Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3728 and EX28DRAFT_3729 are separated by 33 nucleotides EX28DRAFT_3729 and EX28DRAFT_3730 are separated by 11 nucleotides EX28DRAFT_3730 and EX28DRAFT_3731 are separated by 123 nucleotides EX28DRAFT_3731 and EX28DRAFT_3732 overlap by 4 nucleotides
EX28DRAFT_3728: EX28DRAFT_3728 - L-aspartate 1-decarboxylase (EC 4.1.1.11), at 403,913 to 404,293
_3728
EX28DRAFT_3729: EX28DRAFT_3729 - pantoate--beta-alanine ligase, at 404,327 to 405,178
_3729
EX28DRAFT_3730: EX28DRAFT_3730 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 405,190 to 405,981
_3730
EX28DRAFT_3731: EX28DRAFT_3731 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase, at 406,105 to 406,584
_3731
EX28DRAFT_3732: EX28DRAFT_3732 - poly(A) polymerase, at 406,581 to 407,945
_3732
Position (kb) 405
406 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 404.230 kb on - strand, within EX28DRAFT_3728 at 404.269 kb on + strand at 404.269 kb on + strand at 404.270 kb on - strand at 404.270 kb on - strand at 404.301 kb on - strand at 404.329 kb on - strand at 404.357 kb on + strand at 404.503 kb on + strand, within EX28DRAFT_3729 at 404.504 kb on - strand, within EX28DRAFT_3729 at 404.646 kb on - strand, within EX28DRAFT_3729 at 404.856 kb on + strand, within EX28DRAFT_3729 at 404.856 kb on + strand, within EX28DRAFT_3729 at 404.857 kb on - strand, within EX28DRAFT_3729 at 405.016 kb on + strand, within EX28DRAFT_3729 at 405.017 kb on - strand, within EX28DRAFT_3729 at 405.031 kb on + strand, within EX28DRAFT_3729 at 405.068 kb on - strand, within EX28DRAFT_3729 at 405.110 kb on - strand at 405.110 kb on - strand at 405.116 kb on - strand at 405.243 kb on + strand at 405.243 kb on + strand at 405.244 kb on - strand at 405.245 kb on + strand at 405.246 kb on - strand at 405.246 kb on - strand at 405.414 kb on + strand, within EX28DRAFT_3730 at 405.531 kb on + strand, within EX28DRAFT_3730 at 405.531 kb on + strand, within EX28DRAFT_3730 at 405.531 kb on + strand, within EX28DRAFT_3730 at 405.531 kb on + strand, within EX28DRAFT_3730 at 405.531 kb on + strand, within EX28DRAFT_3730 at 405.534 kb on - strand, within EX28DRAFT_3730 at 405.778 kb on - strand, within EX28DRAFT_3730 at 405.902 kb on + strand at 405.903 kb on - strand at 405.909 kb on - strand at 405.959 kb on - strand at 405.981 kb on + strand at 406.029 kb on - strand at 406.052 kb on + strand at 406.052 kb on + strand at 406.052 kb on + strand at 406.053 kb on - strand at 406.053 kb on - strand at 406.056 kb on + strand at 406.955 kb on - strand, within EX28DRAFT_3732 at 406.955 kb on - strand, within EX28DRAFT_3732 at 406.955 kb on - strand, within EX28DRAFT_3732
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 404,230 - EX28DRAFT_3728 0.83 -1.3 404,269 + -0.9 404,269 + -0.0 404,270 - -1.4 404,270 - -0.5 404,301 - +1.4 404,329 - +0.1 404,357 + -1.0 404,503 + EX28DRAFT_3729 0.21 +2.5 404,504 - EX28DRAFT_3729 0.21 -1.0 404,646 - EX28DRAFT_3729 0.37 +0.2 404,856 + EX28DRAFT_3729 0.62 -1.5 404,856 + EX28DRAFT_3729 0.62 -1.3 404,857 - EX28DRAFT_3729 0.62 -1.0 405,016 + EX28DRAFT_3729 0.81 -0.0 405,017 - EX28DRAFT_3729 0.81 +0.1 405,031 + EX28DRAFT_3729 0.83 -1.7 405,068 - EX28DRAFT_3729 0.87 +0.7 405,110 - -0.1 405,110 - -3.7 405,116 - +0.1 405,243 + -1.8 405,243 + -1.1 405,244 - +0.1 405,245 + -0.7 405,246 - -0.6 405,246 - -1.7 405,414 + EX28DRAFT_3730 0.28 +0.1 405,531 + EX28DRAFT_3730 0.43 -0.0 405,531 + EX28DRAFT_3730 0.43 -2.4 405,531 + EX28DRAFT_3730 0.43 +0.4 405,531 + EX28DRAFT_3730 0.43 +0.5 405,531 + EX28DRAFT_3730 0.43 -2.4 405,534 - EX28DRAFT_3730 0.43 -2.2 405,778 - EX28DRAFT_3730 0.74 -1.6 405,902 + -2.0 405,903 - -0.7 405,909 - -1.3 405,959 - -0.7 405,981 + -0.3 406,029 - +0.5 406,052 + +0.2 406,052 + +0.1 406,052 + +0.8 406,053 - +0.1 406,053 - +0.4 406,056 + -0.1 406,955 - EX28DRAFT_3732 0.27 +0.8 406,955 - EX28DRAFT_3732 0.27 -3.6 406,955 - EX28DRAFT_3732 0.27 +0.1
Or see this region's nucleotide sequence