX28DRAFT_3921 0.41 -1.6 131,679 + EX28DRAFT_3921 0.45 +0.2 131,710 + EX28DRAFT_3921 0.48 -1.6 131,712 + EX28DRAFT_3921 0.48 +0.4 131,712 + EX28DRAFT_3921 0.48 +0.4 131,713 - EX28DRAFT_3921 0.48 -0.3 131,713 - EX28DRAFT_3921 0.48 +0.3 131,713 - EX28DRAFT_3921 0.48 -0.2 131,751 + EX28DRAFT_3921 0.52 -2.2 131,752 - EX28DRAFT_3921 0.52 -2.8 131,820 + EX28DRAFT_3921 0.58 +0.2 132,169 + -0.3 132,169 + +0.4 132,170 - +0.0 132,398 + EX28DRAFT_3922 0.30 -0.7 132,399 - EX28DRAFT_3922 0.30 -0.2 132,421 + EX28DRAFT_3922 0.34 +2.4 132,422 - EX28DRAFT_3922 0.34 +0.9 132,422 - EX28DRAFT_3922 0.34 +0.2 132,430 + EX28DRAFT_3922 0.36 +0.3 132,487 + EX28DRAFT_3922 0.46 +0.7 132,487 + EX28DRAFT_3922 0.46 +0.6 132,488 - EX28DRAFT_3922 0.47 -0.7 132,488 - EX28DRAFT_3922 0.47 -0.3 132,578 - EX28DRAFT_3922 0.64 +0.8 132,710 + EX28DRAFT_3922 0.89 +0.4 132,799 + +0.3 132,805 + +1.5 132,806 - -0.8 132,850 + +0.2 132,851 - -0.3 132,851 - +0.6 132,852 + +0.0 132,852 + +2.2 132,853 - -0.1 132,853 - +0.3 132,891 + +0.1
Or see this region's nucleotide sequence