Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3221 and EX28DRAFT_3222 are separated by 17 nucleotides EX28DRAFT_3222 and EX28DRAFT_3223 are separated by 89 nucleotides EX28DRAFT_3223 and EX28DRAFT_3224 are separated by 287 nucleotides EX28DRAFT_3224 and EX28DRAFT_3225 are separated by 191 nucleotides EX28DRAFT_3225 and EX28DRAFT_3226 are separated by 9 nucleotides EX28DRAFT_3226 and EX28DRAFT_3227 are separated by 223 nucleotides
EX28DRAFT_3221: EX28DRAFT_3221 - Uncharacterized protein conserved in bacteria, at 521,432 to 521,884
_3221
EX28DRAFT_3222: EX28DRAFT_3222 - RNA, at 521,902 to 522,002
_3222
EX28DRAFT_3223: EX28DRAFT_3223 - tRNA, at 522,092 to 522,168
_3223
EX28DRAFT_3224: EX28DRAFT_3224 - argininosuccinate synthase (EC 6.3.4.5), at 522,456 to 523,802
_3224
EX28DRAFT_3225: EX28DRAFT_3225 - tRNA, at 523,994 to 524,080
_3225
EX28DRAFT_3226: EX28DRAFT_3226 - protein translocase, SecG subunit, at 524,090 to 524,422
_3226
EX28DRAFT_3227: EX28DRAFT_3227 - phosphoglucosamine mutase (EC 5.4.2.10), at 524,646 to 525,983
_3227
Position (kb)
522
523
524 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 522.214 kb on + strand at 522.214 kb on + strand at 522.259 kb on + strand at 522.263 kb on - strand at 522.265 kb on - strand at 522.270 kb on + strand at 522.312 kb on - strand at 522.455 kb on + strand at 522.494 kb on + strand at 522.495 kb on - strand at 522.576 kb on + strand at 522.613 kb on - strand, within EX28DRAFT_3224 at 522.613 kb on - strand, within EX28DRAFT_3224 at 522.643 kb on - strand, within EX28DRAFT_3224 at 522.643 kb on - strand, within EX28DRAFT_3224 at 522.751 kb on - strand, within EX28DRAFT_3224 at 522.852 kb on - strand, within EX28DRAFT_3224 at 522.916 kb on - strand, within EX28DRAFT_3224 at 523.005 kb on - strand, within EX28DRAFT_3224 at 523.027 kb on - strand, within EX28DRAFT_3224 at 523.400 kb on + strand, within EX28DRAFT_3224 at 523.401 kb on - strand, within EX28DRAFT_3224 at 523.401 kb on - strand, within EX28DRAFT_3224 at 523.534 kb on + strand, within EX28DRAFT_3224 at 523.563 kb on + strand, within EX28DRAFT_3224 at 523.600 kb on - strand, within EX28DRAFT_3224 at 523.679 kb on + strand at 523.679 kb on + strand at 523.719 kb on + strand at 523.719 kb on + strand at 523.719 kb on + strand at 523.720 kb on - strand at 523.721 kb on + strand at 523.801 kb on - strand at 523.810 kb on + strand at 523.810 kb on + strand at 523.813 kb on - strand at 523.813 kb on - strand at 523.813 kb on - strand at 523.846 kb on + strand at 523.948 kb on - strand at 523.948 kb on - strand at 524.423 kb on - strand at 524.557 kb on - strand at 524.634 kb on + strand at 524.635 kb on - strand at 524.647 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 522,214 + -0.3 522,214 + -0.9 522,259 + +0.5 522,263 - +1.5 522,265 - -0.6 522,270 + +0.5 522,312 - -0.3 522,455 + -5.1 522,494 + -6.2 522,495 - -6.6 522,576 + -5.8 522,613 - EX28DRAFT_3224 0.12 -4.6 522,613 - EX28DRAFT_3224 0.12 -2.7 522,643 - EX28DRAFT_3224 0.14 -5.2 522,643 - EX28DRAFT_3224 0.14 -3.3 522,751 - EX28DRAFT_3224 0.22 -5.5 522,852 - EX28DRAFT_3224 0.29 -4.1 522,916 - EX28DRAFT_3224 0.34 -4.8 523,005 - EX28DRAFT_3224 0.41 -3.2 523,027 - EX28DRAFT_3224 0.42 -2.7 523,400 + EX28DRAFT_3224 0.70 -3.5 523,401 - EX28DRAFT_3224 0.70 -5.9 523,401 - EX28DRAFT_3224 0.70 -4.5 523,534 + EX28DRAFT_3224 0.80 -2.5 523,563 + EX28DRAFT_3224 0.82 -3.0 523,600 - EX28DRAFT_3224 0.85 -3.3 523,679 + -3.1 523,679 + -4.0 523,719 + -3.2 523,719 + -5.7 523,719 + -3.7 523,720 - -5.3 523,721 + -1.9 523,801 - -0.5 523,810 + -0.5 523,810 + +0.5 523,813 - -0.1 523,813 - -0.7 523,813 - -0.6 523,846 + -0.8 523,948 - -0.0 523,948 - -0.5 524,423 - -0.5 524,557 - +0.3 524,634 + -1.2 524,635 - +0.0 524,647 + -0.3
Or see this region's nucleotide sequence