Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2101 and EX28DRAFT_2102 are separated by 48 nucleotides EX28DRAFT_2102 and EX28DRAFT_2103 are separated by 184 nucleotides EX28DRAFT_2103 and EX28DRAFT_2104 are separated by 30 nucleotides
EX28DRAFT_2101: EX28DRAFT_2101 - alpha-L-glutamate ligase, RimK family, at 2,124,945 to 2,125,868
_2101
EX28DRAFT_2102: EX28DRAFT_2102 - Nitroreductase, at 2,125,917 to 2,126,639
_2102
EX28DRAFT_2103: EX28DRAFT_2103 - Glutaredoxin, GrxA family, at 2,126,824 to 2,127,093
_2103
EX28DRAFT_2104: EX28DRAFT_2104 - Putative inner membrane protein of Enterobacteriaceae, at 2,127,124 to 2,127,498
_2104
Position (kb)
2125
2126
2127 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2125.107 kb on + strand, within EX28DRAFT_2101 at 2125.176 kb on - strand, within EX28DRAFT_2101 at 2125.211 kb on + strand, within EX28DRAFT_2101 at 2125.312 kb on + strand, within EX28DRAFT_2101 at 2125.312 kb on + strand, within EX28DRAFT_2101 at 2125.313 kb on - strand, within EX28DRAFT_2101 at 2125.313 kb on - strand, within EX28DRAFT_2101 at 2125.568 kb on + strand, within EX28DRAFT_2101 at 2125.633 kb on + strand, within EX28DRAFT_2101 at 2125.637 kb on - strand, within EX28DRAFT_2101 at 2125.734 kb on - strand, within EX28DRAFT_2101 at 2125.735 kb on + strand, within EX28DRAFT_2101 at 2125.735 kb on + strand, within EX28DRAFT_2101 at 2125.735 kb on + strand, within EX28DRAFT_2101 at 2125.735 kb on + strand, within EX28DRAFT_2101 at 2125.735 kb on + strand, within EX28DRAFT_2101 at 2125.736 kb on - strand, within EX28DRAFT_2101 at 2125.736 kb on - strand, within EX28DRAFT_2101 at 2125.736 kb on - strand, within EX28DRAFT_2101 at 2125.834 kb on - strand at 2125.888 kb on + strand at 2125.889 kb on - strand at 2125.891 kb on - strand at 2125.891 kb on - strand at 2125.948 kb on + strand at 2125.948 kb on + strand at 2125.959 kb on - strand at 2126.045 kb on - strand, within EX28DRAFT_2102 at 2126.241 kb on - strand, within EX28DRAFT_2102 at 2126.257 kb on + strand, within EX28DRAFT_2102 at 2126.257 kb on + strand, within EX28DRAFT_2102 at 2126.258 kb on - strand, within EX28DRAFT_2102 at 2126.546 kb on + strand, within EX28DRAFT_2102 at 2126.625 kb on + strand at 2126.682 kb on + strand at 2126.683 kb on - strand at 2126.704 kb on - strand at 2126.704 kb on - strand at 2126.704 kb on - strand at 2126.718 kb on - strand at 2126.718 kb on - strand at 2126.723 kb on + strand at 2126.723 kb on + strand at 2126.723 kb on + strand at 2126.724 kb on - strand at 2126.728 kb on + strand at 2126.779 kb on + strand at 2126.779 kb on + strand at 2126.780 kb on - strand at 2126.780 kb on - strand at 2126.780 kb on - strand at 2126.781 kb on + strand at 2126.781 kb on + strand at 2126.782 kb on - strand at 2126.782 kb on - strand at 2126.782 kb on - strand at 2126.782 kb on - strand at 2126.788 kb on + strand at 2126.789 kb on - strand at 2127.091 kb on + strand at 2127.125 kb on + strand at 2127.147 kb on + strand at 2127.156 kb on + strand at 2127.157 kb on - strand at 2127.170 kb on + strand, within EX28DRAFT_2104 at 2127.170 kb on + strand, within EX28DRAFT_2104 at 2127.171 kb on - strand, within EX28DRAFT_2104 at 2127.247 kb on + strand, within EX28DRAFT_2104 at 2127.251 kb on + strand, within EX28DRAFT_2104 at 2127.251 kb on + strand, within EX28DRAFT_2104 at 2127.251 kb on + strand, within EX28DRAFT_2104 at 2127.252 kb on - strand, within EX28DRAFT_2104 at 2127.447 kb on + strand, within EX28DRAFT_2104 at 2127.489 kb on + strand at 2127.489 kb on + strand at 2127.514 kb on + strand at 2127.514 kb on + strand at 2127.515 kb on - strand at 2127.528 kb on - strand at 2127.608 kb on + strand at 2127.608 kb on + strand at 2127.608 kb on + strand at 2127.609 kb on - strand at 2127.612 kb on + strand at 2127.627 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,125,107 + EX28DRAFT_2101 0.18 +0.9 2,125,176 - EX28DRAFT_2101 0.25 +0.6 2,125,211 + EX28DRAFT_2101 0.29 +0.3 2,125,312 + EX28DRAFT_2101 0.40 -0.5 2,125,312 + EX28DRAFT_2101 0.40 -1.3 2,125,313 - EX28DRAFT_2101 0.40 -1.4 2,125,313 - EX28DRAFT_2101 0.40 -0.3 2,125,568 + EX28DRAFT_2101 0.67 +0.6 2,125,633 + EX28DRAFT_2101 0.74 +0.4 2,125,637 - EX28DRAFT_2101 0.75 -1.7 2,125,734 - EX28DRAFT_2101 0.85 -1.3 2,125,735 + EX28DRAFT_2101 0.85 +0.8 2,125,735 + EX28DRAFT_2101 0.85 -0.5 2,125,735 + EX28DRAFT_2101 0.85 -0.6 2,125,735 + EX28DRAFT_2101 0.85 -0.1 2,125,735 + EX28DRAFT_2101 0.85 -0.5 2,125,736 - EX28DRAFT_2101 0.86 +0.5 2,125,736 - EX28DRAFT_2101 0.86 +0.3 2,125,736 - EX28DRAFT_2101 0.86 -0.8 2,125,834 - -0.7 2,125,888 + -2.3 2,125,889 - -1.1 2,125,891 - -2.6 2,125,891 - +0.5 2,125,948 + -0.1 2,125,948 + -0.9 2,125,959 - -2.1 2,126,045 - EX28DRAFT_2102 0.18 -0.3 2,126,241 - EX28DRAFT_2102 0.45 +0.5 2,126,257 + EX28DRAFT_2102 0.47 -1.0 2,126,257 + EX28DRAFT_2102 0.47 +0.3 2,126,258 - EX28DRAFT_2102 0.47 -0.1 2,126,546 + EX28DRAFT_2102 0.87 -0.1 2,126,625 + +1.2 2,126,682 + +0.4 2,126,683 - +0.5 2,126,704 - -0.7 2,126,704 - +0.6 2,126,704 - -2.3 2,126,718 - +0.2 2,126,718 - +0.3 2,126,723 + +0.4 2,126,723 + +0.1 2,126,723 + -0.3 2,126,724 - -0.4 2,126,728 + -0.1 2,126,779 + +0.2 2,126,779 + -1.1 2,126,780 - +0.4 2,126,780 - +0.2 2,126,780 - +1.5 2,126,781 + +1.1 2,126,781 + +0.5 2,126,782 - +0.8 2,126,782 - -0.3 2,126,782 - +0.1 2,126,782 - -0.1 2,126,788 + -0.1 2,126,789 - +1.1 2,127,091 + +1.7 2,127,125 + +0.6 2,127,147 + -0.3 2,127,156 + +1.9 2,127,157 - +0.3 2,127,170 + EX28DRAFT_2104 0.12 -0.1 2,127,170 + EX28DRAFT_2104 0.12 +0.3 2,127,171 - EX28DRAFT_2104 0.13 -0.7 2,127,247 + EX28DRAFT_2104 0.33 +0.2 2,127,251 + EX28DRAFT_2104 0.34 -0.7 2,127,251 + EX28DRAFT_2104 0.34 +0.4 2,127,251 + EX28DRAFT_2104 0.34 -0.2 2,127,252 - EX28DRAFT_2104 0.34 +0.4 2,127,447 + EX28DRAFT_2104 0.86 +0.0 2,127,489 + -0.1 2,127,489 + -2.3 2,127,514 + -0.3 2,127,514 + +0.3 2,127,515 - +1.0 2,127,528 - +1.3 2,127,608 + -0.3 2,127,608 + -0.6 2,127,608 + -0.5 2,127,609 - +0.0 2,127,612 + -1.5 2,127,627 - -2.0
Or see this region's nucleotide sequence