Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3917 and EX28DRAFT_3918 are separated by 53 nucleotides EX28DRAFT_3918 and EX28DRAFT_3919 are separated by 23 nucleotides EX28DRAFT_3919 and EX28DRAFT_3920 overlap by 10 nucleotides EX28DRAFT_3920 and EX28DRAFT_3921 overlap by 4 nucleotides
EX28DRAFT_3917: EX28DRAFT_3917 - P pilus assembly protein, pilin FimA, at 126,860 to 127,444
_3917
EX28DRAFT_3918: EX28DRAFT_3918 - P pilus assembly protein, chaperone PapD, at 127,498 to 128,175
_3918
EX28DRAFT_3919: EX28DRAFT_3919 - P pilus assembly protein, porin PapC, at 128,199 to 130,724
_3919
EX28DRAFT_3920: EX28DRAFT_3920 - P pilus assembly protein, pilin FimA, at 130,715 to 131,230
_3920
EX28DRAFT_3921: EX28DRAFT_3921 - P pilus assembly protein, pilin FimA, at 131,227 to 132,240
_3921
Position (kb) 128
129
130
131 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 127.213 kb on + strand, within EX28DRAFT_3917 at 127.214 kb on - strand, within EX28DRAFT_3917 at 127.214 kb on - strand, within EX28DRAFT_3917 at 127.216 kb on + strand, within EX28DRAFT_3917 at 127.217 kb on - strand, within EX28DRAFT_3917 at 127.217 kb on - strand, within EX28DRAFT_3917 at 127.277 kb on + strand, within EX28DRAFT_3917 at 127.337 kb on - strand, within EX28DRAFT_3917 at 127.371 kb on - strand, within EX28DRAFT_3917 at 127.371 kb on - strand, within EX28DRAFT_3917 at 127.424 kb on + strand at 127.425 kb on - strand at 127.436 kb on + strand at 127.437 kb on - strand at 127.443 kb on - strand at 127.478 kb on + strand at 127.497 kb on + strand at 127.498 kb on - strand at 127.498 kb on - strand at 127.536 kb on - strand at 127.724 kb on - strand, within EX28DRAFT_3918 at 127.804 kb on + strand, within EX28DRAFT_3918 at 127.897 kb on + strand, within EX28DRAFT_3918 at 127.999 kb on + strand, within EX28DRAFT_3918 at 128.000 kb on - strand, within EX28DRAFT_3918 at 128.000 kb on - strand, within EX28DRAFT_3918 at 128.364 kb on + strand at 128.364 kb on + strand at 128.379 kb on + strand at 128.379 kb on + strand at 128.379 kb on + strand at 128.380 kb on - strand at 128.380 kb on - strand at 128.380 kb on - strand at 128.381 kb on + strand at 128.382 kb on - strand at 128.395 kb on - strand at 128.548 kb on + strand, within EX28DRAFT_3919 at 128.701 kb on - strand, within EX28DRAFT_3919 at 128.901 kb on + strand, within EX28DRAFT_3919 at 128.902 kb on - strand, within EX28DRAFT_3919 at 128.916 kb on + strand, within EX28DRAFT_3919 at 128.916 kb on + strand, within EX28DRAFT_3919 at 128.917 kb on - strand, within EX28DRAFT_3919 at 128.917 kb on - strand, within EX28DRAFT_3919 at 128.917 kb on - strand, within EX28DRAFT_3919 at 129.007 kb on - strand, within EX28DRAFT_3919 at 129.078 kb on + strand, within EX28DRAFT_3919 at 129.244 kb on - strand, within EX28DRAFT_3919 at 129.244 kb on - strand, within EX28DRAFT_3919 at 129.244 kb on - strand, within EX28DRAFT_3919 at 129.264 kb on + strand, within EX28DRAFT_3919 at 129.318 kb on + strand, within EX28DRAFT_3919 at 129.423 kb on + strand, within EX28DRAFT_3919 at 129.502 kb on - strand, within EX28DRAFT_3919 at 129.573 kb on + strand, within EX28DRAFT_3919 at 129.574 kb on - strand, within EX28DRAFT_3919 at 129.574 kb on - strand, within EX28DRAFT_3919 at 129.574 kb on - strand, within EX28DRAFT_3919 at 129.575 kb on + strand, within EX28DRAFT_3919 at 129.594 kb on + strand, within EX28DRAFT_3919 at 129.594 kb on + strand, within EX28DRAFT_3919 at 129.595 kb on - strand, within EX28DRAFT_3919 at 129.595 kb on - strand, within EX28DRAFT_3919 at 129.595 kb on - strand, within EX28DRAFT_3919 at 129.595 kb on - strand, within EX28DRAFT_3919 at 129.660 kb on + strand, within EX28DRAFT_3919 at 129.672 kb on + strand, within EX28DRAFT_3919 at 129.672 kb on + strand, within EX28DRAFT_3919 at 129.673 kb on - strand, within EX28DRAFT_3919 at 129.673 kb on - strand, within EX28DRAFT_3919 at 129.736 kb on - strand, within EX28DRAFT_3919 at 129.789 kb on - strand, within EX28DRAFT_3919 at 129.890 kb on + strand, within EX28DRAFT_3919 at 129.891 kb on - strand, within EX28DRAFT_3919 at 130.038 kb on + strand, within EX28DRAFT_3919 at 130.039 kb on - strand, within EX28DRAFT_3919 at 130.099 kb on - strand, within EX28DRAFT_3919 at 130.120 kb on - strand, within EX28DRAFT_3919 at 130.165 kb on - strand, within EX28DRAFT_3919 at 130.229 kb on + strand, within EX28DRAFT_3919 at 130.481 kb on + strand at 130.653 kb on - strand at 130.656 kb on + strand at 130.657 kb on - strand at 130.664 kb on - strand at 130.714 kb on + strand at 130.714 kb on + strand at 130.715 kb on - strand at 130.715 kb on - strand at 130.715 kb on - strand at 130.888 kb on + strand, within EX28DRAFT_3920 at 130.912 kb on + strand, within EX28DRAFT_3920 at 130.913 kb on - strand, within EX28DRAFT_3920 at 131.077 kb on - strand, within EX28DRAFT_3920 at 131.093 kb on - strand, within EX28DRAFT_3920 at 131.133 kb on + strand, within EX28DRAFT_3920 at 131.134 kb on - strand, within EX28DRAFT_3920 at 131.134 kb on - strand, within EX28DRAFT_3920 at 131.222 kb on + strand at 131.222 kb on + strand at 131.223 kb on - strand at 131.226 kb on + strand at 131.226 kb on + strand at 131.226 kb on + strand at 131.226 kb on + strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.227 kb on - strand at 131.304 kb on + strand at 131.305 kb on - strand at 131.329 kb on + strand, within EX28DRAFT_3921 at 131.510 kb on - strand, within EX28DRAFT_3921 at 131.545 kb on + strand, within EX28DRAFT_3921 at 131.545 kb on + strand, within EX28DRAFT_3921 at 131.546 kb on - strand, within EX28DRAFT_3921 at 131.581 kb on + strand, within EX28DRAFT_3921 at 131.581 kb on + strand, within EX28DRAFT_3921 at 131.582 kb on - strand, within EX28DRAFT_3921 at 131.583 kb on + strand, within EX28DRAFT_3921 at 131.584 kb on - strand, within EX28DRAFT_3921 at 131.639 kb on - strand, within EX28DRAFT_3921 at 131.679 kb on + strand, within EX28DRAFT_3921 at 131.710 kb on + strand, within EX28DRAFT_3921 at 131.712 kb on + strand, within EX28DRAFT_3921 at 131.712 kb on + strand, within EX28DRAFT_3921 at 131.713 kb on - strand, within EX28DRAFT_3921 at 131.713 kb on - strand, within EX28DRAFT_3921 at 131.713 kb on - strand, within EX28DRAFT_3921
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 127,213 + EX28DRAFT_3917 0.60 +0.1 127,214 - EX28DRAFT_3917 0.61 -0.8 127,214 - EX28DRAFT_3917 0.61 -0.4 127,216 + EX28DRAFT_3917 0.61 +1.4 127,217 - EX28DRAFT_3917 0.61 -0.1 127,217 - EX28DRAFT_3917 0.61 -0.3 127,277 + EX28DRAFT_3917 0.71 -0.2 127,337 - EX28DRAFT_3917 0.82 +0.6 127,371 - EX28DRAFT_3917 0.87 +0.2 127,371 - EX28DRAFT_3917 0.87 -0.1 127,424 + -0.1 127,425 - -0.6 127,436 + -0.6 127,437 - -0.4 127,443 - +0.3 127,478 + -0.0 127,497 + -0.3 127,498 - +0.3 127,498 - +0.2 127,536 - -0.3 127,724 - EX28DRAFT_3918 0.33 +0.1 127,804 + EX28DRAFT_3918 0.45 +0.7 127,897 + EX28DRAFT_3918 0.59 +0.4 127,999 + EX28DRAFT_3918 0.74 +0.4 128,000 - EX28DRAFT_3918 0.74 +0.1 128,000 - EX28DRAFT_3918 0.74 -0.1 128,364 + +0.4 128,364 + -0.6 128,379 + -0.0 128,379 + -1.9 128,379 + +0.2 128,380 - -0.7 128,380 - +1.0 128,380 - +0.5 128,381 + -0.2 128,382 - +0.9 128,395 - -0.4 128,548 + EX28DRAFT_3919 0.14 +0.3 128,701 - EX28DRAFT_3919 0.20 +0.4 128,901 + EX28DRAFT_3919 0.28 -0.4 128,902 - EX28DRAFT_3919 0.28 +0.1 128,916 + EX28DRAFT_3919 0.28 +1.1 128,916 + EX28DRAFT_3919 0.28 +0.6 128,917 - EX28DRAFT_3919 0.28 +0.7 128,917 - EX28DRAFT_3919 0.28 +0.3 128,917 - EX28DRAFT_3919 0.28 +0.2 129,007 - EX28DRAFT_3919 0.32 +0.3 129,078 + EX28DRAFT_3919 0.35 +0.6 129,244 - EX28DRAFT_3919 0.41 +0.4 129,244 - EX28DRAFT_3919 0.41 +0.2 129,244 - EX28DRAFT_3919 0.41 -0.6 129,264 + EX28DRAFT_3919 0.42 -0.2 129,318 + EX28DRAFT_3919 0.44 +1.4 129,423 + EX28DRAFT_3919 0.48 -1.4 129,502 - EX28DRAFT_3919 0.52 -3.9 129,573 + EX28DRAFT_3919 0.54 +0.2 129,574 - EX28DRAFT_3919 0.54 +0.9 129,574 - EX28DRAFT_3919 0.54 +0.8 129,574 - EX28DRAFT_3919 0.54 +0.6 129,575 + EX28DRAFT_3919 0.54 -0.3 129,594 + EX28DRAFT_3919 0.55 +0.7 129,594 + EX28DRAFT_3919 0.55 +0.0 129,595 - EX28DRAFT_3919 0.55 +0.3 129,595 - EX28DRAFT_3919 0.55 +0.1 129,595 - EX28DRAFT_3919 0.55 -0.1 129,595 - EX28DRAFT_3919 0.55 -0.5 129,660 + EX28DRAFT_3919 0.58 +0.6 129,672 + EX28DRAFT_3919 0.58 +0.6 129,672 + EX28DRAFT_3919 0.58 -1.8 129,673 - EX28DRAFT_3919 0.58 -2.2 129,673 - EX28DRAFT_3919 0.58 -0.0 129,736 - EX28DRAFT_3919 0.61 +0.1 129,789 - EX28DRAFT_3919 0.63 +0.2 129,890 + EX28DRAFT_3919 0.67 +0.5 129,891 - EX28DRAFT_3919 0.67 +0.3 130,038 + EX28DRAFT_3919 0.73 +0.3 130,039 - EX28DRAFT_3919 0.73 +0.3 130,099 - EX28DRAFT_3919 0.75 +1.4 130,120 - EX28DRAFT_3919 0.76 -0.6 130,165 - EX28DRAFT_3919 0.78 -0.7 130,229 + EX28DRAFT_3919 0.80 +0.2 130,481 + -0.8 130,653 - +0.5 130,656 + +0.2 130,657 - +0.0 130,664 - -0.6 130,714 + -1.9 130,714 + +0.1 130,715 - +0.5 130,715 - -0.3 130,715 - +0.4 130,888 + EX28DRAFT_3920 0.34 +1.1 130,912 + EX28DRAFT_3920 0.38 +0.9 130,913 - EX28DRAFT_3920 0.38 +1.3 131,077 - EX28DRAFT_3920 0.70 -0.5 131,093 - EX28DRAFT_3920 0.73 +0.8 131,133 + EX28DRAFT_3920 0.81 +0.3 131,134 - EX28DRAFT_3920 0.81 -0.2 131,134 - EX28DRAFT_3920 0.81 +0.5 131,222 + +0.7 131,222 + +0.5 131,223 - +0.1 131,226 + +1.0 131,226 + +0.1 131,226 + +1.3 131,226 + +0.4 131,227 - +1.4 131,227 - +1.2 131,227 - -0.0 131,227 - +0.4 131,227 - +0.1 131,227 - +0.4 131,227 - -0.2 131,227 - +1.4 131,227 - -1.2 131,227 - +0.1 131,304 + +0.3 131,305 - +0.5 131,329 + EX28DRAFT_3921 0.10 +0.5 131,510 - EX28DRAFT_3921 0.28 +1.1 131,545 + EX28DRAFT_3921 0.31 +0.7 131,545 + EX28DRAFT_3921 0.31 -0.4 131,546 - EX28DRAFT_3921 0.31 -0.2 131,581 + EX28DRAFT_3921 0.35 -1.9 131,581 + EX28DRAFT_3921 0.35 -0.3 131,582 - EX28DRAFT_3921 0.35 +1.5 131,583 + EX28DRAFT_3921 0.35 -2.4 131,584 - EX28DRAFT_3921 0.35 -2.2 131,639 - EX28DRAFT_3921 0.41 -1.6 131,679 + EX28DRAFT_3921 0.45 +0.2 131,710 + EX28DRAFT_3921 0.48 -1.6 131,712 + EX28DRAFT_3921 0.48 +0.4 131,712 + EX28DRAFT_3921 0.48 +0.4 131,713 - EX28DRAFT_3921 0.48 -0.3 131,713 - EX28DRAFT_3921 0.48 +0.3 131,713 - EX28DRAFT_3921 0.48 -0.2
Or see this region's nucleotide sequence