Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2883 and EX28DRAFT_2884 are separated by 128 nucleotides EX28DRAFT_2884 and EX28DRAFT_2885 are separated by 13 nucleotides EX28DRAFT_2885 and EX28DRAFT_2886 are separated by 192 nucleotides EX28DRAFT_2886 and EX28DRAFT_2887 overlap by 1 nucleotides EX28DRAFT_2887 and EX28DRAFT_2888 overlap by 10 nucleotides
EX28DRAFT_2883: EX28DRAFT_2883 - Transcriptional regulator, at 189,786 to 190,703
_2883
EX28DRAFT_2884: EX28DRAFT_2884 - RNA, at 190,832 to 191,039
_2884
EX28DRAFT_2885: EX28DRAFT_2885 - Bacterial protein of unknown function (DUF903), at 191,053 to 191,280
_2885
EX28DRAFT_2886: EX28DRAFT_2886 - cysteine desulfurase, catalytic subunit CsdA, at 191,473 to 192,678
_2886
EX28DRAFT_2887: EX28DRAFT_2887 - cysteine desulfurase, sulfur acceptor subunit CsdE, at 192,678 to 193,124
_2887
EX28DRAFT_2888: EX28DRAFT_2888 - Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 1, at 193,115 to 193,921
_2888
Position (kb) 191
192
193 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 190.635 kb on - strand at 190.806 kb on + strand at 190.806 kb on + strand at 190.816 kb on + strand at 190.820 kb on + strand at 190.820 kb on + strand at 190.820 kb on + strand at 190.820 kb on + strand at 190.820 kb on + strand at 190.821 kb on - strand at 190.822 kb on + strand at 190.983 kb on + strand, within EX28DRAFT_2884 at 190.983 kb on + strand, within EX28DRAFT_2884 at 190.984 kb on - strand, within EX28DRAFT_2884 at 191.005 kb on + strand, within EX28DRAFT_2884 at 191.010 kb on + strand, within EX28DRAFT_2884 at 191.011 kb on - strand, within EX28DRAFT_2884 at 191.179 kb on - strand, within EX28DRAFT_2885 at 191.207 kb on + strand, within EX28DRAFT_2885 at 191.207 kb on + strand, within EX28DRAFT_2885 at 191.207 kb on + strand, within EX28DRAFT_2885 at 191.208 kb on - strand, within EX28DRAFT_2885 at 191.208 kb on - strand, within EX28DRAFT_2885 at 191.275 kb on - strand at 191.285 kb on - strand at 191.357 kb on - strand at 191.382 kb on + strand at 191.409 kb on + strand at 191.409 kb on + strand at 191.410 kb on - strand at 191.452 kb on + strand at 191.453 kb on - strand at 191.453 kb on - strand at 191.454 kb on + strand at 191.537 kb on - strand at 191.537 kb on - strand at 191.600 kb on - strand, within EX28DRAFT_2886 at 191.757 kb on + strand, within EX28DRAFT_2886 at 191.757 kb on + strand, within EX28DRAFT_2886 at 192.119 kb on - strand, within EX28DRAFT_2886 at 192.135 kb on + strand, within EX28DRAFT_2886 at 192.135 kb on + strand, within EX28DRAFT_2886 at 192.135 kb on + strand, within EX28DRAFT_2886 at 192.136 kb on - strand, within EX28DRAFT_2886 at 192.136 kb on - strand, within EX28DRAFT_2886 at 192.610 kb on - strand at 192.612 kb on + strand at 192.665 kb on + strand at 192.666 kb on - strand at 192.684 kb on - strand at 192.737 kb on - strand, within EX28DRAFT_2887 at 192.773 kb on + strand, within EX28DRAFT_2887 at 192.774 kb on - strand, within EX28DRAFT_2887 at 192.777 kb on + strand, within EX28DRAFT_2887 at 192.850 kb on + strand, within EX28DRAFT_2887 at 192.851 kb on - strand, within EX28DRAFT_2887 at 192.851 kb on - strand, within EX28DRAFT_2887 at 193.551 kb on + strand, within EX28DRAFT_2888 at 193.551 kb on + strand, within EX28DRAFT_2888 at 193.552 kb on - strand, within EX28DRAFT_2888 at 193.557 kb on + strand, within EX28DRAFT_2888 at 193.558 kb on - strand, within EX28DRAFT_2888 at 193.558 kb on - strand, within EX28DRAFT_2888 at 193.574 kb on - strand, within EX28DRAFT_2888
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 190,635 - -0.9 190,806 + +0.0 190,806 + +2.7 190,816 + -0.4 190,820 + +0.9 190,820 + +0.7 190,820 + -0.2 190,820 + +0.3 190,820 + +1.0 190,821 - +0.6 190,822 + -1.5 190,983 + EX28DRAFT_2884 0.73 +0.5 190,983 + EX28DRAFT_2884 0.73 +0.6 190,984 - EX28DRAFT_2884 0.73 +3.7 191,005 + EX28DRAFT_2884 0.83 +0.3 191,010 + EX28DRAFT_2884 0.86 -0.1 191,011 - EX28DRAFT_2884 0.86 +0.6 191,179 - EX28DRAFT_2885 0.55 -0.5 191,207 + EX28DRAFT_2885 0.68 -0.7 191,207 + EX28DRAFT_2885 0.68 -2.0 191,207 + EX28DRAFT_2885 0.68 +0.2 191,208 - EX28DRAFT_2885 0.68 +1.1 191,208 - EX28DRAFT_2885 0.68 -1.4 191,275 - +0.6 191,285 - +1.5 191,357 - +0.7 191,382 + +0.7 191,409 + +0.2 191,409 + -0.3 191,410 - +0.0 191,452 + +1.8 191,453 - -0.4 191,453 - -1.8 191,454 + -1.0 191,537 - -0.4 191,537 - -0.5 191,600 - EX28DRAFT_2886 0.11 +0.7 191,757 + EX28DRAFT_2886 0.24 +0.2 191,757 + EX28DRAFT_2886 0.24 +0.5 192,119 - EX28DRAFT_2886 0.54 +0.1 192,135 + EX28DRAFT_2886 0.55 -3.1 192,135 + EX28DRAFT_2886 0.55 -0.2 192,135 + EX28DRAFT_2886 0.55 +0.4 192,136 - EX28DRAFT_2886 0.55 +0.5 192,136 - EX28DRAFT_2886 0.55 +0.0 192,610 - -0.2 192,612 + +0.1 192,665 + -0.0 192,666 - -0.3 192,684 - -0.2 192,737 - EX28DRAFT_2887 0.13 -0.8 192,773 + EX28DRAFT_2887 0.21 -1.0 192,774 - EX28DRAFT_2887 0.21 -2.2 192,777 + EX28DRAFT_2887 0.22 +1.0 192,850 + EX28DRAFT_2887 0.38 -0.1 192,851 - EX28DRAFT_2887 0.39 +0.5 192,851 - EX28DRAFT_2887 0.39 -0.4 193,551 + EX28DRAFT_2888 0.54 +1.6 193,551 + EX28DRAFT_2888 0.54 +0.8 193,552 - EX28DRAFT_2888 0.54 +0.4 193,557 + EX28DRAFT_2888 0.55 +1.0 193,558 - EX28DRAFT_2888 0.55 +0.2 193,558 - EX28DRAFT_2888 0.55 -1.4 193,574 - EX28DRAFT_2888 0.57 -0.0
Or see this region's nucleotide sequence