Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2696 and EX28DRAFT_2697 are separated by 274 nucleotides EX28DRAFT_2697 and EX28DRAFT_2698 are separated by 110 nucleotides EX28DRAFT_2698 and EX28DRAFT_2699 are separated by 30 nucleotides EX28DRAFT_2699 and EX28DRAFT_2700 overlap by 4 nucleotides EX28DRAFT_2700 and EX28DRAFT_2701 are separated by 60 nucleotides
EX28DRAFT_2696: EX28DRAFT_2696 - Beta-barrel assembly machine subunit BamD, at 5,041 to 5,778
_2696
EX28DRAFT_2697: EX28DRAFT_2697 - ribosomal subunit interface protein, at 6,053 to 6,394
_2697
EX28DRAFT_2698: EX28DRAFT_2698 - RNA, at 6,505 to 6,630
_2698
EX28DRAFT_2699: EX28DRAFT_2699 - chorismate mutase (EC 5.4.99.5), at 6,661 to 7,821
_2699
EX28DRAFT_2700: EX28DRAFT_2700 - Gluconolactonase, at 7,818 to 8,690
_2700
EX28DRAFT_2701: EX28DRAFT_2701 - chorismate mutase (EC 5.4.99.5), at 8,751 to 9,872
_2701
Position (kb)
6
7
8 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 5.839 kb on - strand at 5.883 kb on - strand at 6.002 kb on + strand at 6.003 kb on - strand at 6.046 kb on - strand at 6.212 kb on - strand, within EX28DRAFT_2697 at 6.212 kb on - strand, within EX28DRAFT_2697 at 6.212 kb on - strand, within EX28DRAFT_2697 at 6.212 kb on - strand, within EX28DRAFT_2697 at 6.217 kb on + strand, within EX28DRAFT_2697 at 6.217 kb on + strand, within EX28DRAFT_2697 at 6.260 kb on - strand, within EX28DRAFT_2697 at 6.263 kb on + strand, within EX28DRAFT_2697 at 6.264 kb on - strand, within EX28DRAFT_2697 at 6.392 kb on + strand at 6.392 kb on + strand at 6.393 kb on - strand at 6.393 kb on - strand at 6.629 kb on - strand at 6.640 kb on + strand at 6.660 kb on + strand at 6.660 kb on + strand at 6.660 kb on + strand at 6.661 kb on - strand at 6.661 kb on - strand at 6.874 kb on + strand, within EX28DRAFT_2699 at 6.875 kb on - strand, within EX28DRAFT_2699 at 6.952 kb on - strand, within EX28DRAFT_2699 at 7.004 kb on - strand, within EX28DRAFT_2699 at 7.027 kb on + strand, within EX28DRAFT_2699 at 7.028 kb on - strand, within EX28DRAFT_2699 at 7.053 kb on + strand, within EX28DRAFT_2699 at 7.168 kb on + strand, within EX28DRAFT_2699 at 7.169 kb on - strand, within EX28DRAFT_2699 at 7.441 kb on + strand, within EX28DRAFT_2699 at 7.455 kb on - strand, within EX28DRAFT_2699 at 7.687 kb on + strand, within EX28DRAFT_2699 at 7.687 kb on + strand, within EX28DRAFT_2699 at 7.688 kb on - strand, within EX28DRAFT_2699 at 7.688 kb on - strand, within EX28DRAFT_2699 at 7.820 kb on - strand at 7.894 kb on + strand at 7.919 kb on + strand, within EX28DRAFT_2700 at 7.940 kb on + strand, within EX28DRAFT_2700 at 7.941 kb on - strand, within EX28DRAFT_2700 at 8.327 kb on - strand, within EX28DRAFT_2700 at 8.391 kb on - strand, within EX28DRAFT_2700 at 8.475 kb on - strand, within EX28DRAFT_2700 at 8.608 kb on + strand at 8.663 kb on - strand at 8.663 kb on - strand at 8.736 kb on - strand at 8.739 kb on + strand at 8.739 kb on + strand at 8.739 kb on + strand at 8.739 kb on + strand at 8.739 kb on + strand at 8.740 kb on - strand at 8.740 kb on - strand at 8.740 kb on - strand at 8.740 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 5,839 - +0.1 5,883 - +0.3 6,002 + -0.1 6,003 - -0.1 6,046 - -0.2 6,212 - EX28DRAFT_2697 0.46 +0.0 6,212 - EX28DRAFT_2697 0.46 +1.0 6,212 - EX28DRAFT_2697 0.46 +0.0 6,212 - EX28DRAFT_2697 0.46 +0.6 6,217 + EX28DRAFT_2697 0.48 +0.4 6,217 + EX28DRAFT_2697 0.48 +0.2 6,260 - EX28DRAFT_2697 0.61 +0.4 6,263 + EX28DRAFT_2697 0.61 +0.1 6,264 - EX28DRAFT_2697 0.62 -0.2 6,392 + +0.5 6,392 + -0.1 6,393 - +1.3 6,393 - +0.0 6,629 - -3.6 6,640 + +0.0 6,660 + -4.9 6,660 + -4.2 6,660 + -4.1 6,661 - -4.6 6,661 - -5.0 6,874 + EX28DRAFT_2699 0.18 -1.8 6,875 - EX28DRAFT_2699 0.18 -5.6 6,952 - EX28DRAFT_2699 0.25 +0.0 7,004 - EX28DRAFT_2699 0.30 -2.8 7,027 + EX28DRAFT_2699 0.32 -5.1 7,028 - EX28DRAFT_2699 0.32 -4.0 7,053 + EX28DRAFT_2699 0.34 -3.9 7,168 + EX28DRAFT_2699 0.44 -3.4 7,169 - EX28DRAFT_2699 0.44 -2.8 7,441 + EX28DRAFT_2699 0.67 -4.8 7,455 - EX28DRAFT_2699 0.68 -3.6 7,687 + EX28DRAFT_2699 0.88 -6.2 7,687 + EX28DRAFT_2699 0.88 -1.0 7,688 - EX28DRAFT_2699 0.88 -3.0 7,688 - EX28DRAFT_2699 0.88 -2.7 7,820 - -0.6 7,894 + +0.0 7,919 + EX28DRAFT_2700 0.12 +0.6 7,940 + EX28DRAFT_2700 0.14 +0.6 7,941 - EX28DRAFT_2700 0.14 -0.3 8,327 - EX28DRAFT_2700 0.58 +0.3 8,391 - EX28DRAFT_2700 0.66 +0.7 8,475 - EX28DRAFT_2700 0.75 -0.3 8,608 + +0.1 8,663 - +0.6 8,663 - -0.3 8,736 - +0.0 8,739 + +0.6 8,739 + -0.3 8,739 + -0.0 8,739 + -0.5 8,739 + -0.0 8,740 - +0.5 8,740 - +0.4 8,740 - -1.1 8,740 - +0.9
Or see this region's nucleotide sequence