Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3666 and EX28DRAFT_3667 are separated by 162 nucleotides EX28DRAFT_3667 and EX28DRAFT_3668 are separated by 10 nucleotides
EX28DRAFT_3666: EX28DRAFT_3666 - cation diffusion facilitator family transporter, at 332,723 to 333,613
_3666
EX28DRAFT_3667: EX28DRAFT_3667 - 3-isopropylmalate dehydratase, small subunit, at 333,776 to 334,381
_3667
EX28DRAFT_3668: EX28DRAFT_3668 - 3-isopropylmalate dehydratase, large subunit, at 334,392 to 335,792
_3668
Position (kb) 333
334
335 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 332.783 kb on - strand at 332.825 kb on + strand, within EX28DRAFT_3666 at 332.826 kb on - strand, within EX28DRAFT_3666 at 333.027 kb on - strand, within EX28DRAFT_3666 at 333.167 kb on - strand, within EX28DRAFT_3666 at 333.189 kb on + strand, within EX28DRAFT_3666 at 333.190 kb on - strand, within EX28DRAFT_3666 at 333.190 kb on - strand, within EX28DRAFT_3666 at 333.284 kb on - strand, within EX28DRAFT_3666 at 333.284 kb on - strand, within EX28DRAFT_3666 at 333.338 kb on - strand, within EX28DRAFT_3666 at 333.413 kb on - strand, within EX28DRAFT_3666 at 333.444 kb on - strand, within EX28DRAFT_3666 at 333.498 kb on - strand, within EX28DRAFT_3666 at 333.536 kb on + strand at 333.558 kb on + strand at 333.558 kb on + strand at 333.558 kb on + strand at 333.559 kb on - strand at 333.563 kb on - strand at 333.566 kb on + strand at 333.675 kb on + strand at 333.675 kb on + strand at 333.710 kb on + strand at 333.711 kb on - strand at 333.714 kb on + strand at 333.715 kb on - strand at 333.903 kb on + strand, within EX28DRAFT_3667 at 334.095 kb on + strand, within EX28DRAFT_3667 at 334.306 kb on + strand, within EX28DRAFT_3667 at 334.307 kb on - strand, within EX28DRAFT_3667 at 334.309 kb on + strand, within EX28DRAFT_3667 at 334.357 kb on + strand at 334.357 kb on + strand at 334.357 kb on + strand at 334.393 kb on + strand at 334.394 kb on - strand at 334.503 kb on - strand at 334.666 kb on - strand, within EX28DRAFT_3668 at 334.666 kb on - strand, within EX28DRAFT_3668 at 334.772 kb on + strand, within EX28DRAFT_3668 at 334.772 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 334.819 kb on + strand, within EX28DRAFT_3668 at 335.068 kb on + strand, within EX28DRAFT_3668 at 335.068 kb on + strand, within EX28DRAFT_3668 at 335.130 kb on - strand, within EX28DRAFT_3668
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 332,783 - -1.2 332,825 + EX28DRAFT_3666 0.11 -1.0 332,826 - EX28DRAFT_3666 0.12 -1.8 333,027 - EX28DRAFT_3666 0.34 +0.1 333,167 - EX28DRAFT_3666 0.50 -0.9 333,189 + EX28DRAFT_3666 0.52 -1.1 333,190 - EX28DRAFT_3666 0.52 +0.4 333,190 - EX28DRAFT_3666 0.52 +1.0 333,284 - EX28DRAFT_3666 0.63 -0.4 333,284 - EX28DRAFT_3666 0.63 -0.7 333,338 - EX28DRAFT_3666 0.69 +0.1 333,413 - EX28DRAFT_3666 0.77 +0.4 333,444 - EX28DRAFT_3666 0.81 +0.4 333,498 - EX28DRAFT_3666 0.87 -0.1 333,536 + +0.6 333,558 + +0.6 333,558 + +0.1 333,558 + -1.4 333,559 - +2.9 333,563 - +0.8 333,566 + +0.8 333,675 + +0.6 333,675 + +0.1 333,710 + +0.8 333,711 - +1.5 333,714 + +1.7 333,715 - -1.8 333,903 + EX28DRAFT_3667 0.21 -6.0 334,095 + EX28DRAFT_3667 0.53 -4.8 334,306 + EX28DRAFT_3667 0.87 -3.6 334,307 - EX28DRAFT_3667 0.88 -4.3 334,309 + EX28DRAFT_3667 0.88 -2.7 334,357 + -6.1 334,357 + -5.8 334,357 + -6.0 334,393 + -6.0 334,394 - -1.1 334,503 - -5.8 334,666 - EX28DRAFT_3668 0.20 -2.1 334,666 - EX28DRAFT_3668 0.20 -0.9 334,772 + EX28DRAFT_3668 0.27 -4.0 334,772 + EX28DRAFT_3668 0.27 -4.5 334,819 + EX28DRAFT_3668 0.30 -4.9 334,819 + EX28DRAFT_3668 0.30 -3.4 334,819 + EX28DRAFT_3668 0.30 -3.3 334,819 + EX28DRAFT_3668 0.30 -2.6 334,819 + EX28DRAFT_3668 0.30 -6.0 334,819 + EX28DRAFT_3668 0.30 -2.6 334,819 + EX28DRAFT_3668 0.30 -4.1 335,068 + EX28DRAFT_3668 0.48 -4.4 335,068 + EX28DRAFT_3668 0.48 -5.0 335,130 - EX28DRAFT_3668 0.53 -3.8
Or see this region's nucleotide sequence