Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2025 and EX28DRAFT_2026 are separated by 111 nucleotides EX28DRAFT_2026 and EX28DRAFT_2027 are separated by 185 nucleotides EX28DRAFT_2027 and EX28DRAFT_2028 are separated by 69 nucleotides
EX28DRAFT_2025: EX28DRAFT_2025 - ribosomal protein S1, at 2,036,456 to 2,038,129
_2025
EX28DRAFT_2026: EX28DRAFT_2026 - cytidylate kinase, at 2,038,241 to 2,038,924
_2026
EX28DRAFT_2027: EX28DRAFT_2027 - 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19), at 2,039,110 to 2,040,393
_2027
EX28DRAFT_2028: EX28DRAFT_2028 - phosphoserine aminotransferase apoenzyme (EC 2.6.1.52), at 2,040,463 to 2,041,515
_2028
Position (kb)
2039
2040
2041 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2038.937 kb on - strand at 2038.937 kb on - strand at 2038.941 kb on - strand at 2038.941 kb on - strand at 2038.974 kb on + strand at 2038.975 kb on - strand at 2038.975 kb on - strand at 2038.997 kb on + strand at 2039.015 kb on - strand at 2039.038 kb on - strand at 2039.103 kb on + strand at 2039.103 kb on + strand at 2039.104 kb on - strand at 2039.122 kb on - strand at 2039.122 kb on - strand at 2039.124 kb on + strand at 2039.125 kb on - strand at 2039.236 kb on - strand at 2039.301 kb on - strand, within EX28DRAFT_2027 at 2039.390 kb on + strand, within EX28DRAFT_2027 at 2039.390 kb on + strand, within EX28DRAFT_2027 at 2039.390 kb on + strand, within EX28DRAFT_2027 at 2039.614 kb on + strand, within EX28DRAFT_2027 at 2039.795 kb on + strand, within EX28DRAFT_2027 at 2039.835 kb on + strand, within EX28DRAFT_2027 at 2039.922 kb on + strand, within EX28DRAFT_2027 at 2040.376 kb on + strand at 2040.418 kb on + strand at 2040.419 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,038,937 - +0.6 2,038,937 - +0.3 2,038,941 - +1.0 2,038,941 - -0.1 2,038,974 + +1.3 2,038,975 - -0.2 2,038,975 - -0.1 2,038,997 + -1.1 2,039,015 - -1.1 2,039,038 - +0.8 2,039,103 + +0.5 2,039,103 + -0.0 2,039,104 - +0.4 2,039,122 - -1.1 2,039,122 - +0.4 2,039,124 + -5.2 2,039,125 - -0.9 2,039,236 - -4.3 2,039,301 - EX28DRAFT_2027 0.15 -3.1 2,039,390 + EX28DRAFT_2027 0.22 -3.5 2,039,390 + EX28DRAFT_2027 0.22 -2.9 2,039,390 + EX28DRAFT_2027 0.22 -3.3 2,039,614 + EX28DRAFT_2027 0.39 -5.0 2,039,795 + EX28DRAFT_2027 0.53 -3.3 2,039,835 + EX28DRAFT_2027 0.56 -4.6 2,039,922 + EX28DRAFT_2027 0.63 -5.8 2,040,376 + -5.2 2,040,418 + -4.3 2,040,419 - -1.4
Or see this region's nucleotide sequence