Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3896 and EX28DRAFT_3897 are separated by 251 nucleotides EX28DRAFT_3897 and EX28DRAFT_3898 are separated by 54 nucleotides EX28DRAFT_3898 and EX28DRAFT_3899 are separated by 19 nucleotides
EX28DRAFT_3896: EX28DRAFT_3896 - NAD-dependent DNA ligase (contains BRCT domain type II), at 103,222 to 104,892
_3896
EX28DRAFT_3897: EX28DRAFT_3897 - guanylate kinase (EC 2.7.4.8), at 105,144 to 105,767
_3897
EX28DRAFT_3898: EX28DRAFT_3898 - DNA-directed RNA polymerase subunit omega (EC 2.7.7.6), at 105,822 to 106,097
_3898
EX28DRAFT_3899: EX28DRAFT_3899 - (p)ppGpp synthetase, RelA/SpoT family, at 106,117 to 108,231
_3899
Position (kb) 105
106
107 Strain fitness (log2 ratio) -2
-1
0
1 at 104.908 kb on + strand at 104.922 kb on - strand at 105.012 kb on - strand at 105.071 kb on - strand at 105.097 kb on + strand at 105.097 kb on + strand at 105.105 kb on + strand at 105.817 kb on + strand at 105.821 kb on + strand at 105.965 kb on + strand, within EX28DRAFT_3898
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 104,908 + -0.0 104,922 - -0.3 105,012 - -0.1 105,071 - +0.2 105,097 + -1.6 105,097 + +1.4 105,105 + -2.2 105,817 + -0.4 105,821 + +1.4 105,965 + EX28DRAFT_3898 0.52 -0.8
Or see this region's nucleotide sequence