Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2264 and EX28DRAFT_2265 are separated by 83 nucleotides EX28DRAFT_2265 and EX28DRAFT_2266 are separated by 14 nucleotides
EX28DRAFT_2264: EX28DRAFT_2264 - succinyl-CoA synthetase, beta subunit, at 2,286,194 to 2,287,360
_2264
EX28DRAFT_2265: EX28DRAFT_2265 - 2-oxoglutarate dehydrogenase E2 component (EC 2.3.1.61), at 2,287,444 to 2,288,664
_2265
EX28DRAFT_2266: EX28DRAFT_2266 - 2-oxoglutarate dehydrogenase, E1 component, at 2,288,679 to 2,291,486
_2266
Position (kb)
2287
2288
2289 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2286.495 kb on - strand, within EX28DRAFT_2264 at 2286.495 kb on - strand, within EX28DRAFT_2264 at 2286.577 kb on + strand, within EX28DRAFT_2264 at 2286.607 kb on - strand, within EX28DRAFT_2264 at 2287.012 kb on + strand, within EX28DRAFT_2264 at 2287.171 kb on + strand, within EX28DRAFT_2264 at 2287.225 kb on + strand, within EX28DRAFT_2264 at 2287.225 kb on + strand, within EX28DRAFT_2264 at 2287.225 kb on + strand, within EX28DRAFT_2264 at 2287.225 kb on + strand, within EX28DRAFT_2264 at 2287.226 kb on - strand, within EX28DRAFT_2264 at 2287.226 kb on - strand, within EX28DRAFT_2264 at 2287.282 kb on + strand at 2287.283 kb on - strand at 2287.285 kb on + strand at 2287.367 kb on + strand at 2287.390 kb on - strand at 2287.419 kb on + strand at 2287.751 kb on - strand, within EX28DRAFT_2265 at 2287.950 kb on - strand, within EX28DRAFT_2265 at 2288.739 kb on - strand at 2288.739 kb on - strand at 2288.741 kb on - strand at 2288.741 kb on - strand at 2288.884 kb on - strand at 2288.894 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,286,495 - EX28DRAFT_2264 0.26 +0.3 2,286,495 - EX28DRAFT_2264 0.26 -0.4 2,286,577 + EX28DRAFT_2264 0.33 +0.1 2,286,607 - EX28DRAFT_2264 0.35 -2.5 2,287,012 + EX28DRAFT_2264 0.70 -0.1 2,287,171 + EX28DRAFT_2264 0.84 -1.8 2,287,225 + EX28DRAFT_2264 0.88 +0.0 2,287,225 + EX28DRAFT_2264 0.88 -0.7 2,287,225 + EX28DRAFT_2264 0.88 +1.1 2,287,225 + EX28DRAFT_2264 0.88 -0.4 2,287,226 - EX28DRAFT_2264 0.88 -0.9 2,287,226 - EX28DRAFT_2264 0.88 -0.9 2,287,282 + -0.7 2,287,283 - -0.5 2,287,285 + -1.3 2,287,367 + +0.3 2,287,390 - +0.1 2,287,419 + +0.4 2,287,751 - EX28DRAFT_2265 0.25 -5.8 2,287,950 - EX28DRAFT_2265 0.41 -3.7 2,288,739 - -1.1 2,288,739 - -2.7 2,288,741 - -2.9 2,288,741 - -3.8 2,288,884 - -4.3 2,288,894 - -1.7
Or see this region's nucleotide sequence