Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3573 and EX28DRAFT_3574 are separated by 254 nucleotides EX28DRAFT_3574 and EX28DRAFT_3575 are separated by 41 nucleotides EX28DRAFT_3575 and EX28DRAFT_3576 are separated by 33 nucleotides EX28DRAFT_3576 and EX28DRAFT_3577 are separated by 33 nucleotides EX28DRAFT_3577 and EX28DRAFT_3578 are separated by 28 nucleotides EX28DRAFT_3578 and EX28DRAFT_3579 are separated by 172 nucleotides
EX28DRAFT_3573: EX28DRAFT_3573 - diguanylate cyclase (GGDEF) domain, at 234,964 to 236,028
_3573
EX28DRAFT_3574: EX28DRAFT_3574 - Protein of unknown function (DUF1435), at 236,283 to 236,513
_3574
EX28DRAFT_3575: EX28DRAFT_3575 - tRNA, at 236,555 to 236,640
_3575
EX28DRAFT_3576: EX28DRAFT_3576 - tRNA, at 236,674 to 236,759
_3576
EX28DRAFT_3577: EX28DRAFT_3577 - tRNA, at 236,793 to 236,879
_3577
EX28DRAFT_3578: EX28DRAFT_3578 - tRNA, at 236,908 to 236,994
_3578
EX28DRAFT_3579: EX28DRAFT_3579 - 16S RNA G1207 methylase RsmC, at 237,167 to 238,195
_3579
Position (kb)
236
237 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 235.317 kb on + strand, within EX28DRAFT_3573 at 235.318 kb on - strand, within EX28DRAFT_3573 at 235.318 kb on - strand, within EX28DRAFT_3573 at 235.337 kb on + strand, within EX28DRAFT_3573 at 235.337 kb on + strand, within EX28DRAFT_3573 at 235.338 kb on - strand, within EX28DRAFT_3573 at 235.338 kb on - strand, within EX28DRAFT_3573 at 235.434 kb on + strand, within EX28DRAFT_3573 at 235.436 kb on + strand, within EX28DRAFT_3573 at 235.437 kb on - strand, within EX28DRAFT_3573 at 235.586 kb on + strand, within EX28DRAFT_3573 at 235.604 kb on - strand, within EX28DRAFT_3573 at 235.605 kb on + strand, within EX28DRAFT_3573 at 235.605 kb on + strand, within EX28DRAFT_3573 at 235.660 kb on - strand, within EX28DRAFT_3573 at 235.660 kb on - strand, within EX28DRAFT_3573 at 235.730 kb on + strand, within EX28DRAFT_3573 at 235.730 kb on + strand, within EX28DRAFT_3573 at 235.818 kb on + strand, within EX28DRAFT_3573 at 235.818 kb on + strand, within EX28DRAFT_3573 at 235.819 kb on - strand, within EX28DRAFT_3573 at 235.888 kb on - strand, within EX28DRAFT_3573 at 235.888 kb on - strand, within EX28DRAFT_3573 at 235.888 kb on - strand, within EX28DRAFT_3573 at 235.901 kb on - strand, within EX28DRAFT_3573 at 235.964 kb on - strand at 236.065 kb on + strand at 236.467 kb on + strand, within EX28DRAFT_3574 at 236.546 kb on + strand at 236.547 kb on - strand at 236.552 kb on - strand at 236.552 kb on - strand at 236.552 kb on - strand at 236.599 kb on - strand, within EX28DRAFT_3575 at 236.851 kb on - strand, within EX28DRAFT_3577 at 236.894 kb on - strand at 236.894 kb on - strand at 236.907 kb on - strand at 236.907 kb on - strand at 237.017 kb on - strand at 237.168 kb on + strand at 237.316 kb on + strand, within EX28DRAFT_3579 at 237.400 kb on + strand, within EX28DRAFT_3579 at 237.401 kb on - strand, within EX28DRAFT_3579 at 237.497 kb on + strand, within EX28DRAFT_3579
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 235,317 + EX28DRAFT_3573 0.33 -0.4 235,318 - EX28DRAFT_3573 0.33 -0.5 235,318 - EX28DRAFT_3573 0.33 -0.0 235,337 + EX28DRAFT_3573 0.35 +0.3 235,337 + EX28DRAFT_3573 0.35 -0.5 235,338 - EX28DRAFT_3573 0.35 -0.5 235,338 - EX28DRAFT_3573 0.35 +1.5 235,434 + EX28DRAFT_3573 0.44 -0.8 235,436 + EX28DRAFT_3573 0.44 +0.3 235,437 - EX28DRAFT_3573 0.44 -0.4 235,586 + EX28DRAFT_3573 0.58 -3.0 235,604 - EX28DRAFT_3573 0.60 -0.7 235,605 + EX28DRAFT_3573 0.60 -0.4 235,605 + EX28DRAFT_3573 0.60 -0.8 235,660 - EX28DRAFT_3573 0.65 +0.9 235,660 - EX28DRAFT_3573 0.65 +0.1 235,730 + EX28DRAFT_3573 0.72 -0.9 235,730 + EX28DRAFT_3573 0.72 -0.8 235,818 + EX28DRAFT_3573 0.80 +0.3 235,818 + EX28DRAFT_3573 0.80 -0.7 235,819 - EX28DRAFT_3573 0.80 -0.9 235,888 - EX28DRAFT_3573 0.87 +0.5 235,888 - EX28DRAFT_3573 0.87 +0.4 235,888 - EX28DRAFT_3573 0.87 +0.1 235,901 - EX28DRAFT_3573 0.88 +0.4 235,964 - -0.2 236,065 + +0.3 236,467 + EX28DRAFT_3574 0.80 -1.1 236,546 + -1.1 236,547 - -0.6 236,552 - -1.6 236,552 - +0.4 236,552 - -0.2 236,599 - EX28DRAFT_3575 0.51 +0.6 236,851 - EX28DRAFT_3577 0.67 -0.2 236,894 - -4.1 236,894 - +0.9 236,907 - +0.2 236,907 - +0.1 237,017 - +0.0 237,168 + -0.3 237,316 + EX28DRAFT_3579 0.14 +0.8 237,400 + EX28DRAFT_3579 0.23 -1.3 237,401 - EX28DRAFT_3579 0.23 -2.4 237,497 + EX28DRAFT_3579 0.32 +0.4
Or see this region's nucleotide sequence