Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2173 and EX28DRAFT_2174 are separated by 299 nucleotides EX28DRAFT_2174 and EX28DRAFT_2175 are separated by 45 nucleotides EX28DRAFT_2175 and EX28DRAFT_2176 are separated by 253 nucleotides
EX28DRAFT_2173: EX28DRAFT_2173 - Predicted permease, DMT superfamily, at 2,197,283 to 2,198,170
_2173
EX28DRAFT_2174: EX28DRAFT_2174 - DNA-binding ferritin-like protein (oxidative damage protectant), at 2,198,470 to 2,198,973
_2174
EX28DRAFT_2175: EX28DRAFT_2175 - hypothetical protein, at 2,199,019 to 2,199,171
_2175
EX28DRAFT_2176: EX28DRAFT_2176 - hypothetical protein, at 2,199,425 to 2,200,156
_2176
Position (kb)
2199
2200 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2198.143 kb on + strand at 2198.169 kb on - strand at 2198.176 kb on + strand at 2198.177 kb on - strand at 2198.179 kb on - strand at 2198.179 kb on - strand at 2198.179 kb on - strand at 2198.265 kb on + strand at 2198.279 kb on + strand at 2198.279 kb on + strand at 2198.280 kb on - strand at 2198.420 kb on - strand at 2198.423 kb on + strand at 2198.424 kb on - strand at 2198.436 kb on - strand at 2198.446 kb on + strand at 2198.447 kb on - strand at 2198.516 kb on - strand at 2198.531 kb on + strand, within EX28DRAFT_2174 at 2198.596 kb on - strand, within EX28DRAFT_2174 at 2198.629 kb on - strand, within EX28DRAFT_2174 at 2198.629 kb on - strand, within EX28DRAFT_2174 at 2198.962 kb on - strand at 2198.971 kb on + strand at 2198.978 kb on + strand at 2198.980 kb on + strand at 2198.980 kb on + strand at 2198.980 kb on + strand at 2198.981 kb on - strand at 2198.981 kb on - strand at 2198.989 kb on + strand at 2199.025 kb on - strand at 2199.054 kb on - strand, within EX28DRAFT_2175 at 2199.106 kb on + strand, within EX28DRAFT_2175 at 2199.106 kb on + strand, within EX28DRAFT_2175 at 2199.178 kb on - strand at 2199.190 kb on - strand at 2199.207 kb on - strand at 2199.207 kb on - strand at 2199.214 kb on + strand at 2199.253 kb on + strand at 2199.274 kb on - strand at 2199.274 kb on - strand at 2199.333 kb on + strand at 2199.334 kb on - strand at 2199.334 kb on - strand at 2199.334 kb on - strand at 2199.446 kb on - strand at 2199.494 kb on + strand at 2199.495 kb on - strand at 2199.529 kb on + strand, within EX28DRAFT_2176 at 2199.529 kb on + strand, within EX28DRAFT_2176 at 2199.529 kb on + strand, within EX28DRAFT_2176 at 2199.530 kb on - strand, within EX28DRAFT_2176 at 2199.533 kb on + strand, within EX28DRAFT_2176 at 2199.533 kb on + strand, within EX28DRAFT_2176 at 2199.533 kb on + strand, within EX28DRAFT_2176 at 2199.533 kb on + strand, within EX28DRAFT_2176 at 2199.534 kb on - strand, within EX28DRAFT_2176 at 2199.544 kb on - strand, within EX28DRAFT_2176 at 2199.591 kb on + strand, within EX28DRAFT_2176 at 2199.592 kb on - strand, within EX28DRAFT_2176 at 2199.592 kb on - strand, within EX28DRAFT_2176 at 2199.592 kb on - strand, within EX28DRAFT_2176 at 2199.764 kb on - strand, within EX28DRAFT_2176 at 2199.781 kb on - strand, within EX28DRAFT_2176 at 2199.805 kb on - strand, within EX28DRAFT_2176 at 2199.848 kb on - strand, within EX28DRAFT_2176 at 2199.850 kb on - strand, within EX28DRAFT_2176 at 2199.850 kb on - strand, within EX28DRAFT_2176 at 2199.850 kb on - strand, within EX28DRAFT_2176 at 2199.850 kb on - strand, within EX28DRAFT_2176 at 2199.850 kb on - strand, within EX28DRAFT_2176 at 2199.875 kb on - strand, within EX28DRAFT_2176 at 2199.894 kb on + strand, within EX28DRAFT_2176 at 2200.063 kb on + strand, within EX28DRAFT_2176 at 2200.064 kb on - strand, within EX28DRAFT_2176 at 2200.109 kb on - strand at 2200.109 kb on - strand at 2200.127 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,198,143 + +1.4 2,198,169 - -0.2 2,198,176 + +1.1 2,198,177 - -0.7 2,198,179 - +0.3 2,198,179 - +0.3 2,198,179 - +0.4 2,198,265 + -0.0 2,198,279 + -0.0 2,198,279 + +0.8 2,198,280 - -0.6 2,198,420 - -0.1 2,198,423 + +0.3 2,198,424 - +0.1 2,198,436 - +1.0 2,198,446 + +0.7 2,198,447 - +0.8 2,198,516 - +0.4 2,198,531 + EX28DRAFT_2174 0.12 +0.0 2,198,596 - EX28DRAFT_2174 0.25 +1.1 2,198,629 - EX28DRAFT_2174 0.32 +1.4 2,198,629 - EX28DRAFT_2174 0.32 +1.6 2,198,962 - +0.5 2,198,971 + +1.1 2,198,978 + +1.0 2,198,980 + +2.6 2,198,980 + +0.0 2,198,980 + -0.1 2,198,981 - +0.1 2,198,981 - -0.2 2,198,989 + -0.3 2,199,025 - -0.9 2,199,054 - EX28DRAFT_2175 0.23 -2.0 2,199,106 + EX28DRAFT_2175 0.57 +0.1 2,199,106 + EX28DRAFT_2175 0.57 -4.3 2,199,178 - +0.7 2,199,190 - -0.0 2,199,207 - -0.9 2,199,207 - +3.1 2,199,214 + +0.8 2,199,253 + +0.2 2,199,274 - -0.0 2,199,274 - +0.3 2,199,333 + -0.2 2,199,334 - -0.2 2,199,334 - -0.8 2,199,334 - -0.0 2,199,446 - -0.4 2,199,494 + +0.1 2,199,495 - +0.2 2,199,529 + EX28DRAFT_2176 0.14 +0.5 2,199,529 + EX28DRAFT_2176 0.14 +1.0 2,199,529 + EX28DRAFT_2176 0.14 -0.2 2,199,530 - EX28DRAFT_2176 0.14 -0.0 2,199,533 + EX28DRAFT_2176 0.15 -0.0 2,199,533 + EX28DRAFT_2176 0.15 +0.5 2,199,533 + EX28DRAFT_2176 0.15 -0.0 2,199,533 + EX28DRAFT_2176 0.15 +0.1 2,199,534 - EX28DRAFT_2176 0.15 -3.0 2,199,544 - EX28DRAFT_2176 0.16 -0.1 2,199,591 + EX28DRAFT_2176 0.23 +0.4 2,199,592 - EX28DRAFT_2176 0.23 -0.3 2,199,592 - EX28DRAFT_2176 0.23 +0.7 2,199,592 - EX28DRAFT_2176 0.23 +0.8 2,199,764 - EX28DRAFT_2176 0.46 +2.7 2,199,781 - EX28DRAFT_2176 0.49 +1.0 2,199,805 - EX28DRAFT_2176 0.52 +1.1 2,199,848 - EX28DRAFT_2176 0.58 +0.1 2,199,850 - EX28DRAFT_2176 0.58 +0.2 2,199,850 - EX28DRAFT_2176 0.58 -2.1 2,199,850 - EX28DRAFT_2176 0.58 +0.8 2,199,850 - EX28DRAFT_2176 0.58 -1.0 2,199,850 - EX28DRAFT_2176 0.58 +1.4 2,199,875 - EX28DRAFT_2176 0.61 +0.3 2,199,894 + EX28DRAFT_2176 0.64 -0.4 2,200,063 + EX28DRAFT_2176 0.87 +1.2 2,200,064 - EX28DRAFT_2176 0.87 +3.3 2,200,109 - -0.5 2,200,109 - +0.3 2,200,127 - -0.1
Or see this region's nucleotide sequence