Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_3015 and EX28DRAFT_3016 are separated by 94 nucleotides EX28DRAFT_3016 and EX28DRAFT_3017 are separated by 170 nucleotides EX28DRAFT_3017 and EX28DRAFT_3018 are separated by 622 nucleotides
EX28DRAFT_3015: EX28DRAFT_3015 - hypothetical protein, at 323,994 to 324,386
_3015
EX28DRAFT_3016: EX28DRAFT_3016 - Protein of unknown function (DUF1493), at 324,481 to 324,861
_3016
EX28DRAFT_3017: EX28DRAFT_3017 - Bacterial regulatory proteins, luxR family, at 325,032 to 325,610
_3017
EX28DRAFT_3018: EX28DRAFT_3018 - poly-beta-1,6 N-acetyl-D-glucosamine export porin PgaA, at 326,233 to 328,671
_3018
Position (kb) 325
326 Strain fitness (log2 ratio) -2
-1
0
1 at 324.089 kb on + strand, within EX28DRAFT_3015 at 324.089 kb on + strand, within EX28DRAFT_3015 at 324.174 kb on - strand, within EX28DRAFT_3015 at 324.174 kb on - strand, within EX28DRAFT_3015 at 324.174 kb on - strand, within EX28DRAFT_3015 at 324.182 kb on + strand, within EX28DRAFT_3015 at 324.259 kb on + strand, within EX28DRAFT_3015 at 324.259 kb on + strand, within EX28DRAFT_3015 at 324.376 kb on + strand at 324.377 kb on - strand at 324.381 kb on - strand at 324.402 kb on - strand at 324.438 kb on - strand at 324.454 kb on - strand at 324.509 kb on - strand at 324.526 kb on - strand, within EX28DRAFT_3016 at 324.528 kb on - strand, within EX28DRAFT_3016 at 324.528 kb on - strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.585 kb on + strand, within EX28DRAFT_3016 at 324.586 kb on - strand, within EX28DRAFT_3016 at 324.586 kb on - strand, within EX28DRAFT_3016 at 324.612 kb on - strand, within EX28DRAFT_3016 at 324.674 kb on + strand, within EX28DRAFT_3016 at 324.674 kb on + strand, within EX28DRAFT_3016 at 324.755 kb on - strand, within EX28DRAFT_3016 at 324.805 kb on - strand, within EX28DRAFT_3016 at 324.836 kb on - strand at 324.857 kb on - strand at 324.857 kb on - strand at 324.901 kb on - strand at 324.901 kb on - strand at 324.941 kb on - strand at 324.990 kb on - strand at 325.009 kb on + strand at 325.009 kb on + strand at 325.009 kb on + strand at 325.010 kb on - strand at 325.010 kb on - strand at 325.236 kb on - strand, within EX28DRAFT_3017 at 325.248 kb on - strand, within EX28DRAFT_3017 at 325.303 kb on - strand, within EX28DRAFT_3017 at 325.344 kb on + strand, within EX28DRAFT_3017 at 325.344 kb on + strand, within EX28DRAFT_3017 at 325.345 kb on - strand, within EX28DRAFT_3017 at 325.352 kb on + strand, within EX28DRAFT_3017 at 325.438 kb on + strand, within EX28DRAFT_3017 at 325.438 kb on + strand, within EX28DRAFT_3017 at 325.445 kb on - strand, within EX28DRAFT_3017 at 325.446 kb on + strand, within EX28DRAFT_3017 at 325.506 kb on + strand, within EX28DRAFT_3017 at 325.506 kb on + strand, within EX28DRAFT_3017 at 326.488 kb on - strand, within EX28DRAFT_3018 at 326.515 kb on + strand, within EX28DRAFT_3018 at 326.515 kb on + strand, within EX28DRAFT_3018 at 326.518 kb on - strand, within EX28DRAFT_3018
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 324,089 + EX28DRAFT_3015 0.24 -0.6 324,089 + EX28DRAFT_3015 0.24 +0.3 324,174 - EX28DRAFT_3015 0.46 -1.2 324,174 - EX28DRAFT_3015 0.46 +0.4 324,174 - EX28DRAFT_3015 0.46 +0.8 324,182 + EX28DRAFT_3015 0.48 -0.3 324,259 + EX28DRAFT_3015 0.67 +0.6 324,259 + EX28DRAFT_3015 0.67 -0.9 324,376 + -1.5 324,377 - +0.2 324,381 - +0.0 324,402 - +0.2 324,438 - +0.4 324,454 - +0.1 324,509 - +0.5 324,526 - EX28DRAFT_3016 0.12 +0.2 324,528 - EX28DRAFT_3016 0.12 +0.9 324,528 - EX28DRAFT_3016 0.12 -0.1 324,585 + EX28DRAFT_3016 0.27 -0.5 324,585 + EX28DRAFT_3016 0.27 -1.6 324,585 + EX28DRAFT_3016 0.27 -0.3 324,585 + EX28DRAFT_3016 0.27 -0.4 324,585 + EX28DRAFT_3016 0.27 +1.3 324,586 - EX28DRAFT_3016 0.28 +0.1 324,586 - EX28DRAFT_3016 0.28 -0.0 324,612 - EX28DRAFT_3016 0.34 +0.8 324,674 + EX28DRAFT_3016 0.51 -1.7 324,674 + EX28DRAFT_3016 0.51 -0.3 324,755 - EX28DRAFT_3016 0.72 -1.9 324,805 - EX28DRAFT_3016 0.85 +0.8 324,836 - +0.6 324,857 - +1.3 324,857 - +0.2 324,901 - +0.4 324,901 - -1.1 324,941 - +0.1 324,990 - -1.9 325,009 + -0.8 325,009 + -1.3 325,009 + +1.6 325,010 - -0.8 325,010 - -1.9 325,236 - EX28DRAFT_3017 0.35 -0.5 325,248 - EX28DRAFT_3017 0.37 +0.4 325,303 - EX28DRAFT_3017 0.47 +0.1 325,344 + EX28DRAFT_3017 0.54 +0.0 325,344 + EX28DRAFT_3017 0.54 -0.4 325,345 - EX28DRAFT_3017 0.54 -0.9 325,352 + EX28DRAFT_3017 0.55 -0.5 325,438 + EX28DRAFT_3017 0.70 -0.3 325,438 + EX28DRAFT_3017 0.70 +0.7 325,445 - EX28DRAFT_3017 0.71 -0.9 325,446 + EX28DRAFT_3017 0.72 +0.2 325,506 + EX28DRAFT_3017 0.82 +0.1 325,506 + EX28DRAFT_3017 0.82 -0.7 326,488 - EX28DRAFT_3018 0.10 +0.0 326,515 + EX28DRAFT_3018 0.12 +0.0 326,515 + EX28DRAFT_3018 0.12 +1.4 326,518 - EX28DRAFT_3018 0.12 -0.1
Or see this region's nucleotide sequence