Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2015 and EX28DRAFT_2016 are separated by 124 nucleotides EX28DRAFT_2016 and EX28DRAFT_2017 overlap by 24 nucleotides EX28DRAFT_2017 and EX28DRAFT_2018 are separated by 141 nucleotides EX28DRAFT_2018 and EX28DRAFT_2019 overlap by 4 nucleotides
EX28DRAFT_2015: EX28DRAFT_2015 - 2-polyprenyl-3-methyl-5-hydroxy-6-metoxy-1,4-benzoquinol methylase, at 2,025,817 to 2,026,596
_2015
EX28DRAFT_2016: EX28DRAFT_2016 - Uncharacterized conserved protein, at 2,026,721 to 2,027,500
_2016
EX28DRAFT_2017: EX28DRAFT_2017 - Phosphotransferase enzyme family, at 2,027,477 to 2,028,370
_2017
EX28DRAFT_2018: EX28DRAFT_2018 - 3-deoxy-D-manno-octulosonate cytidylyltransferase, at 2,028,512 to 2,029,258
_2018
EX28DRAFT_2019: EX28DRAFT_2019 - Uncharacterized conserved protein, at 2,029,255 to 2,029,437
_2019
Position (kb)
2027
2028
2029 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2026.670 kb on + strand at 2026.670 kb on + strand at 2026.670 kb on + strand at 2026.671 kb on - strand at 2026.674 kb on - strand at 2026.674 kb on - strand at 2026.683 kb on - strand at 2026.683 kb on - strand at 2026.693 kb on + strand at 2026.693 kb on + strand at 2026.693 kb on + strand at 2026.693 kb on + strand at 2026.694 kb on - strand at 2026.694 kb on - strand at 2026.695 kb on + strand at 2026.695 kb on + strand at 2026.696 kb on - strand at 2026.696 kb on - strand at 2026.736 kb on - strand at 2026.742 kb on + strand at 2026.743 kb on - strand at 2026.743 kb on - strand at 2026.846 kb on + strand, within EX28DRAFT_2016 at 2026.849 kb on + strand, within EX28DRAFT_2016 at 2026.867 kb on + strand, within EX28DRAFT_2016 at 2026.909 kb on - strand, within EX28DRAFT_2016 at 2026.961 kb on + strand, within EX28DRAFT_2016 at 2026.962 kb on - strand, within EX28DRAFT_2016 at 2026.985 kb on + strand, within EX28DRAFT_2016 at 2026.985 kb on + strand, within EX28DRAFT_2016 at 2026.987 kb on + strand, within EX28DRAFT_2016 at 2026.987 kb on + strand, within EX28DRAFT_2016 at 2026.987 kb on + strand, within EX28DRAFT_2016 at 2026.987 kb on + strand, within EX28DRAFT_2016 at 2026.988 kb on - strand, within EX28DRAFT_2016 at 2027.212 kb on + strand, within EX28DRAFT_2016 at 2027.419 kb on + strand, within EX28DRAFT_2016 at 2027.578 kb on - strand, within EX28DRAFT_2017 at 2027.628 kb on + strand, within EX28DRAFT_2017 at 2027.639 kb on + strand, within EX28DRAFT_2017 at 2027.640 kb on - strand, within EX28DRAFT_2017 at 2027.777 kb on - strand, within EX28DRAFT_2017 at 2027.851 kb on + strand, within EX28DRAFT_2017 at 2027.851 kb on + strand, within EX28DRAFT_2017 at 2027.851 kb on + strand, within EX28DRAFT_2017 at 2027.852 kb on - strand, within EX28DRAFT_2017 at 2028.270 kb on + strand, within EX28DRAFT_2017 at 2028.271 kb on - strand, within EX28DRAFT_2017 at 2028.271 kb on - strand, within EX28DRAFT_2017 at 2028.324 kb on - strand at 2028.371 kb on - strand at 2028.379 kb on - strand at 2028.402 kb on + strand at 2028.403 kb on - strand at 2028.403 kb on - strand at 2028.405 kb on - strand at 2028.468 kb on - strand at 2028.514 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 2,026,670 + +0.3 2,026,670 + -1.2 2,026,670 + -0.2 2,026,671 - +0.2 2,026,674 - -0.5 2,026,674 - -0.6 2,026,683 - -1.8 2,026,683 - +0.4 2,026,693 + +2.1 2,026,693 + +0.1 2,026,693 + -0.6 2,026,693 + -0.0 2,026,694 - +0.7 2,026,694 - +0.1 2,026,695 + +0.1 2,026,695 + -0.8 2,026,696 - +0.0 2,026,696 - +0.3 2,026,736 - +0.4 2,026,742 + +0.4 2,026,743 - +0.3 2,026,743 - +0.6 2,026,846 + EX28DRAFT_2016 0.16 -0.4 2,026,849 + EX28DRAFT_2016 0.16 -0.0 2,026,867 + EX28DRAFT_2016 0.19 +0.5 2,026,909 - EX28DRAFT_2016 0.24 +0.4 2,026,961 + EX28DRAFT_2016 0.31 -0.1 2,026,962 - EX28DRAFT_2016 0.31 +1.1 2,026,985 + EX28DRAFT_2016 0.34 -0.2 2,026,985 + EX28DRAFT_2016 0.34 +0.3 2,026,987 + EX28DRAFT_2016 0.34 +0.1 2,026,987 + EX28DRAFT_2016 0.34 +0.2 2,026,987 + EX28DRAFT_2016 0.34 -0.8 2,026,987 + EX28DRAFT_2016 0.34 +0.2 2,026,988 - EX28DRAFT_2016 0.34 -1.2 2,027,212 + EX28DRAFT_2016 0.63 +0.1 2,027,419 + EX28DRAFT_2016 0.89 +0.1 2,027,578 - EX28DRAFT_2017 0.11 -0.7 2,027,628 + EX28DRAFT_2017 0.17 -3.1 2,027,639 + EX28DRAFT_2017 0.18 +0.1 2,027,640 - EX28DRAFT_2017 0.18 -0.5 2,027,777 - EX28DRAFT_2017 0.34 -0.5 2,027,851 + EX28DRAFT_2017 0.42 -3.3 2,027,851 + EX28DRAFT_2017 0.42 -0.3 2,027,851 + EX28DRAFT_2017 0.42 -0.1 2,027,852 - EX28DRAFT_2017 0.42 -1.2 2,028,270 + EX28DRAFT_2017 0.89 -2.0 2,028,271 - EX28DRAFT_2017 0.89 -0.6 2,028,271 - EX28DRAFT_2017 0.89 +0.6 2,028,324 - +0.2 2,028,371 - -0.5 2,028,379 - +0.2 2,028,402 + +0.1 2,028,403 - +0.3 2,028,403 - -2.3 2,028,405 - -0.1 2,028,468 - +0.4 2,028,514 - -3.9
Or see this region's nucleotide sequence