Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2525 and EX28DRAFT_2526 are separated by 66 nucleotides EX28DRAFT_2526 and EX28DRAFT_2527 are separated by 60 nucleotides EX28DRAFT_2527 and EX28DRAFT_2528 are separated by 99 nucleotides EX28DRAFT_2528 and EX28DRAFT_2529 are separated by 148 nucleotides
EX28DRAFT_2525: EX28DRAFT_2525 - ABC-type uncharacterized transport system, periplasmic component, at 2,555,863 to 2,557,563
_2525
EX28DRAFT_2526: EX28DRAFT_2526 - queuosine biosynthesis protein QueC, at 2,557,630 to 2,558,325
_2526
EX28DRAFT_2527: EX28DRAFT_2527 - acyl-CoA thioester hydrolase, YbgC/YbaW family, at 2,558,386 to 2,558,784
_2527
EX28DRAFT_2528: EX28DRAFT_2528 - competence protein ComEA helix-hairpin-helix repeat region, at 2,558,884 to 2,559,264
_2528
EX28DRAFT_2529: EX28DRAFT_2529 - Parvulin-like peptidyl-prolyl isomerase, at 2,559,413 to 2,561,284
_2529
Position (kb)
2558
2559 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2557.389 kb on + strand, within EX28DRAFT_2525 at 2557.460 kb on - strand at 2557.460 kb on - strand at 2557.497 kb on + strand at 2557.497 kb on + strand at 2557.497 kb on + strand at 2557.498 kb on - strand at 2557.498 kb on - strand at 2557.499 kb on + strand at 2557.598 kb on + strand at 2557.613 kb on - strand at 2557.674 kb on + strand at 2557.675 kb on - strand at 2557.703 kb on - strand, within EX28DRAFT_2526 at 2557.827 kb on - strand, within EX28DRAFT_2526 at 2557.863 kb on + strand, within EX28DRAFT_2526 at 2557.863 kb on + strand, within EX28DRAFT_2526 at 2557.864 kb on - strand, within EX28DRAFT_2526 at 2557.864 kb on - strand, within EX28DRAFT_2526 at 2557.879 kb on + strand, within EX28DRAFT_2526 at 2557.879 kb on + strand, within EX28DRAFT_2526 at 2557.880 kb on - strand, within EX28DRAFT_2526 at 2557.880 kb on - strand, within EX28DRAFT_2526 at 2557.929 kb on + strand, within EX28DRAFT_2526 at 2557.957 kb on + strand, within EX28DRAFT_2526 at 2557.958 kb on - strand, within EX28DRAFT_2526 at 2557.958 kb on - strand, within EX28DRAFT_2526 at 2557.958 kb on - strand, within EX28DRAFT_2526 at 2557.958 kb on - strand, within EX28DRAFT_2526 at 2557.971 kb on - strand, within EX28DRAFT_2526 at 2558.194 kb on + strand, within EX28DRAFT_2526 at 2558.319 kb on + strand at 2558.320 kb on - strand at 2558.323 kb on + strand at 2558.329 kb on + strand at 2558.527 kb on + strand, within EX28DRAFT_2527 at 2558.528 kb on - strand, within EX28DRAFT_2527 at 2558.810 kb on + strand at 2558.882 kb on - strand at 2558.885 kb on + strand at 2558.885 kb on + strand at 2558.885 kb on + strand at 2558.886 kb on - strand at 2558.975 kb on + strand, within EX28DRAFT_2528 at 2558.975 kb on + strand, within EX28DRAFT_2528 at 2559.066 kb on + strand, within EX28DRAFT_2528 at 2559.270 kb on + strand at 2559.271 kb on - strand at 2559.271 kb on - strand at 2559.313 kb on + strand at 2559.410 kb on + strand at 2559.411 kb on - strand at 2559.411 kb on - strand at 2559.411 kb on - strand at 2559.414 kb on + strand at 2559.414 kb on + strand at 2559.415 kb on - strand at 2559.415 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,557,389 + EX28DRAFT_2525 0.90 +0.2 2,557,460 - -0.5 2,557,460 - -1.4 2,557,497 + +0.7 2,557,497 + -3.5 2,557,497 + -1.1 2,557,498 - -0.4 2,557,498 - -0.8 2,557,499 + +0.5 2,557,598 + -0.3 2,557,613 - -1.2 2,557,674 + +0.1 2,557,675 - -0.5 2,557,703 - EX28DRAFT_2526 0.10 -1.7 2,557,827 - EX28DRAFT_2526 0.28 +0.1 2,557,863 + EX28DRAFT_2526 0.33 -0.2 2,557,863 + EX28DRAFT_2526 0.33 -1.0 2,557,864 - EX28DRAFT_2526 0.34 +0.2 2,557,864 - EX28DRAFT_2526 0.34 -0.3 2,557,879 + EX28DRAFT_2526 0.36 +1.1 2,557,879 + EX28DRAFT_2526 0.36 +1.1 2,557,880 - EX28DRAFT_2526 0.36 -0.4 2,557,880 - EX28DRAFT_2526 0.36 +0.8 2,557,929 + EX28DRAFT_2526 0.43 +0.6 2,557,957 + EX28DRAFT_2526 0.47 -1.0 2,557,958 - EX28DRAFT_2526 0.47 +1.0 2,557,958 - EX28DRAFT_2526 0.47 +0.1 2,557,958 - EX28DRAFT_2526 0.47 +0.2 2,557,958 - EX28DRAFT_2526 0.47 -0.1 2,557,971 - EX28DRAFT_2526 0.49 -0.1 2,558,194 + EX28DRAFT_2526 0.81 +0.5 2,558,319 + -0.3 2,558,320 - -0.1 2,558,323 + -1.3 2,558,329 + -0.8 2,558,527 + EX28DRAFT_2527 0.35 -1.5 2,558,528 - EX28DRAFT_2527 0.36 -0.6 2,558,810 + +0.4 2,558,882 - +0.3 2,558,885 + +0.9 2,558,885 + +0.5 2,558,885 + -1.4 2,558,886 - -2.0 2,558,975 + EX28DRAFT_2528 0.24 +0.1 2,558,975 + EX28DRAFT_2528 0.24 +0.1 2,559,066 + EX28DRAFT_2528 0.48 -0.6 2,559,270 + -1.5 2,559,271 - +0.4 2,559,271 - -0.2 2,559,313 + -0.3 2,559,410 + -2.1 2,559,411 - -0.4 2,559,411 - +0.9 2,559,411 - +0.1 2,559,414 + -2.4 2,559,414 + -2.9 2,559,415 - -0.2 2,559,415 - -0.8
Or see this region's nucleotide sequence