Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3743 and EX28DRAFT_3744 are separated by 177 nucleotides EX28DRAFT_3744 and EX28DRAFT_3745 are separated by 79 nucleotides EX28DRAFT_3745 and EX28DRAFT_3746 are separated by 71 nucleotides
EX28DRAFT_3743: EX28DRAFT_3743 - glutamate-1-semialdehyde-2,1-aminomutase, at 422,330 to 423,610
_3743
EX28DRAFT_3744: EX28DRAFT_3744 - Chloride channel protein EriC, at 423,788 to 425,188
_3744
EX28DRAFT_3745: EX28DRAFT_3745 - Iron-sulfur cluster assembly accessory protein, at 425,268 to 425,615
_3745
EX28DRAFT_3746: EX28DRAFT_3746 - Predicted membrane protein, at 425,687 to 426,310
_3746
Position (kb) 423
424
425
426 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 423.655 kb on - strand at 423.710 kb on + strand at 423.714 kb on + strand at 424.068 kb on - strand, within EX28DRAFT_3744 at 424.312 kb on + strand, within EX28DRAFT_3744 at 424.432 kb on + strand, within EX28DRAFT_3744 at 424.457 kb on - strand, within EX28DRAFT_3744 at 424.594 kb on + strand, within EX28DRAFT_3744 at 424.792 kb on + strand, within EX28DRAFT_3744 at 424.793 kb on - strand, within EX28DRAFT_3744 at 424.793 kb on - strand, within EX28DRAFT_3744 at 424.799 kb on - strand, within EX28DRAFT_3744 at 425.042 kb on + strand, within EX28DRAFT_3744 at 425.042 kb on + strand, within EX28DRAFT_3744 at 425.043 kb on - strand, within EX28DRAFT_3744 at 425.043 kb on - strand, within EX28DRAFT_3744 at 425.065 kb on - strand at 425.068 kb on + strand at 425.069 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.089 kb on - strand at 425.091 kb on + strand at 425.092 kb on - strand at 425.120 kb on + strand at 425.140 kb on + strand at 425.141 kb on - strand at 425.183 kb on - strand at 425.206 kb on + strand at 425.614 kb on - strand at 425.632 kb on - strand at 425.753 kb on + strand, within EX28DRAFT_3746 at 425.754 kb on - strand, within EX28DRAFT_3746 at 425.815 kb on - strand, within EX28DRAFT_3746 at 425.815 kb on - strand, within EX28DRAFT_3746 at 425.913 kb on - strand, within EX28DRAFT_3746
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 423,655 - +0.3 423,710 + +0.9 423,714 + +0.3 424,068 - EX28DRAFT_3744 0.20 +0.6 424,312 + EX28DRAFT_3744 0.37 -0.2 424,432 + EX28DRAFT_3744 0.46 +0.9 424,457 - EX28DRAFT_3744 0.48 +0.5 424,594 + EX28DRAFT_3744 0.58 +0.3 424,792 + EX28DRAFT_3744 0.72 +1.2 424,793 - EX28DRAFT_3744 0.72 +1.5 424,793 - EX28DRAFT_3744 0.72 +0.7 424,799 - EX28DRAFT_3744 0.72 -0.6 425,042 + EX28DRAFT_3744 0.90 -1.6 425,042 + EX28DRAFT_3744 0.90 -0.6 425,043 - EX28DRAFT_3744 0.90 +1.8 425,043 - EX28DRAFT_3744 0.90 -1.0 425,065 - -0.1 425,068 + +1.7 425,069 - -3.0 425,089 - +0.3 425,089 - -1.0 425,089 - -0.4 425,089 - -0.0 425,089 - -0.3 425,089 - -0.0 425,089 - +1.0 425,089 - -0.4 425,091 + -0.4 425,092 - +1.2 425,120 + +0.4 425,140 + +1.6 425,141 - -0.0 425,183 - +0.6 425,206 + +0.5 425,614 - -1.7 425,632 - +0.5 425,753 + EX28DRAFT_3746 0.11 -0.4 425,754 - EX28DRAFT_3746 0.11 +0.3 425,815 - EX28DRAFT_3746 0.21 +1.3 425,815 - EX28DRAFT_3746 0.21 +1.7 425,913 - EX28DRAFT_3746 0.36 +0.1
Or see this region's nucleotide sequence