Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2852 and EX28DRAFT_2853 overlap by 1 nucleotides EX28DRAFT_2853 and EX28DRAFT_2854 are separated by 306 nucleotides EX28DRAFT_2854 and EX28DRAFT_2855 are separated by 85 nucleotides
EX28DRAFT_2852: EX28DRAFT_2852 - sulfite reductase (NADPH) hemoprotein, beta-component, at 152,958 to 154,670
_2852
EX28DRAFT_2853: EX28DRAFT_2853 - sulfite reductase [NADPH] flavoprotein, alpha-component, at 154,670 to 156,475
_2853
EX28DRAFT_2854: EX28DRAFT_2854 - queuosine biosynthesis protein QueD, at 156,782 to 157,144
_2854
EX28DRAFT_2855: EX28DRAFT_2855 - putative 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE, at 157,230 to 157,901
_2855
Position (kb) 154
155
156
157 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 153.745 kb on + strand, within EX28DRAFT_2852 at 153.746 kb on - strand, within EX28DRAFT_2852 at 153.746 kb on - strand, within EX28DRAFT_2852 at 153.746 kb on - strand, within EX28DRAFT_2852 at 153.746 kb on - strand, within EX28DRAFT_2852 at 153.746 kb on - strand, within EX28DRAFT_2852 at 153.872 kb on - strand, within EX28DRAFT_2852 at 153.923 kb on + strand, within EX28DRAFT_2852 at 154.039 kb on + strand, within EX28DRAFT_2852 at 154.040 kb on - strand, within EX28DRAFT_2852 at 154.159 kb on + strand, within EX28DRAFT_2852 at 154.345 kb on + strand, within EX28DRAFT_2852 at 154.499 kb on - strand at 154.499 kb on - strand at 154.499 kb on - strand at 154.503 kb on - strand at 154.632 kb on - strand at 154.821 kb on + strand at 154.821 kb on + strand at 154.822 kb on - strand at 154.822 kb on - strand at 154.822 kb on - strand at 154.822 kb on - strand at 154.822 kb on - strand at 154.886 kb on + strand, within EX28DRAFT_2853 at 154.886 kb on + strand, within EX28DRAFT_2853 at 154.887 kb on - strand, within EX28DRAFT_2853 at 154.887 kb on - strand, within EX28DRAFT_2853 at 154.887 kb on - strand, within EX28DRAFT_2853 at 154.890 kb on + strand, within EX28DRAFT_2853 at 154.890 kb on + strand, within EX28DRAFT_2853 at 154.941 kb on - strand, within EX28DRAFT_2853 at 154.956 kb on + strand, within EX28DRAFT_2853 at 154.957 kb on - strand, within EX28DRAFT_2853 at 155.059 kb on + strand, within EX28DRAFT_2853 at 155.060 kb on - strand, within EX28DRAFT_2853 at 156.003 kb on + strand, within EX28DRAFT_2853 at 156.173 kb on - strand, within EX28DRAFT_2853 at 156.533 kb on + strand at 156.533 kb on + strand at 156.534 kb on - strand at 156.555 kb on - strand at 156.561 kb on + strand at 156.561 kb on + strand at 156.578 kb on + strand at 156.585 kb on + strand at 156.608 kb on + strand at 156.609 kb on - strand at 156.617 kb on + strand at 156.618 kb on - strand at 156.718 kb on + strand at 156.746 kb on + strand at 156.746 kb on + strand at 156.853 kb on + strand, within EX28DRAFT_2854 at 156.854 kb on - strand, within EX28DRAFT_2854 at 156.854 kb on - strand, within EX28DRAFT_2854 at 156.974 kb on + strand, within EX28DRAFT_2854 at 156.975 kb on - strand, within EX28DRAFT_2854 at 156.975 kb on - strand, within EX28DRAFT_2854 at 157.127 kb on - strand at 157.127 kb on - strand at 157.130 kb on + strand at 157.239 kb on - strand at 157.338 kb on + strand, within EX28DRAFT_2855 at 157.427 kb on + strand, within EX28DRAFT_2855 at 157.461 kb on + strand, within EX28DRAFT_2855 at 157.462 kb on - strand, within EX28DRAFT_2855
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 153,745 + EX28DRAFT_2852 0.46 -2.2 153,746 - EX28DRAFT_2852 0.46 -4.2 153,746 - EX28DRAFT_2852 0.46 -3.7 153,746 - EX28DRAFT_2852 0.46 -1.0 153,746 - EX28DRAFT_2852 0.46 +0.3 153,746 - EX28DRAFT_2852 0.46 -5.2 153,872 - EX28DRAFT_2852 0.53 -1.6 153,923 + EX28DRAFT_2852 0.56 -2.2 154,039 + EX28DRAFT_2852 0.63 -3.3 154,040 - EX28DRAFT_2852 0.63 -2.0 154,159 + EX28DRAFT_2852 0.70 -3.7 154,345 + EX28DRAFT_2852 0.81 -4.8 154,499 - -3.4 154,499 - -2.6 154,499 - -3.3 154,503 - -2.3 154,632 - -1.4 154,821 + +0.0 154,821 + -6.0 154,822 - -2.8 154,822 - -2.4 154,822 - -0.8 154,822 - -4.0 154,822 - -2.4 154,886 + EX28DRAFT_2853 0.12 -2.3 154,886 + EX28DRAFT_2853 0.12 -4.0 154,887 - EX28DRAFT_2853 0.12 -5.8 154,887 - EX28DRAFT_2853 0.12 -2.4 154,887 - EX28DRAFT_2853 0.12 -3.8 154,890 + EX28DRAFT_2853 0.12 -4.6 154,890 + EX28DRAFT_2853 0.12 -4.5 154,941 - EX28DRAFT_2853 0.15 -0.9 154,956 + EX28DRAFT_2853 0.16 -2.1 154,957 - EX28DRAFT_2853 0.16 +0.0 155,059 + EX28DRAFT_2853 0.22 -2.4 155,060 - EX28DRAFT_2853 0.22 -3.0 156,003 + EX28DRAFT_2853 0.74 -6.4 156,173 - EX28DRAFT_2853 0.83 -2.1 156,533 + -5.3 156,533 + -3.0 156,534 - -1.9 156,555 - -1.5 156,561 + -1.7 156,561 + -0.4 156,578 + -2.0 156,585 + -3.7 156,608 + -3.3 156,609 - -0.3 156,617 + +0.6 156,618 - -0.2 156,718 + -0.8 156,746 + -0.5 156,746 + +0.4 156,853 + EX28DRAFT_2854 0.20 -0.3 156,854 - EX28DRAFT_2854 0.20 +0.3 156,854 - EX28DRAFT_2854 0.20 +0.2 156,974 + EX28DRAFT_2854 0.53 -0.2 156,975 - EX28DRAFT_2854 0.53 -0.7 156,975 - EX28DRAFT_2854 0.53 -0.4 157,127 - -0.4 157,127 - -1.6 157,130 + +0.1 157,239 - -0.7 157,338 + EX28DRAFT_2855 0.16 -0.0 157,427 + EX28DRAFT_2855 0.29 -1.2 157,461 + EX28DRAFT_2855 0.34 -0.2 157,462 - EX28DRAFT_2855 0.35 -0.6
Or see this region's nucleotide sequence