Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2490 and EX28DRAFT_2491 are separated by 50 nucleotides EX28DRAFT_2491 and EX28DRAFT_2492 are separated by 14 nucleotides EX28DRAFT_2492 and EX28DRAFT_2493 are separated by 54 nucleotides
EX28DRAFT_2490: EX28DRAFT_2490 - Uncharacterized protein conserved in bacteria, at 2,520,289 to 2,520,666
_2490
EX28DRAFT_2491: EX28DRAFT_2491 - restart primosome assembly protein PriC, at 2,520,717 to 2,521,244
_2491
EX28DRAFT_2492: EX28DRAFT_2492 - Protein of unknown function (DUF2496), at 2,521,259 to 2,521,417
_2492
EX28DRAFT_2493: EX28DRAFT_2493 - Small-conductance mechanosensitive channel, at 2,521,472 to 2,524,819
_2493
Position (kb)
2521
2522 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2520.340 kb on - strand, within EX28DRAFT_2490 at 2520.409 kb on - strand, within EX28DRAFT_2490 at 2520.513 kb on - strand, within EX28DRAFT_2490 at 2520.654 kb on + strand at 2520.700 kb on + strand at 2520.779 kb on + strand, within EX28DRAFT_2491 at 2520.861 kb on + strand, within EX28DRAFT_2491 at 2520.861 kb on + strand, within EX28DRAFT_2491 at 2521.147 kb on + strand, within EX28DRAFT_2491 at 2521.148 kb on - strand, within EX28DRAFT_2491 at 2521.309 kb on + strand, within EX28DRAFT_2492 at 2521.351 kb on + strand, within EX28DRAFT_2492 at 2521.466 kb on - strand at 2521.519 kb on + strand at 2521.520 kb on - strand at 2521.520 kb on - strand at 2521.520 kb on - strand at 2521.576 kb on + strand at 2521.577 kb on - strand at 2521.577 kb on - strand at 2521.632 kb on + strand at 2521.661 kb on + strand at 2521.797 kb on + strand at 2521.798 kb on - strand at 2522.146 kb on + strand, within EX28DRAFT_2493 at 2522.147 kb on - strand, within EX28DRAFT_2493 at 2522.157 kb on + strand, within EX28DRAFT_2493 at 2522.157 kb on + strand, within EX28DRAFT_2493 at 2522.157 kb on + strand, within EX28DRAFT_2493 at 2522.158 kb on - strand, within EX28DRAFT_2493 at 2522.346 kb on + strand, within EX28DRAFT_2493 at 2522.346 kb on + strand, within EX28DRAFT_2493 at 2522.347 kb on - strand, within EX28DRAFT_2493 at 2522.363 kb on - strand, within EX28DRAFT_2493 at 2522.373 kb on + strand, within EX28DRAFT_2493 at 2522.390 kb on - strand, within EX28DRAFT_2493
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,520,340 - EX28DRAFT_2490 0.13 +0.7 2,520,409 - EX28DRAFT_2490 0.32 -0.2 2,520,513 - EX28DRAFT_2490 0.59 -0.1 2,520,654 + +0.2 2,520,700 + -0.2 2,520,779 + EX28DRAFT_2491 0.12 -1.2 2,520,861 + EX28DRAFT_2491 0.27 +0.1 2,520,861 + EX28DRAFT_2491 0.27 +1.1 2,521,147 + EX28DRAFT_2491 0.81 -2.1 2,521,148 - EX28DRAFT_2491 0.82 +0.2 2,521,309 + EX28DRAFT_2492 0.31 -1.1 2,521,351 + EX28DRAFT_2492 0.58 -2.9 2,521,466 - -0.1 2,521,519 + +0.0 2,521,520 - +0.4 2,521,520 - +0.1 2,521,520 - -1.3 2,521,576 + -0.9 2,521,577 - +0.3 2,521,577 - -0.1 2,521,632 + +0.1 2,521,661 + +0.4 2,521,797 + +2.2 2,521,798 - -0.9 2,522,146 + EX28DRAFT_2493 0.20 -0.3 2,522,147 - EX28DRAFT_2493 0.20 +0.0 2,522,157 + EX28DRAFT_2493 0.20 -2.7 2,522,157 + EX28DRAFT_2493 0.20 +0.8 2,522,157 + EX28DRAFT_2493 0.20 -0.7 2,522,158 - EX28DRAFT_2493 0.20 +0.5 2,522,346 + EX28DRAFT_2493 0.26 +1.2 2,522,346 + EX28DRAFT_2493 0.26 +0.1 2,522,347 - EX28DRAFT_2493 0.26 +0.3 2,522,363 - EX28DRAFT_2493 0.27 -0.2 2,522,373 + EX28DRAFT_2493 0.27 -1.4 2,522,390 - EX28DRAFT_2493 0.27 +0.3
Or see this region's nucleotide sequence