Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3870 and EX28DRAFT_3871 are separated by 215 nucleotides EX28DRAFT_3871 and EX28DRAFT_3872 are separated by 2 nucleotides
EX28DRAFT_3870: EX28DRAFT_3870 - 2-amino-3-ketobutyrate coenzyme A ligase (EC 2.3.1.29), at 79,573 to 80,769
_3870
EX28DRAFT_3871: EX28DRAFT_3871 - ADP-L-glycero-D-manno-heptose-6-epimerase, at 80,985 to 81,917
_3871
EX28DRAFT_3872: EX28DRAFT_3872 - lipopolysaccharide heptosyltransferase II, at 81,920 to 82,966
_3872
Position (kb) 80
81
82 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 80.162 kb on + strand, within EX28DRAFT_3870 at 80.163 kb on - strand, within EX28DRAFT_3870 at 80.331 kb on + strand, within EX28DRAFT_3870 at 80.341 kb on + strand, within EX28DRAFT_3870 at 80.615 kb on + strand, within EX28DRAFT_3870 at 80.616 kb on - strand, within EX28DRAFT_3870 at 80.688 kb on + strand at 80.691 kb on + strand at 80.691 kb on + strand at 80.692 kb on - strand at 80.803 kb on + strand at 80.804 kb on - strand at 80.804 kb on - strand at 80.816 kb on + strand at 80.816 kb on + strand at 80.816 kb on + strand at 80.818 kb on + strand at 80.819 kb on - strand at 80.864 kb on + strand at 80.915 kb on + strand at 80.915 kb on + strand at 80.915 kb on + strand at 80.915 kb on + strand at 80.916 kb on - strand at 80.916 kb on - strand at 80.919 kb on - strand at 80.919 kb on - strand at 80.924 kb on + strand at 80.925 kb on - strand at 81.915 kb on + strand at 81.919 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 80,162 + EX28DRAFT_3870 0.49 +0.0 80,163 - EX28DRAFT_3870 0.49 +0.8 80,331 + EX28DRAFT_3870 0.63 -1.9 80,341 + EX28DRAFT_3870 0.64 +1.2 80,615 + EX28DRAFT_3870 0.87 -0.8 80,616 - EX28DRAFT_3870 0.87 -1.4 80,688 + -1.3 80,691 + -0.6 80,691 + -0.6 80,692 - +1.2 80,803 + -0.4 80,804 - +0.7 80,804 - +0.2 80,816 + +2.3 80,816 + +0.7 80,816 + +0.3 80,818 + +0.1 80,819 - -1.9 80,864 + +0.6 80,915 + -0.3 80,915 + +0.4 80,915 + -0.0 80,915 + -0.7 80,916 - +1.0 80,916 - -2.6 80,919 - -1.9 80,919 - -0.1 80,924 + -0.4 80,925 - -3.1 81,915 + -0.2 81,919 + +0.9
Or see this region's nucleotide sequence