Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3668 and EX28DRAFT_3669 are separated by 2 nucleotides EX28DRAFT_3669 and EX28DRAFT_3670 overlap by 1 nucleotides
EX28DRAFT_3668: EX28DRAFT_3668 - 3-isopropylmalate dehydratase, large subunit, at 334,392 to 335,792
_3668
EX28DRAFT_3669: EX28DRAFT_3669 - 3-isopropylmalate dehydrogenase, at 335,795 to 336,886
_3669
EX28DRAFT_3670: EX28DRAFT_3670 - 2-isopropylmalate synthase (EC 2.3.3.13), at 336,886 to 338,457
_3670
Position (kb) 335
336
337 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 335.068 kb on + strand, within EX28DRAFT_3668 at 335.068 kb on + strand, within EX28DRAFT_3668 at 335.130 kb on - strand, within EX28DRAFT_3668 at 335.501 kb on - strand, within EX28DRAFT_3668 at 335.716 kb on + strand at 335.747 kb on - strand at 335.776 kb on + strand at 335.776 kb on + strand at 335.776 kb on + strand at 335.778 kb on + strand at 335.779 kb on - strand at 335.787 kb on - strand at 335.796 kb on + strand at 335.796 kb on + strand at 335.797 kb on - strand at 335.815 kb on - strand at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.213 kb on + strand, within EX28DRAFT_3669 at 336.214 kb on - strand, within EX28DRAFT_3669 at 336.323 kb on - strand, within EX28DRAFT_3669 at 336.323 kb on - strand, within EX28DRAFT_3669 at 336.388 kb on - strand, within EX28DRAFT_3669 at 336.388 kb on - strand, within EX28DRAFT_3669 at 336.388 kb on - strand, within EX28DRAFT_3669 at 336.760 kb on - strand, within EX28DRAFT_3669 at 336.869 kb on - strand at 336.874 kb on - strand at 337.112 kb on + strand, within EX28DRAFT_3670 at 337.160 kb on + strand, within EX28DRAFT_3670 at 337.161 kb on - strand, within EX28DRAFT_3670 at 337.161 kb on - strand, within EX28DRAFT_3670 at 337.274 kb on + strand, within EX28DRAFT_3670 at 337.315 kb on - strand, within EX28DRAFT_3670 at 337.419 kb on + strand, within EX28DRAFT_3670 at 337.419 kb on + strand, within EX28DRAFT_3670 at 337.421 kb on + strand, within EX28DRAFT_3670 at 337.421 kb on + strand, within EX28DRAFT_3670 at 337.421 kb on + strand, within EX28DRAFT_3670 at 337.421 kb on + strand, within EX28DRAFT_3670 at 337.438 kb on - strand, within EX28DRAFT_3670 at 337.486 kb on + strand, within EX28DRAFT_3670 at 337.486 kb on + strand, within EX28DRAFT_3670 at 337.695 kb on + strand, within EX28DRAFT_3670 at 337.762 kb on + strand, within EX28DRAFT_3670 at 337.765 kb on - strand, within EX28DRAFT_3670 at 337.821 kb on + strand, within EX28DRAFT_3670 at 337.864 kb on - strand, within EX28DRAFT_3670
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 334,819 + EX28DRAFT_3668 0.30 -4.9 334,819 + EX28DRAFT_3668 0.30 -3.4 334,819 + EX28DRAFT_3668 0.30 -3.3 334,819 + EX28DRAFT_3668 0.30 -2.6 334,819 + EX28DRAFT_3668 0.30 -6.0 334,819 + EX28DRAFT_3668 0.30 -2.6 334,819 + EX28DRAFT_3668 0.30 -4.1 335,068 + EX28DRAFT_3668 0.48 -4.4 335,068 + EX28DRAFT_3668 0.48 -5.0 335,130 - EX28DRAFT_3668 0.53 -3.8 335,501 - EX28DRAFT_3668 0.79 -4.4 335,716 + -3.4 335,747 - -3.7 335,776 + -0.9 335,776 + -3.9 335,776 + -2.7 335,778 + -7.6 335,779 - +0.1 335,787 - -2.0 335,796 + -1.1 335,796 + -3.9 335,797 - -6.5 335,815 - -4.8 336,213 + EX28DRAFT_3669 0.38 -2.4 336,213 + EX28DRAFT_3669 0.38 -4.0 336,213 + EX28DRAFT_3669 0.38 -5.1 336,213 + EX28DRAFT_3669 0.38 -5.7 336,213 + EX28DRAFT_3669 0.38 -3.2 336,214 - EX28DRAFT_3669 0.38 -5.7 336,323 - EX28DRAFT_3669 0.48 -5.1 336,323 - EX28DRAFT_3669 0.48 -3.6 336,388 - EX28DRAFT_3669 0.54 -3.1 336,388 - EX28DRAFT_3669 0.54 +0.1 336,388 - EX28DRAFT_3669 0.54 -3.6 336,760 - EX28DRAFT_3669 0.88 -4.4 336,869 - -5.8 336,874 - -4.8 337,112 + EX28DRAFT_3670 0.14 -5.7 337,160 + EX28DRAFT_3670 0.17 -4.3 337,161 - EX28DRAFT_3670 0.17 -0.5 337,161 - EX28DRAFT_3670 0.17 -0.5 337,274 + EX28DRAFT_3670 0.25 -2.2 337,315 - EX28DRAFT_3670 0.27 -1.0 337,419 + EX28DRAFT_3670 0.34 -3.2 337,419 + EX28DRAFT_3670 0.34 -3.2 337,421 + EX28DRAFT_3670 0.34 -5.0 337,421 + EX28DRAFT_3670 0.34 -2.7 337,421 + EX28DRAFT_3670 0.34 -5.6 337,421 + EX28DRAFT_3670 0.34 -4.3 337,438 - EX28DRAFT_3670 0.35 -5.9 337,486 + EX28DRAFT_3670 0.38 -2.8 337,486 + EX28DRAFT_3670 0.38 -4.0 337,695 + EX28DRAFT_3670 0.51 -3.8 337,762 + EX28DRAFT_3670 0.56 -3.0 337,765 - EX28DRAFT_3670 0.56 -6.9 337,821 + EX28DRAFT_3670 0.59 -5.1 337,864 - EX28DRAFT_3670 0.62 -5.1
Or see this region's nucleotide sequence