Experiment: M9 with sucrose and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1964 and EX28DRAFT_1965 are separated by 54 nucleotides EX28DRAFT_1965 and EX28DRAFT_1966 overlap by 4 nucleotides EX28DRAFT_1966 and EX28DRAFT_1967 are separated by 308 nucleotides
EX28DRAFT_1964: EX28DRAFT_1964 - Integral membrane protein, interacts with FtsH, at 1,967,392 to 1,968,051
_1964
EX28DRAFT_1965: EX28DRAFT_1965 - hypothetical protein, at 1,968,106 to 1,968,582
_1965
EX28DRAFT_1966: EX28DRAFT_1966 - DNA-binding winged-HTH domains, at 1,968,579 to 1,969,334
_1966
EX28DRAFT_1967: EX28DRAFT_1967 - hypothetical protein, at 1,969,643 to 1,970,386
_1967
Position (kb)
1968
1969
1970 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1967.617 kb on - strand, within EX28DRAFT_1964 at 1967.653 kb on + strand, within EX28DRAFT_1964 at 1967.680 kb on + strand, within EX28DRAFT_1964 at 1967.680 kb on + strand, within EX28DRAFT_1964 at 1967.738 kb on + strand, within EX28DRAFT_1964 at 1967.759 kb on - strand, within EX28DRAFT_1964 at 1967.963 kb on - strand, within EX28DRAFT_1964 at 1967.963 kb on - strand, within EX28DRAFT_1964 at 1967.964 kb on + strand, within EX28DRAFT_1964 at 1967.986 kb on + strand at 1967.986 kb on + strand at 1967.987 kb on - strand at 1967.998 kb on + strand at 1967.998 kb on + strand at 1967.998 kb on + strand at 1967.999 kb on - strand at 1968.062 kb on + strand at 1968.107 kb on + strand at 1968.123 kb on + strand at 1968.123 kb on + strand at 1968.125 kb on + strand at 1968.126 kb on - strand at 1968.126 kb on - strand at 1968.126 kb on - strand at 1968.188 kb on + strand, within EX28DRAFT_1965 at 1968.188 kb on + strand, within EX28DRAFT_1965 at 1968.189 kb on - strand, within EX28DRAFT_1965 at 1968.189 kb on - strand, within EX28DRAFT_1965 at 1968.192 kb on - strand, within EX28DRAFT_1965 at 1968.192 kb on - strand, within EX28DRAFT_1965 at 1968.237 kb on + strand, within EX28DRAFT_1965 at 1968.238 kb on - strand, within EX28DRAFT_1965 at 1968.270 kb on - strand, within EX28DRAFT_1965 at 1968.313 kb on - strand, within EX28DRAFT_1965 at 1968.371 kb on + strand, within EX28DRAFT_1965 at 1968.371 kb on + strand, within EX28DRAFT_1965 at 1968.371 kb on + strand, within EX28DRAFT_1965 at 1968.371 kb on + strand, within EX28DRAFT_1965 at 1968.371 kb on + strand, within EX28DRAFT_1965 at 1968.372 kb on - strand, within EX28DRAFT_1965 at 1968.405 kb on - strand, within EX28DRAFT_1965 at 1968.515 kb on + strand, within EX28DRAFT_1965 at 1968.519 kb on - strand, within EX28DRAFT_1965 at 1968.574 kb on + strand at 1968.600 kb on - strand at 1968.601 kb on + strand at 1968.602 kb on - strand at 1968.606 kb on + strand at 1968.606 kb on + strand at 1968.606 kb on + strand at 1968.607 kb on - strand at 1968.607 kb on - strand at 1968.607 kb on - strand at 1968.697 kb on + strand, within EX28DRAFT_1966 at 1968.698 kb on - strand, within EX28DRAFT_1966 at 1968.874 kb on + strand, within EX28DRAFT_1966 at 1968.874 kb on + strand, within EX28DRAFT_1966 at 1968.875 kb on - strand, within EX28DRAFT_1966 at 1969.127 kb on + strand, within EX28DRAFT_1966 at 1969.174 kb on - strand, within EX28DRAFT_1966 at 1969.209 kb on - strand, within EX28DRAFT_1966 at 1969.251 kb on + strand, within EX28DRAFT_1966 at 1969.254 kb on + strand, within EX28DRAFT_1966 at 1969.255 kb on - strand, within EX28DRAFT_1966 at 1969.281 kb on + strand at 1969.281 kb on + strand at 1969.282 kb on - strand at 1969.282 kb on - strand at 1969.658 kb on - strand at 1969.683 kb on - strand at 1969.846 kb on + strand, within EX28DRAFT_1967 at 1970.038 kb on + strand, within EX28DRAFT_1967 at 1970.170 kb on - strand, within EX28DRAFT_1967 at 1970.286 kb on + strand, within EX28DRAFT_1967 at 1970.321 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with sucrose and trichloroethylene 76.3 uM remove 1,967,617 - EX28DRAFT_1964 0.34 -0.1 1,967,653 + EX28DRAFT_1964 0.40 +0.6 1,967,680 + EX28DRAFT_1964 0.44 -1.2 1,967,680 + EX28DRAFT_1964 0.44 -0.4 1,967,738 + EX28DRAFT_1964 0.52 -0.6 1,967,759 - EX28DRAFT_1964 0.56 +0.5 1,967,963 - EX28DRAFT_1964 0.87 -3.8 1,967,963 - EX28DRAFT_1964 0.87 -0.5 1,967,964 + EX28DRAFT_1964 0.87 -0.8 1,967,986 + +0.0 1,967,986 + +0.5 1,967,987 - -1.6 1,967,998 + -1.1 1,967,998 + -0.7 1,967,998 + +0.2 1,967,999 - +1.0 1,968,062 + -0.4 1,968,107 + +1.2 1,968,123 + -0.5 1,968,123 + -0.2 1,968,125 + +0.8 1,968,126 - -1.8 1,968,126 - +0.5 1,968,126 - -0.3 1,968,188 + EX28DRAFT_1965 0.17 +0.8 1,968,188 + EX28DRAFT_1965 0.17 -1.2 1,968,189 - EX28DRAFT_1965 0.17 -1.6 1,968,189 - EX28DRAFT_1965 0.17 +0.4 1,968,192 - EX28DRAFT_1965 0.18 -0.2 1,968,192 - EX28DRAFT_1965 0.18 +0.3 1,968,237 + EX28DRAFT_1965 0.27 -0.6 1,968,238 - EX28DRAFT_1965 0.28 +0.1 1,968,270 - EX28DRAFT_1965 0.34 +2.5 1,968,313 - EX28DRAFT_1965 0.43 -2.7 1,968,371 + EX28DRAFT_1965 0.56 -1.2 1,968,371 + EX28DRAFT_1965 0.56 +0.4 1,968,371 + EX28DRAFT_1965 0.56 +0.5 1,968,371 + EX28DRAFT_1965 0.56 -0.2 1,968,371 + EX28DRAFT_1965 0.56 -0.7 1,968,372 - EX28DRAFT_1965 0.56 -0.1 1,968,405 - EX28DRAFT_1965 0.63 +0.4 1,968,515 + EX28DRAFT_1965 0.86 -0.0 1,968,519 - EX28DRAFT_1965 0.87 +0.4 1,968,574 + -1.9 1,968,600 - +1.1 1,968,601 + +0.1 1,968,602 - +0.1 1,968,606 + -3.8 1,968,606 + +0.3 1,968,606 + -1.9 1,968,607 - -0.2 1,968,607 - -1.9 1,968,607 - +0.9 1,968,697 + EX28DRAFT_1966 0.16 -0.5 1,968,698 - EX28DRAFT_1966 0.16 -0.9 1,968,874 + EX28DRAFT_1966 0.39 +0.5 1,968,874 + EX28DRAFT_1966 0.39 -0.3 1,968,875 - EX28DRAFT_1966 0.39 -0.3 1,969,127 + EX28DRAFT_1966 0.72 -3.9 1,969,174 - EX28DRAFT_1966 0.79 +0.0 1,969,209 - EX28DRAFT_1966 0.83 -1.1 1,969,251 + EX28DRAFT_1966 0.89 -0.5 1,969,254 + EX28DRAFT_1966 0.89 -0.8 1,969,255 - EX28DRAFT_1966 0.89 +1.1 1,969,281 + -0.1 1,969,281 + +0.3 1,969,282 - +0.5 1,969,282 - -0.3 1,969,658 - -0.2 1,969,683 - -1.8 1,969,846 + EX28DRAFT_1967 0.27 -1.3 1,970,038 + EX28DRAFT_1967 0.53 -0.1 1,970,170 - EX28DRAFT_1967 0.71 -0.5 1,970,286 + EX28DRAFT_1967 0.86 -1.4 1,970,321 - -1.3
Or see this region's nucleotide sequence