Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2243 and EX28DRAFT_2244 overlap by 4 nucleotides EX28DRAFT_2244 and EX28DRAFT_2245 are separated by 22 nucleotides EX28DRAFT_2245 and EX28DRAFT_2246 are separated by 222 nucleotides EX28DRAFT_2246 and EX28DRAFT_2247 are separated by 10 nucleotides EX28DRAFT_2247 and EX28DRAFT_2248 are separated by 41 nucleotides EX28DRAFT_2248 and EX28DRAFT_2249 are separated by 161 nucleotides
EX28DRAFT_2243: EX28DRAFT_2243 - cation diffusion facilitator family transporter, at 2,266,805 to 2,267,743
_2243
EX28DRAFT_2244: EX28DRAFT_2244 - nicotinamide mononucleotide transporter PnuC, at 2,267,740 to 2,268,459
_2244
EX28DRAFT_2245: EX28DRAFT_2245 - quinolinate synthetase (EC 2.5.1.72), at 2,268,482 to 2,269,525
_2245
EX28DRAFT_2246: EX28DRAFT_2246 - tRNA, at 2,269,748 to 2,269,823
_2246
EX28DRAFT_2247: EX28DRAFT_2247 - tRNA, at 2,269,834 to 2,269,909
_2247
EX28DRAFT_2248: EX28DRAFT_2248 - tRNA, at 2,269,951 to 2,270,026
_2248
EX28DRAFT_2249: EX28DRAFT_2249 - tol-pal system protein YbgF, at 2,270,188 to 2,270,982
_2249
Position (kb)
2268
2269
2270 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2267.636 kb on + strand, within EX28DRAFT_2243 at 2267.636 kb on + strand, within EX28DRAFT_2243 at 2267.669 kb on + strand at 2267.670 kb on - strand at 2267.781 kb on + strand at 2267.828 kb on + strand, within EX28DRAFT_2244 at 2267.918 kb on + strand, within EX28DRAFT_2244 at 2267.919 kb on - strand, within EX28DRAFT_2244 at 2268.081 kb on + strand, within EX28DRAFT_2244 at 2268.081 kb on + strand, within EX28DRAFT_2244 at 2268.082 kb on - strand, within EX28DRAFT_2244 at 2268.083 kb on + strand, within EX28DRAFT_2244 at 2268.083 kb on + strand, within EX28DRAFT_2244 at 2268.083 kb on + strand, within EX28DRAFT_2244 at 2268.083 kb on + strand, within EX28DRAFT_2244 at 2268.083 kb on + strand, within EX28DRAFT_2244 at 2268.083 kb on + strand, within EX28DRAFT_2244 at 2268.083 kb on + strand, within EX28DRAFT_2244 at 2268.083 kb on + strand, within EX28DRAFT_2244 at 2268.095 kb on - strand, within EX28DRAFT_2244 at 2268.213 kb on - strand, within EX28DRAFT_2244 at 2268.258 kb on - strand, within EX28DRAFT_2244 at 2268.352 kb on - strand, within EX28DRAFT_2244 at 2268.422 kb on - strand at 2268.422 kb on - strand at 2268.423 kb on + strand at 2268.423 kb on + strand at 2268.424 kb on - strand at 2268.424 kb on - strand at 2268.483 kb on + strand at 2268.483 kb on + strand at 2268.483 kb on + strand at 2268.487 kb on + strand at 2268.487 kb on + strand at 2268.487 kb on + strand at 2268.487 kb on + strand at 2268.487 kb on + strand at 2268.488 kb on - strand at 2268.492 kb on - strand at 2268.531 kb on - strand at 2268.724 kb on - strand, within EX28DRAFT_2245 at 2268.787 kb on + strand, within EX28DRAFT_2245 at 2268.975 kb on + strand, within EX28DRAFT_2245 at 2268.976 kb on - strand, within EX28DRAFT_2245 at 2268.976 kb on - strand, within EX28DRAFT_2245 at 2268.976 kb on - strand, within EX28DRAFT_2245 at 2269.006 kb on + strand, within EX28DRAFT_2245 at 2269.007 kb on - strand, within EX28DRAFT_2245 at 2269.199 kb on - strand, within EX28DRAFT_2245 at 2269.256 kb on - strand, within EX28DRAFT_2245 at 2269.333 kb on + strand, within EX28DRAFT_2245 at 2269.380 kb on + strand, within EX28DRAFT_2245 at 2269.579 kb on - strand at 2269.628 kb on - strand at 2270.128 kb on + strand at 2270.128 kb on + strand at 2270.137 kb on - strand at 2270.255 kb on - strand at 2270.315 kb on - strand, within EX28DRAFT_2249 at 2270.315 kb on - strand, within EX28DRAFT_2249 at 2270.401 kb on - strand, within EX28DRAFT_2249 at 2270.411 kb on + strand, within EX28DRAFT_2249 at 2270.411 kb on + strand, within EX28DRAFT_2249 at 2270.411 kb on + strand, within EX28DRAFT_2249 at 2270.412 kb on - strand, within EX28DRAFT_2249 at 2270.412 kb on - strand, within EX28DRAFT_2249 at 2270.412 kb on - strand, within EX28DRAFT_2249 at 2270.432 kb on + strand, within EX28DRAFT_2249 at 2270.450 kb on + strand, within EX28DRAFT_2249 at 2270.466 kb on - strand, within EX28DRAFT_2249
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,267,636 + EX28DRAFT_2243 0.88 +1.1 2,267,636 + EX28DRAFT_2243 0.88 +0.8 2,267,669 + -0.1 2,267,670 - +0.5 2,267,781 + -0.8 2,267,828 + EX28DRAFT_2244 0.12 -1.2 2,267,918 + EX28DRAFT_2244 0.25 -0.5 2,267,919 - EX28DRAFT_2244 0.25 +0.2 2,268,081 + EX28DRAFT_2244 0.47 +1.0 2,268,081 + EX28DRAFT_2244 0.47 +1.0 2,268,082 - EX28DRAFT_2244 0.47 -1.0 2,268,083 + EX28DRAFT_2244 0.48 -0.2 2,268,083 + EX28DRAFT_2244 0.48 -0.2 2,268,083 + EX28DRAFT_2244 0.48 +0.4 2,268,083 + EX28DRAFT_2244 0.48 -0.1 2,268,083 + EX28DRAFT_2244 0.48 -0.4 2,268,083 + EX28DRAFT_2244 0.48 +0.2 2,268,083 + EX28DRAFT_2244 0.48 -0.4 2,268,083 + EX28DRAFT_2244 0.48 +0.3 2,268,095 - EX28DRAFT_2244 0.49 -0.6 2,268,213 - EX28DRAFT_2244 0.66 +1.2 2,268,258 - EX28DRAFT_2244 0.72 +0.4 2,268,352 - EX28DRAFT_2244 0.85 -1.1 2,268,422 - +0.6 2,268,422 - -0.2 2,268,423 + -0.4 2,268,423 + -0.5 2,268,424 - -0.5 2,268,424 - +0.6 2,268,483 + -1.3 2,268,483 + +0.4 2,268,483 + -0.5 2,268,487 + -2.5 2,268,487 + +0.3 2,268,487 + -0.1 2,268,487 + -0.4 2,268,487 + +0.1 2,268,488 - +1.0 2,268,492 - +0.4 2,268,531 - -0.7 2,268,724 - EX28DRAFT_2245 0.23 -1.4 2,268,787 + EX28DRAFT_2245 0.29 -1.9 2,268,975 + EX28DRAFT_2245 0.47 -2.9 2,268,976 - EX28DRAFT_2245 0.47 -2.1 2,268,976 - EX28DRAFT_2245 0.47 -2.0 2,268,976 - EX28DRAFT_2245 0.47 -1.7 2,269,006 + EX28DRAFT_2245 0.50 -0.8 2,269,007 - EX28DRAFT_2245 0.50 -1.3 2,269,199 - EX28DRAFT_2245 0.69 -3.5 2,269,256 - EX28DRAFT_2245 0.74 -2.7 2,269,333 + EX28DRAFT_2245 0.82 -4.8 2,269,380 + EX28DRAFT_2245 0.86 -1.5 2,269,579 - +0.5 2,269,628 - +0.1 2,270,128 + +0.1 2,270,128 + -0.0 2,270,137 - -0.2 2,270,255 - +0.0 2,270,315 - EX28DRAFT_2249 0.16 -0.1 2,270,315 - EX28DRAFT_2249 0.16 -0.4 2,270,401 - EX28DRAFT_2249 0.27 -1.0 2,270,411 + EX28DRAFT_2249 0.28 +2.1 2,270,411 + EX28DRAFT_2249 0.28 +0.4 2,270,411 + EX28DRAFT_2249 0.28 +0.5 2,270,412 - EX28DRAFT_2249 0.28 -1.4 2,270,412 - EX28DRAFT_2249 0.28 -0.1 2,270,412 - EX28DRAFT_2249 0.28 +0.4 2,270,432 + EX28DRAFT_2249 0.31 -0.0 2,270,450 + EX28DRAFT_2249 0.33 +0.3 2,270,466 - EX28DRAFT_2249 0.35 +0.5
Or see this region's nucleotide sequence