Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2353 and EX28DRAFT_2354 are separated by 122 nucleotides EX28DRAFT_2354 and EX28DRAFT_2355 overlap by 4 nucleotides EX28DRAFT_2355 and EX28DRAFT_2356 overlap by 4 nucleotides EX28DRAFT_2356 and EX28DRAFT_2357 are separated by 59 nucleotides
EX28DRAFT_2353: EX28DRAFT_2353 - Aspartate/tyrosine/aromatic aminotransferase, at 2,374,084 to 2,375,244
_2353
EX28DRAFT_2354: EX28DRAFT_2354 - methylthioribulose-1-phosphate dehydratase (EC 4.2.1.109), at 2,375,367 to 2,375,981
_2354
EX28DRAFT_2355: EX28DRAFT_2355 - 2,3-diketo-5-methylthio-1-phosphopentane phosphatase, at 2,375,978 to 2,376,667
_2355
EX28DRAFT_2356: EX28DRAFT_2356 - Uncharacterized conserved protein, contains double-stranded beta-helix domain, at 2,376,664 to 2,377,206
_2356
EX28DRAFT_2357: EX28DRAFT_2357 - Uncharacterized conserved protein, at 2,377,266 to 2,377,736
_2357
Position (kb)
2375
2376
2377 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2375.085 kb on - strand, within EX28DRAFT_2353 at 2375.101 kb on + strand, within EX28DRAFT_2353 at 2375.101 kb on + strand, within EX28DRAFT_2353 at 2375.102 kb on - strand, within EX28DRAFT_2353 at 2375.102 kb on - strand, within EX28DRAFT_2353 at 2375.102 kb on - strand, within EX28DRAFT_2353 at 2375.193 kb on + strand at 2375.262 kb on + strand at 2375.269 kb on + strand at 2375.269 kb on + strand at 2375.270 kb on - strand at 2375.319 kb on + strand at 2375.334 kb on + strand at 2375.334 kb on + strand at 2375.453 kb on + strand, within EX28DRAFT_2354 at 2375.610 kb on - strand, within EX28DRAFT_2354 at 2375.610 kb on - strand, within EX28DRAFT_2354 at 2375.846 kb on + strand, within EX28DRAFT_2354 at 2375.846 kb on + strand, within EX28DRAFT_2354 at 2375.847 kb on - strand, within EX28DRAFT_2354 at 2375.878 kb on + strand, within EX28DRAFT_2354 at 2375.929 kb on + strand at 2375.930 kb on - strand at 2375.939 kb on - strand at 2376.193 kb on + strand, within EX28DRAFT_2355 at 2376.297 kb on + strand, within EX28DRAFT_2355 at 2376.409 kb on + strand, within EX28DRAFT_2355 at 2376.429 kb on - strand, within EX28DRAFT_2355 at 2376.682 kb on + strand at 2376.798 kb on - strand, within EX28DRAFT_2356 at 2376.954 kb on + strand, within EX28DRAFT_2356 at 2377.030 kb on + strand, within EX28DRAFT_2356 at 2377.030 kb on + strand, within EX28DRAFT_2356 at 2377.030 kb on + strand, within EX28DRAFT_2356 at 2377.116 kb on + strand, within EX28DRAFT_2356 at 2377.128 kb on + strand, within EX28DRAFT_2356 at 2377.129 kb on - strand, within EX28DRAFT_2356 at 2377.204 kb on + strand at 2377.205 kb on - strand at 2377.205 kb on - strand at 2377.288 kb on + strand at 2377.289 kb on - strand at 2377.473 kb on - strand, within EX28DRAFT_2357 at 2377.522 kb on - strand, within EX28DRAFT_2357
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,375,085 - EX28DRAFT_2353 0.86 +0.4 2,375,101 + EX28DRAFT_2353 0.88 +0.6 2,375,101 + EX28DRAFT_2353 0.88 -2.5 2,375,102 - EX28DRAFT_2353 0.88 -3.1 2,375,102 - EX28DRAFT_2353 0.88 +0.4 2,375,102 - EX28DRAFT_2353 0.88 +0.2 2,375,193 + +2.1 2,375,262 + +0.5 2,375,269 + +0.5 2,375,269 + -1.1 2,375,270 - +0.3 2,375,319 + +0.0 2,375,334 + -0.0 2,375,334 + -0.1 2,375,453 + EX28DRAFT_2354 0.14 -0.3 2,375,610 - EX28DRAFT_2354 0.40 -0.5 2,375,610 - EX28DRAFT_2354 0.40 +0.3 2,375,846 + EX28DRAFT_2354 0.78 +0.1 2,375,846 + EX28DRAFT_2354 0.78 +0.2 2,375,847 - EX28DRAFT_2354 0.78 -2.1 2,375,878 + EX28DRAFT_2354 0.83 +0.6 2,375,929 + -0.9 2,375,930 - +0.3 2,375,939 - -0.4 2,376,193 + EX28DRAFT_2355 0.31 -0.1 2,376,297 + EX28DRAFT_2355 0.46 +0.3 2,376,409 + EX28DRAFT_2355 0.62 +2.3 2,376,429 - EX28DRAFT_2355 0.65 -0.1 2,376,682 + +0.1 2,376,798 - EX28DRAFT_2356 0.25 +0.4 2,376,954 + EX28DRAFT_2356 0.53 -0.4 2,377,030 + EX28DRAFT_2356 0.67 -0.1 2,377,030 + EX28DRAFT_2356 0.67 -1.0 2,377,030 + EX28DRAFT_2356 0.67 +1.8 2,377,116 + EX28DRAFT_2356 0.83 -0.5 2,377,128 + EX28DRAFT_2356 0.85 +0.2 2,377,129 - EX28DRAFT_2356 0.86 +0.5 2,377,204 + +0.3 2,377,205 - -0.3 2,377,205 - -0.5 2,377,288 + -1.4 2,377,289 - -0.2 2,377,473 - EX28DRAFT_2357 0.44 -0.4 2,377,522 - EX28DRAFT_2357 0.54 +0.6
Or see this region's nucleotide sequence