Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2648 and EX28DRAFT_2649 are separated by 163 nucleotides EX28DRAFT_2649 and EX28DRAFT_2650 are separated by 111 nucleotides EX28DRAFT_2650 and EX28DRAFT_2651 are separated by 11 nucleotides
EX28DRAFT_2648: EX28DRAFT_2648 - Integrase, at 2,685,568 to 2,686,743
_2648
EX28DRAFT_2649: EX28DRAFT_2649 - tRNA, at 2,686,907 to 2,686,982
_2649
EX28DRAFT_2650: EX28DRAFT_2650 - glutamate-5-semialdehyde dehydrogenase (EC 1.2.1.41), at 2,687,094 to 2,688,347
_2650
EX28DRAFT_2651: EX28DRAFT_2651 - glutamate 5-kinase, at 2,688,359 to 2,689,462
_2651
Position (kb)
2687
2688
2689 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2686.109 kb on - strand, within EX28DRAFT_2648 at 2686.150 kb on + strand, within EX28DRAFT_2648 at 2686.168 kb on + strand, within EX28DRAFT_2648 at 2686.315 kb on + strand, within EX28DRAFT_2648 at 2686.340 kb on - strand, within EX28DRAFT_2648 at 2686.340 kb on - strand, within EX28DRAFT_2648 at 2686.344 kb on - strand, within EX28DRAFT_2648 at 2686.654 kb on + strand at 2686.654 kb on + strand at 2686.656 kb on + strand at 2686.656 kb on + strand at 2686.690 kb on - strand at 2686.691 kb on + strand at 2686.699 kb on - strand at 2686.776 kb on - strand at 2686.777 kb on + strand at 2686.778 kb on - strand at 2686.974 kb on + strand, within EX28DRAFT_2649 at 2686.974 kb on + strand, within EX28DRAFT_2649 at 2686.974 kb on + strand, within EX28DRAFT_2649 at 2686.975 kb on - strand at 2687.024 kb on + strand at 2687.030 kb on + strand at 2687.031 kb on - strand at 2687.092 kb on - strand at 2687.108 kb on + strand at 2687.134 kb on + strand at 2687.436 kb on + strand, within EX28DRAFT_2650 at 2687.580 kb on + strand, within EX28DRAFT_2650 at 2687.588 kb on + strand, within EX28DRAFT_2650 at 2687.625 kb on - strand, within EX28DRAFT_2650 at 2687.771 kb on - strand, within EX28DRAFT_2650 at 2687.829 kb on + strand, within EX28DRAFT_2650 at 2687.928 kb on - strand, within EX28DRAFT_2650 at 2687.992 kb on + strand, within EX28DRAFT_2650 at 2687.992 kb on + strand, within EX28DRAFT_2650 at 2687.992 kb on + strand, within EX28DRAFT_2650 at 2687.993 kb on - strand, within EX28DRAFT_2650 at 2688.403 kb on - strand at 2688.483 kb on - strand, within EX28DRAFT_2651 at 2689.182 kb on + strand, within EX28DRAFT_2651 at 2689.182 kb on + strand, within EX28DRAFT_2651 at 2689.183 kb on - strand, within EX28DRAFT_2651 at 2689.331 kb on - strand, within EX28DRAFT_2651 at 2689.331 kb on - strand, within EX28DRAFT_2651 at 2689.331 kb on - strand, within EX28DRAFT_2651
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,686,109 - EX28DRAFT_2648 0.46 +0.3 2,686,150 + EX28DRAFT_2648 0.49 +0.5 2,686,168 + EX28DRAFT_2648 0.51 -1.3 2,686,315 + EX28DRAFT_2648 0.64 +0.6 2,686,340 - EX28DRAFT_2648 0.66 -0.0 2,686,340 - EX28DRAFT_2648 0.66 +0.1 2,686,344 - EX28DRAFT_2648 0.66 -0.2 2,686,654 + -0.2 2,686,654 + +1.1 2,686,656 + -0.2 2,686,656 + +0.2 2,686,690 - -6.0 2,686,691 + +0.4 2,686,699 - -0.1 2,686,776 - +0.3 2,686,777 + -0.2 2,686,778 - -0.6 2,686,974 + EX28DRAFT_2649 0.88 -2.5 2,686,974 + EX28DRAFT_2649 0.88 -0.6 2,686,974 + EX28DRAFT_2649 0.88 +0.0 2,686,975 - +0.9 2,687,024 + +0.1 2,687,030 + -0.5 2,687,031 - +0.8 2,687,092 - +0.8 2,687,108 + -4.4 2,687,134 + -2.1 2,687,436 + EX28DRAFT_2650 0.27 -5.1 2,687,580 + EX28DRAFT_2650 0.39 -4.7 2,687,588 + EX28DRAFT_2650 0.39 -5.1 2,687,625 - EX28DRAFT_2650 0.42 -2.4 2,687,771 - EX28DRAFT_2650 0.54 -3.4 2,687,829 + EX28DRAFT_2650 0.59 -3.8 2,687,928 - EX28DRAFT_2650 0.67 -4.2 2,687,992 + EX28DRAFT_2650 0.72 -6.6 2,687,992 + EX28DRAFT_2650 0.72 -2.4 2,687,992 + EX28DRAFT_2650 0.72 -6.3 2,687,993 - EX28DRAFT_2650 0.72 -3.5 2,688,403 - -4.0 2,688,483 - EX28DRAFT_2651 0.11 -4.4 2,689,182 + EX28DRAFT_2651 0.75 -5.9 2,689,182 + EX28DRAFT_2651 0.75 -5.1 2,689,183 - EX28DRAFT_2651 0.75 -2.1 2,689,331 - EX28DRAFT_2651 0.88 -4.1 2,689,331 - EX28DRAFT_2651 0.88 -4.6 2,689,331 - EX28DRAFT_2651 0.88 -6.4
Or see this region's nucleotide sequence